Name: design and assembly of an ultra-light motorized microdrive for chronic neural recordings in small animals
Uploaded: 2012-11-08T13:00:00
Description: Watch this Scientific Journal Video about Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals at JoVE.com

这项工作是由酯和约瑟夫·麦克奈特Klingenstein基金，捐赠基金，NINDS 1R01NS066408-01A1。

1。组件概述<ol><li>一个完整的微硬盘组成的几个主要部件（ 图1）：作为上层建筑具有精细螺纹输出轴的驱动器，电动机，带有电极和提供了一个电连接的点的螺纹班车的机箱，以及一个Omnetics（或同等学历）连接器。 </li><li>底盘，电极班车，电极穿梭管的自定义组件的设计（SolidWorks的三维CAD软件）和加工的精密加工。其它所有组件都是市售的。 </li><li>底盘和穿梭从轻量级的聚醚?...

     

<ol>
	<li>O'Keefe, J., Dostrovsky, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+hippocampus+as+a+spatial+map.">The hippocampus as a spatial map.</a> Brain Research. 34, 171-175 (1971).</li><li>Pennartz, C. M., Berke, J. D., Graybiel, A. M., Ito, R., Lansink, C. S., van der Meer, M., Redish, A. D., Smith, K. S., Voorn, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Corticostriatal Interactions during Learning, Memory Processing, and Decision. 29, 12831-12838 (2009).</li><li>Kepecs, A., Uchida, N., Zariwala, H., Mainen, Z. 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这里介绍的协议将导致一个能够以最小的运动伪影的高品质的录音设备，只有采取适当的照顾与建设。适合穿梭在机箱的如果至关重要太紧张，电机过载的风险是高过于宽松，显着的运动伪影的风险是高。一个理想的选择，可以让穿梭旅行的整个长度的螺纹轴的位置或抖动没有倾斜了。 记录电极的选择同样是重要的;材料，阻抗，绝缘，和小费档案中的选择可能会影响组织的?...

<table border="1"&gt;<tbody&gt;<tr&gt;<td<strong&gt;项目名称</strong</td&gt;<td<strong&gt;公司</strong</td&gt;<td<strong&gt;产品目录号</strong</td&gt;<td<strong&gt;评论</strong</td&gt;</tr&gt;<tr&gt;<td&gt;机壳</td&gt;<td&gt;自定义</td&gt;<td</td&gt;<td从PEI&gt;“剪切”</td&gt;</tr&gt;<tr&gt;<td&gt;电极班车</td&gt;<td&gt;自定义</td&gt;<td</td&gt;<td从PEI&gt;“剪切”</td&gt;</tr&gt;<tr&gt;<td&gt;接送管</td&gt;<td&gt;自定义</td&gt;<td</td&gt;<td剪切不锈钢</td&gt;</tr&gt;<tr&gt;<td&gt;连接​​器</td&gt;<td&gt; Omnetics</td&gt;<td&gt; A7886-001</td&gt;<td配合A7877-001</td&gt;</tr&gt;<tr&gt;<td&gt;电机/减速机</td&gt;<td&gt;冯哈伯</td&gt;<td&gt; 0206-A-001-B-021-47：1</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;电线指南</td&gt;<td&gt;小零件公司</td&gt;<td&gt; SWPT-0113-12</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;电极指南</td&gt;<td&gt;小零件公司</td&gt;<td&gt; SWPT-0045-12</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;10MΩ铂铱电极</td&gt;<td&gt;柱石，公司</td&gt;<td&gt; PI2PT310.0H3</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;铂金导丝</td&gt;<td&gt; AM系统</td&gt;<td&gt; 772000</td&gt;<td电极线</td&gt;</tr&gt;<tr&gt;<td&gt;银线</td&gt;<td&gt; AM系统</td&gt;<td&gt; 786000</td&gt;<td对于地线</td&gt;</tr&gt;<tr&gt;<td&gt;钨丝</td&gt;<td&gt; AM系统</td&gt;<td&gt; 797000</td&gt;<td&gt;电极针</td&gt;</tr&gt;<tr&gt;<td&gt;透明度</td&gt;<td&gt; 3M</td&gt;<td&gt; AF4300</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td乇封</td&gt;<td&gt;瓦里安</td&gt;<td&gt; 9530001</td&gt;<td</td&gt;</tr&gt;<tr&gt;<tdKWIK-主演</td&gt;<td&gt; WPI</td&gt;<tdKWIK-CAST</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;氰基丙烯酸酯</td&gt;<td&gt; KRAZY胶</td&gt;<td&gt; KG517</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;快速设置环氧</td&gt;<td&gt;哈德曼</td&gt;<td&gt; 04001</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;轻质矿物油</td&gt;<td&gt; SIGMA</td&gt;<td&gt; M5310</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;氯漂白剂</td&gt;<td</td&gt;<td</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;对角线切割机</td&gt;<td</td&gt;<td</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;手术刀</td&gt;<td</td&gt;<td</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;钳</td&gt;<td</td&gt;<td</td&gt;<td</td&gt;</tr&gt;<tr&gt;<td&gt;驱动器夹具</td&gt;<td&gt;自定义</td&gt;<td</td&gt;<td&gt;环氧交配连接器，注射器或棒</td&gt;</tr&gt;<tr&gt;<td&gt;小副</td&gt;<td</td&gt;<td</td&gt;<td</td&gt;</tr&gt;</tbody&gt;</table

