Immunhistokjemisk farging av B7-H1 (PD-L1) på parafin-embedded lysbilder av bukspyttkjertelen Adenocarcinoma Tissue
January 3rd, 2013
•Elaine Bigelow1,2, Katherine M. Bever1,2, Haiying Xu1,2,3, Allison Yager1, Annie Wu1,2,4, Janis Taube3,5, Lieping Chen6, Elizabeth M. Jaffee1,2,5,7,8, Robert A. Anders1,2,5,8, Lei Zheng1,2,4,5,7
1The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, 2Department of Oncology, The Johns Hopkins University School of Medicine, 3Department of Dermatology, The Johns Hopkins University School of Medicine, 4Department of Surgery, Johns Hopkins University School of Medicine, 5The Sol Goldman Pancreatic Cancer Center, The Johns Hopkins University School of Medicine, 6Yale Cancer Center, Yale School of Medicine, 7The Skip Viragh Center for Pancreatic Cancer, The Johns Hopkins University School of Medicine, 8Department of Pathology, The Johns Hopkins University School of Medicine
B7-H1 (PD-L1) og dens binding til PD-1 gi et stort tumorindusert immunsuppressivt signal i svulsten mikromiljøet. En immunhistokjemisk farging teknikk for å karakterisere uttrykk og lokalisering av B7-H1 i bukspyttkjertelen adenokarsinom er beskrevet her.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved