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15:01 min
August 6th, 2012
DOI :
10.3791/4168-v
Chapters
0:05
Title
2:53
Molecular Beacon Design
4:31
Nuclear Lysate Preparation
8:31
Molecular Beacon Assay
11:51
Representative Analysis of Tumor Cell Lysates
13:54
Conclusion
Summary
我们描述了DNA糖基化酶和AP内切酶的活动,在核细胞裂解定量,实时测量的方法。该法产生服从动力学分析DNA修复活动率,是适应组织和肿瘤裂解物或纯化蛋白质的DNA修复能力的量化。
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