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DOI :
10.3791/4297-v
•
10:53 min
August 22nd, 2012
Chapters
0:05
Title
1:50
Culturing the Macrophage Cell Line RAW 264.7
3:52
Infection of RAW 264.7 Cells with MNV Inoculum
6:16
Preparation of Low Melting Point Agarose Overlay
7:34
Visualization of Plaques by Neutral Red Staining
8:49
Results: Images of Cell Monolayers Before and After Infection and Plaque Formation
9:58
Conclusion
ここでは、効率的に細胞培養で複製のみノロウイルスですマウスノロウイルス(MNV)の感染性粒子を定量化する方法を説明します。プラークアッセイは、マウスのマクロファージのためにMNVの向性を活用し、MNVを含む生物学的または環境試料での使用に適合させることができる。
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