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Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

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11:25 min

October 4th, 2012

October 4th, 2012

15,102 Views

0:05

Title

2:10

Protoplast Generation

4:23

FACS of Leaf Protoplasts

5:54

Results: Representative Analyses of GFP Expression in Flow Sorted Arabidopsis Protoplasts

10:26

Conclusion

A method for producing Arabidopsis leaf protoplasts that are compatible with fluorescence activated cell sorting (FACS), allowing for studies of specific cell populations. This method is compatible with any Arabidopsis line that expresses GFP in a subset of cells.

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