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JoVE Journal

Neuroscience

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In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development

11.3K Views

08:57 min

August 3rd, 2012

August 3rd, 2012

11,323 Views

0:05

Title

1:34

Egg Preparation

2:42

Glass Injection Needle Preparation

3:25

Midbrain Injection

4:38

Electroporation

5:39

Incubate and Harvest

6:47

Results: Embryonic Chick Midbrain In ovo Electroporation Assay

8:13

Conclusion

To assess the function and the regulation of genes during the development of midbrain dopaminergic neurons, we describe a method that involves in ovo electroporation of plasmid DNA constructs into embryonic chick ventral midbrain dopaminergic neuron progenitors. This technique can be used to achieve efficient expression of genes of interest to study different aspects of midbrain development and dopaminergic neuron differentiation.

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