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DOI :
10.3791/4297-v
•
10:53 min
August 22nd, 2012
Chapters
0:05
Title
1:50
Culturing the Macrophage Cell Line RAW 264.7
3:52
Infection of RAW 264.7 Cells with MNV Inoculum
6:16
Preparation of Low Melting Point Agarose Overlay
7:34
Visualization of Plaques by Neutral Red Staining
8:49
Results: Images of Cell Monolayers Before and After Infection and Plaque Formation
9:58
Conclusion
在这里,我们描述了一种方法来量化小鼠诺罗病毒(MNV),这是唯一的,有效地复制在细胞培养中的诺罗病毒的感染性颗粒。噬斑分析MNV小鼠的巨噬细胞的趋利用,可适于使用的生物或环境样品,含有MNV。
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