Name: functional neuroimaging using ultrasonic blood-brain barrier disruption and manganese-enhanced mri
Uploaded: 2012-07-12T13:00:00
Description: Watch this Scientific Journal Video about Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI at JoVE.com

所有工作是在公爵在体内显微镜中心，美国国立卫生研究院/ NIBIB 国家生物医学技术资源中心（P41 EB015897）和国家癌症研究所的小动物成像资源计划（U24 CA092656）。 NSF研究生研究奖学金（2003014921），提供额外的支持。

1。组装和校准超声诊断系统<ol><li>直径宽，足以覆盖的小鼠大脑和中心频率在2 MHz的范围内与单元素的超声换能器的超声系统开始。传感器是由50分贝功率的放大器，它是连接到一个信号发生器产生超声波脉冲序列。 </li><li>要校准的超声波系统的声压，使用与施加电压产生的声压水听器。传感器放置在一个以上的水听器的水箱。申请一个简单的脉冲（例如，换能器的频率在10周期与脉冲重复频率...

<ol>
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在这里，一个方法是为无创性开放血脑屏障贯穿整个鼠脑超声和微泡（BOMUS）。随着BBB开放，锰2 +管理和激活诱导锰增强磁共振成像（MRI检查目的）被用来形象短时间刺激在轻轻镇静小鼠的神经元反应。 取得足够的血脑屏障开放与负峰值声压为0.36兆帕。请注意，这是在超声束的中心在头皮表面的压力。单元素的传感器的光束轮廓的测量表明，在光束边缘的声压​​是...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td>水听器</td><td>索诺拉州医疗系统，朗蒙特，CA </td><td>四至-251的SN </td><td></td></tr><tr><td>翻译阶段</td><td> Newport公司，加利福尼亚州欧文</td><td></td><td></td></tr><tr><td>超声换能器</td><td>奥林巴斯公司，无损检测，沃尔瑟姆马</td><td> A306S-SU </td><td>回顾伴随传感器找到确切的中心频率，特别是传感器，对目录中所列的标称频率可能不同于制造商的测试表。 （例如，我们的传感器的标称频率为2.25 MHz的，但实际的中心频率为2.15 MHz的。） </td></tr><tr><td> VEVO影像站</td><td> VisualSonics，Inc。加拿大多伦多</td><td>; </td><td></td></tr><tr><td> 50 dB的功率放大器</td><td> E和我，纽约州罗切斯特市</td><td>模型240L </td><td></td></tr><tr><td>信号发生器</td><td>安捷伦科技公司，加利福尼亚州圣克拉拉</td><td>型号33220A </td><td></td></tr><tr><td> MnCl 2 - （H2O）4 </td><td>西格玛</td><td></td><td>分子量不同批次，要求制造商精确测量</td></tr><tr><td> perflutren脂微</td><td>马北比尔里卡，医学影像，lantheus </td><td> DEFINITY的</td><td></td></tr><tr><td>微搅拌器</td><td>马北比尔里卡，医学影像，lantheus </td><td> VIALMIX </td><td></td></tr><tr><td>磁共振成像线圈</td><td> M2M影像公司，Hillcrest的，OH </td><td></td><td> 35毫米直径的正交传输/接收量线圈</td></tr><tr><td>磁共振成像系统</td><td> GE医疗，MilwaukeE，WI </td><td></td><td> GE EXCITE控制台的操作7-T的水平孔磁铁</td></tr><tr><td>图像分析环境</td><td>面相影像，加利福尼亚州圣迭戈，MathWorks公司，Natic​​k市马</td><td>阿米拉的MATLAB </td><td></td></tr></tbody></table>

functional neuroimaging using ultrasonic blood-brain barrier disruption and manganese-enhanced mri

