Name: determining soil-transmitted helminth infection status and physical fitness of school-aged children
Uploaded: 2012-08-22T12:00:00
Description: Watch this Scientific Journal Video about Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children at JoVE.com

作者感谢云南，中华人民共和国和阿博维尔，科特迪瓦，他们参与上述研究的社区。我们非常感激的测试和执行的协议，协助当地的野队。我们特别感谢下列同事从瑞士热带和公共健康研究所：Endriss伊薇特提供在视频拍摄过程中的显微图像，Hanspeter实验室的支持和孙燕姿克劳思·马蒂和他的团队对她的帮助。最后，我们感谢两位匿名审稿进行了一系列有益的意见。

1。改良加藤技术<ol><li>将一个标准的改良加藤模板为41.7毫克（mg），大便显微镜载片上有一个洞。 </li><li>炒到2-3克（g）的新鲜粪便样品放在一张报纸或铝箔，然后按顶部的金属丝或塑料网筛它一块。 </li><li>使用一个小的塑料小铲，刮离网筛分的材料，并完全填充改良加藤模板的孔中。以除去过量的粪便物，平与刮刀的孔的含量。 </li><li>垂直删除，而不会干扰现在坚持粪便的显微镜幻灯片模板...

<ol>
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本文中所描述的三个协议已经过测试，并执行在西南部云南省的一部分，中华人民共和国各成员之间的布朗族少数民族21在不同地区的非洲6,13,14。 有几个模板，可用于改良加藤技术。每一个不同的孔尺寸将提供一个独特的粪便量。对于计算的EPG，因此，有必要知道单元的模板，以乘以鸡蛋的计数量的修正因子，以到达的EPG估计。此外，当在社区钩虫是本调查?...

注意：在此列表中的材料是通用的，可以从不同的来源获得。 <table border="1"><tbody><tr><td> 的试剂的名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td>改良加藤试剂盒 [400带有一个孔的6毫米（mm）（直径）在1.5毫米厚的模板塑料模板400塑料抹刀; 80网目尺寸的尼龙筛20米的作用; 20米的作用的亲水性的玻璃纸，34微米厚。] </td><td>韦斯特弗兰德森</td><td></td><td>请注意的甘油孔雀石绿溶液不包含在该试剂盒中，但可以从任何化学品供应商购买。 </td></tr><tr><td>玻璃漏斗[约8厘米（cm），宽6厘米深] </td><td></td><td></td><td>为贝尔曼技术在玻璃漏斗应该是能够容纳约60毫升水。钍E尺寸的橡胶软管和纱布是依赖于所使用的玻璃漏斗的类型。 </td></tr><tr><td>生物显微镜</td><td>奥林巴斯</td><td> CX21LED/CX21 </td><td></td></tr><tr><td>离心分离</td><td>上海医疗器械集团</td><td> 80 - 2T </td><td></td></tr><tr><td>预先录制的信号</td><td> Bitworks设计</td><td>团队哔哔测试版1.3.1 </td><td> iPhone应用程序</td></tr><tr><td>心脏监测仪</td><td> POLAR </td><td> FT1 </td><td>手表和发射器</td></tr></tbody></table>

determining soil-transmitted helminth infection status and physical fitness of school-aged children

土壤传播的蠕虫（STH）感染是常见的。事实上，超过1亿人受到影响，主要是在发展中世界，那里的贫困盛行，卫生行为，供水和卫生设施往往缺乏1,2， 蛔虫 ， 鞭虫和钩虫种类， 十二指肠钩虫 ， 钩虫美洲 ，是最普遍的STHs 3。由于钩虫病，蛔虫病和鞭虫病的全球疾病负担的估计是22.1，10.5，和640万伤残调整寿命年（DALY），分别为4。此外，估计有30-100亿人感染粪类圆线虫 ，最被忽视的STH种具有全球意义，可以说是也造成相当大的公众健康的影响5,6。多物种感染（即不同的STHs窝藏在一个单一的个体）是常见的，已经感染林<html>糟透了降低生产率，从而促进经济的发展中国家的前景1,3。 的诊断的常见STHs的，世界卫生组织（WHO）建议的改良加藤技术7.8，这是一个相对简单的方法来确定这类感染的发生率和强度。它促进了检测，感染者通过在其粪便中的寄生虫卵。 随着的诊断S.stercoralis方面，目前还没有简单而准确的工具。的的贝尔曼技术是应用最广泛的方法，其诊断。的贝尔曼技术背后的原理是，积极S.stercoralis幼虫迁移出照明的新鲜粪便样品的幼虫趋光9。它需要不太复杂的实验材料和消耗更少的时间比文化和免疫学方法5。 T“发病率与运输及房屋局局长感染范围从急性，但共同的症状，如腹痛，腹泻，皮肤瘙痒，慢性症状，如贫血，不足和营养不良，和认知功能障碍10。一般都是非特异性的症状，微妙的，他们经常被忽视，被认为是一个正常的条件下受影响的个体，或者被处理作为可能会对多见于一个给定的设定的其他疾病的症状，因此，可以想象的是，真正的负担被低估了评估的STH感染工具依靠自我宣称的症状和体征通常情况下人口为基础的调查。 在20世纪80年代末和90年代初，Stephenson和他的同事们强调STH感染的可能性降低了体能的男孩6-12岁11,12。最近13,14,15这条线的科学调查获得了新的动力。 20米（m）折返跑测试开发ð验证莱热等。，是全球通用的衡量有氧健身的儿童17。测试是简单的规范和可以执行的地方有20米长，平坦的运行过程和音频源，使得它的使用有吸引力的资源限制设置13。为方便和规范的尝试，在评估是否STH感染有效果的适龄儿童的体能，我们提出的方法，诊断STH感染或措施的体能是简单的执行，提供精确和可重复的结果。这将有助于产生新的证据，STH感染对健康的影响。

Watch this Scientific Journal Video about Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children at JoVE.com

Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children

用土壤传播的的蠕虫（STHs）的慢性感染引起吸收不良，发育迟缓，浪费在成长中的孩子。因此，它是合理的，这些感染也可以减少儿童的体能。在这里，我们可视化技术的诊断STHs和20米折返跑测试，以评估儿童的身体健康。

确定土壤传播的蠕虫感染状况和体质的学龄儿童

Soil-transmitted helminth (STH) infections are common. Indeed, more than 1 billion people are affected, mainly in the developing world where poverty ...

Research

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确定使用血凝检测血清中的反应和滴度

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Determining the Phagocytic Activity of Clinical Antibody Samples

确定临床抗体样品的吞噬活性

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Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

从使用鸡蛋<em&gt;血吸虫</em&gt;作为<em&gt;在体内</em&gt;蠕虫引起的肺部炎症模型

Schistosoma parasites are blood flukes that infect an estimated 200 million people worldwide 1. In chronic infection with Schistosoma, the severe ...

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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

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In this manuscript, we describe a transposon sequencing (Tn-Seq) technique to identify and quantify Leptospira interrogans mutants altered in fitness ...