design and assembly of an ultra-light motorized microdrive for chronic neural recordings in small animals

的能力，长期记录神经元在自由活动的动物的种群已被证明是非常宝贵的工具，用于解剖的神经回路的功能相关的各种自然行为，包括导航1，决策2,3，和上一代的复杂的运动序列4 ，5,6。在精密加工方面的进展已可用于制造重量轻的设备，适合慢性录音的小动物，如小鼠和鸣禽。调整电极的位置与远程控制电机的能力进一步提高各种行为背景的记录产量的减少动物的处理6,7。 在这里，我们描述了一个协议，以建立一个超轻型电动微硬盘为长期的慢性记录的小动物。我们的设计是从早前公布的第7版，已被改编为易用性和成本有效的veness更加实用和广泛的研究人员访问。这种成熟的设计8,9,10,11允许的精细电极，远程定位〜5毫米的范围内，重量不超过750毫克时完全组装。我们提出如何构建和组装这些驱动器，包括三维CAD图纸的所有自定义的微硬盘组件的完整的协议。

Watch this Scientific Journal Video about Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals at JoVE.com

Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals

一个超轻型电动微硬盘的设计，制造和装配。该设备提供了成本效益和易于使用的解决方案，单台小行为的动物慢性录音。

设计和组装的超轻型电动微型硬盘（Microdrive）慢性神经录音的小动物

The ability to chronically record from populations of neurons in freely behaving animals has proven an invaluable tool for dissecting the function of ...

Research

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Neuroscience

Electrode Fabrication and Implantation in Aplysia californica for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals

Electrode Fabrication and Implantation in Aplysia californica for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals

电极的制作及植入海兔californica完整的，自由的行为的动物多通道的神经和肌肉的录音

Recording from key nerves and muscles of Aplysia during feeding behavior allows us to study the patterns of neural control in an intact animal.  ...

科研

神经科学

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

一个慢性双光子薄颅骨窗口技术在体内 Neuroinflammation模型小鼠小胶质细胞成像

Traditionally in neuroscience, in vivo two photon imaging of the murine central nervous system has either involved the use of open-skull1,2 or ...

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making

睾酮和社会决策，结合行为内分泌和实验经济学：

Behavioral endocrinological research in humans as well as in animals suggests that testosterone plays a key role in social interactions. Studies in ...

Design and Construction of a Cost Effective Headstage for Simultaneous Neural Stimulation and Recording in the Water Maze

同时神经刺激，并记录在水迷宫的设计和构建一个具有成本效益探头

Headstage preamplifiers and source followers are commonly used to study neural activity in behavioral neurophysiology experiments. Available commercial ...

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode

高密度的自由移动，使用基于聚酰亚胺的微电极的小鼠的脑电图记录

Electroencephalogram (EEG) indicates the averaged electrical activity of the neuronal populations on a large-scale level. It is widely utilized as a ...

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

双突触诱发的星形胶质细胞和神经元的反应，在急性海马脑片电生理记录

Astrocytes form together with neurons tripartite synapses, where they integrate and modulate neuronal activity. Indeed, astrocytes sense neuronal inputs ...

A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals

一个程序植入微丝的有组织阵列的单机录音清醒，举止动物

In vivo electrophysiological recordings in the awake, behaving animal provide a powerful method for understanding neural signaling at the single-cell ...

Design and Fabrication of Ultralight Weight, Adjustable Multi-electrode Probes for Electrophysiological Recordings in Mice

设计和超轻重量的制作，可调节的多电极电生理记录小鼠探头

The number of physiological investigations in the mouse, mus musculus, has experienced a recent surge, paralleling the growth in methods of genetic ...

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

自由运动大鼠皮质局部电位、心电图、肌电图和呼吸节律的同步记录

Monitoring the physiological dynamics of the brain and peripheral tissues is necessary for addressing a number of questions about how the brain controls ...

Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset

常见马莫塞的全皮质电皮质阵列的慢性植入

Electrocorticography (ECoG) allows the monitoring of electrical field potentials from the cerebral cortex with high spatiotemporal resolution. Recent ...