虽然老鼠是主导的模型系统，为研究神经科学，脑功能成像在小鼠遗传和分子基础仍然是技术上的挑战。一种方法，激活诱导锰增强磁共振成像（MRI检查目的），已成功地用于映射在啮齿类动物的神经元活动1-5。在AIM MRI，锰2 +作用钙模拟，并在6,7去极化神经元累积。由于锰2 +缩短的T 1组织的财产，升高的神经元活动的地区，将加强在MRI。此外，锰2 +清除慢慢从活化地区，因此，可以进行刺激之前成像磁铁外，从而提高实验的灵活性。然而，因为锰2 +不容易穿过血脑屏障（BBB）的，需要打开血脑屏障限制使用的AIM MRI，尤其是在小鼠。 开放血脑屏障的工具之一是ULTrasound。虽然潜在的破坏性，如果超声结合管理与充气微泡（即超声造影剂），血脑屏障开放所需的声压是相当低的。可以使用这种超声和微泡的结合，可靠地打开，而不会造成组织损伤8-11血脑屏障。 在这里，一种方法是执行旨在利用微气泡和超声波打开血脑屏障的MRI。静脉注射perflutren微泡后，重点不突出的脉冲超声波束被剃光鼠标头3分钟。为简单起见，我们称这BBB的开放的技术与微泡和超声BOMUS 12。使用BOMUS打开整个血脑屏障的两个大脑半球，锰是管理整个鼠脑。轻轻镇静小鼠的实验刺激后，AIM MRI是用来映射神经反应。 至证明这种方法，本BOMUS和AIM MRI用于映射单方面的触须轻轻镇静小鼠13机械刺激。因为BOMUS可以打开整个两半球血脑屏障，无刺激大脑的一侧是用来控制背景非特异性刺激。由此产生的3D激活地图同意的桶领域皮质14的触须地区出版的申述。在超声开放血脑屏障的是快速，非侵入性的，可逆的，因此这种方法是适用于高吞吐量和/或纵向研究在清醒小鼠。

Watch this Scientific Journal Video about Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI at JoVE.com

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

一个被描述为广泛的开放血脑屏障在鼠标使用微泡和超声技术。使用这种技术，锰，可管理的鼠脑。因为锰是一个磁共振造影剂，在去极化神经元的积累，这种方法能够使神经元的活动影像。

使用超声波血脑屏障破坏和锰增强MRI的脑功能成像

Although mice are the dominant model system for studying the genetic and molecular underpinnings of neuroscience, functional neuroimaging in mice remains ...

Research

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Neuroscience

Measuring the Subjective Value of Risky and Ambiguous Options using Experimental Economics and Functional MRI Methods

，测量主观价值风险和不明确的选项，使用实验经济学和功能磁共振成像的方法

Most of the choices we make have uncertain consequences. In some cases the probabilities for different possible outcomes are precisely known, a condition ...

科研

神经科学

Optogenetic Functional MRI

光遗传学功能磁共振成像

The investigation of the functional connectivity of precise neural circuits across the entire intact brain can be achieved through optogenetic functional ...

High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis

血脑屏障的高分辨率共聚焦成像: 成像、3D 重建和 Transcytosis 的量化

The blood-brain barrier (BBB) is a dynamic multicellular interface that regulates the transport of molecules between the circulation and the brain. ...

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

用荧光标记示踪剂对小鼠血脑屏障通透性的体内检测

Blood-brain barrier (BBB) is a specialized barrier that protects the brain microenvironment from toxins and pathogens in the circulation and maintains ...

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

一种体内评价大鼠缺血性中风模型中血脑屏障的破坏

Ischemic stroke leads to vasogenic cerebral edema and subsequent primary brain injury, which is mediated through destruction of the blood-brain barrier ...

Neuroimaging Field Methods Using Functional Near Infrared Spectroscopy (NIRS) Neuroimaging to Study Global Child Development: Rural Sub-Saharan Africa

Neuroimaging Field Methods Using Functional Near Infrared Spectroscopy NIRS Neuroimaging to Study Global Child Development: Rural Sub-Saharan Africa

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Portable neuroimaging approaches provide new advances to the study of brain function and brain development with previously inaccessible populations and in ...

A Benchtop Approach to the Location Specific Blood Brain Barrier Opening using Focused Ultrasound in a Rat Model

在鼠模型中使用聚焦超声波打开位置特定血脑屏障开口的台面方法

Stereotaxic surgery is the gold standard for localized drug and gene delivery to the rodent brain. This technique has many advantages over systemic ...

Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation

聚焦超声诱导血脑屏障打开，用于靶向大脑结构和评估化学遗传神经调控

Acoustically Targeted Chemogenetics (ATAC) allows for the noninvasive control of specific neural circuits. ATAC achieves such control through a ...

Role of Diffusion MRI Tractography in Endoscopic Endonasal Skull Base Surgery

扩散MRI造影术在内窥镜内窥镜颅骨基础外科中的作用

Endoscopic endonasal surgery has gained a prominent role in the management of complex skull base tumors. It allows the resection of a large group of ...

跨内皮抗性测量以评估细胞屏障完整性

Barrier Functional Integrity Recording on bEnd.3 Vascular Endothelial Cells via Transendothelial Electrical Resistance Detection

作者聚焦：TEER 检测在评估细胞屏障完整性中的应用 

通过跨内皮电阻检测对bEnd.3血管内皮细胞进行屏障功能完整性记录

The blood-brain barrier (BBB) is a dynamic physiological structure composed of microvascular endothelial cells, astrocytes, and pericytes. By coordinating ...