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DOI :
10.3791/3932-v
•
11:28 min
March 31st, 2012
Chapters
0:05
Title
1:46
Transformation of E. coli with the pGEX-RhoA(G17A) Construct
2:55
Preparation of GST-RhoA(G17A) Beads
7:50
GEF Pulldown Assay with Nucleotide-free RhoA(G17A) Beads
10:11
Representative Pull Down Results
11:03
Conclusion
这里介绍的方法检测,按照激活RhoA的具体GDP / GTP交换因子(全环基金)在培养细胞中的突变RhoA的GST融合蛋白具有高亲和力激活全环基金的使用。全环基金沉淀的细胞裂解物,免疫印迹和密度定量检测。
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