Name: design and assembly of an ultra-light motorized microdrive for chronic neural recordings in small animals
Uploaded: 2012-11-08T13:00:00
Description: Watch this Scientific Journal Video about Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals at JoVE.com

Bu çalışma Ester ve Joseph Klingenstein Fonu, McKnight Endowment Fund tarafından desteklenen ve 1R01NS066408-01A1 NINDS edildi.

1. Bileşenleri Genel Bakış <ol><li> Tam bir Microdrive birkaç ana bileşenleri (Şekil 1) oluşur: Sürücü için üstyapı, ince dişli, mil çıkışlı bir motor, elektrotlar taşır ve elektrik bağlantı noktası sağlar ve bir dişli servis olarak hizmet veren bir şasi ve bir Omnetics (veya eşdeğeri) konektörü. </li><li> Şasi, elektrot servis ve elektrot servis tüpler 3D CAD yazılımı (SolidWorks) ile tasarlanmıştır ve yerel hassas makinist tarafından işlendi özel bileşenler...

<ol>
	<li>O'Keefe, J., Dostrovsky, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+hippocampus+as+a+spatial+map.">The hippocampus as a spatial map.</a> Brain Research. 34, 171-175 (1971).</li><li>Pennartz, C. M., Berke, J. D., Graybiel, A. M., Ito, R., Lansink, C. S., van der Meer, M., Redish, A. D., Smith, K. S., Voorn, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Corticostriatal Interactions during Learning, Memory Processing, and Decision. 29, 12831-12838 (2009).</li><li>Kepecs, A., Uchida, N., Zariwala, H., Mainen, Z. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neural+correlates,+computation,+and+behavioural+impact+of+decision+confidence.">Neural correlates, computation, and behavioural impact of decision confidence.</a> Nature. 455, 227-2231 (2008).</li><li>Hahnloser, R. H. R., Kozhevnikov, A. A., Fee, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+ultra-sparse+code+underlies+the+generation+of+neural+sequences+in+a+songbird.">An ultra-sparse code underlies the generation of neural sequences in a songbird.</a> Nature. 419, 65-70 (2002).</li><li>Leonardo, A., Fee, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ensemble+Coding+of+Vocal+Control+in+Birdsong.">Ensemble Coding of Vocal Control in Birdsong.</a> J. Neurosci. 25 (3), 652-661 (2005).</li><li>Yamamoto, J., Wilson, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Large-scale+chronically+implantable+precision+motorized+microdrive+array+for+freely+behaving+animals.">Large-scale chronically implantable precision motorized microdrive array for freely behaving animals.</a> J. Neurophysiol. 100 (4), 2430-2440 (2008).</li><li>Fee, M. S., Leonardo, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Miniature+motorized+microdrive+and+commutator+system+for+chronic+neural+recording+in+small+animals.">Miniature motorized microdrive and commutator system for chronic neural recording in small animals.</a> J. Neurosci. Methods. 112, 83-94 (2001).</li><li>&#214;lveczky, B. P., Otchy, T. M., Goldberg, J. H., Aronov, D., Fee, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Changes+in+the+neural+control+of+a+complex+motor+sequence+during+learning.">Changes in the neural control of a complex motor sequence during learning.</a> J. Neurophys. 106, 386-397 (2011).</li><li>Otchy, T. M., &#214;lveczky, B. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+Sensory+Experience+on+the+Development+and+Maintenance+of+a+Motor+Program+Underlying+a+Complex+Motor+Sequence.">Effects of Sensory Experience on the Development and Maintenance of a Motor Program Underlying a Complex Motor Sequence.</a> Soc. for Neurosci. Abstr. , (2011).</li><li>Goldberg, J. H., Adler, A., Bergman, H., Fee, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Singing-related+neural+activity+distinguishes+two+putative+pallidal+cell+types+in+the+songbird+basal+ganglia:+comparison+to+the+primate+internal+and+external+pallidal+segments.">Singing-related neural activity distinguishes two putative pallidal cell types in the songbird basal ganglia: comparison to the primate internal and external pallidal segments.</a> J. Neurosci. 30 (20), 7088-7098 (2010).</li><li>Goldberg, J. H., Fee, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Singing-related+neural+activity+distinguishes+four+classes+of+putative+striatal+neurons+in+the+songbird+basal+ganglia.">Singing-related neural activity distinguishes four classes of putative striatal neurons in the songbird basal ganglia.</a> J. Neurophys. 103 (4), 2002-2014 (2010).</li><li>Venkatachalam, S., Fee, M. S., Kleinfeld, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Miniature+headstage+with+6-channel+drive+and+vacuum-assisted+microwire+implantation+for+chronic+recording+from+neocortex.">Miniature headstage with 6-channel drive and vacuum-assisted microwire implantation for chronic recording from neocortex.</a> J. Neurosci. Methods. 90, 37-46 (1999).</li><li>Shirvalkar, P. R., Shapiro, M. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Design+and+Construction+of+a+Cost+Effective+Headstage+for+Simultaneous+Neural+Stimulation+and+Recording+in+the+Water+Maze.">Design and Construction of a Cost Effective Headstage for Simultaneous Neural Stimulation and Recording in the Water Maze.</a> J. Vis. Exp. (44), e2155 (2010).</li></ol>

Yapı ile uygun dikkat yalnızca, burada sunulan protokol en az hareket eserler ile yüksek kaliteli kayıtlar yapabilen bir cihaz ile sonuçlanacaktır. Kasadaki mekik uyum kritik önemi ise: çok sıkı ve motor aşırı yüklenmesi riski yüksektir; çok gevşek ve anlamlı hareket artefaktı riski yüksektir. İdeal uygun servis konumu veya gevezelik eğerek dışarı olmadan dişli şaft uzunluğu boyunca yolculuk sağlayacaktır. Kayıt elektrotları seçimi benzer şekilde önemlidir;...

<table border="1">
	<tbody>
 <tr>
 <td>Name of item</td>
 <td>Company</td>
 <td>Catalogue number</td>
 <td>Comments</td>
 </tr>
 <tr>
 <td>Chassis</td>
 <td>custom</td>
 <td></td>
 <td>Cut from PEI</td>
 </tr>
 <tr>
 <td>Electrode Shuttle</td>
 <td>custom</td>
 <td></td>
 <td>Cut from PEI</td>
 </tr>
 <tr>
 <td>Shuttle Tubes</td>
 <td>custom</td>
 <td></td>
 <td>Cut from Stainless Steel</td>
 </tr>
 <tr>
 <td>Connector</td>
 <td>Omnetics</td>
 <td>A7886-001</td>
 <td>Mates to A7877-001</td>
 </tr>
 <tr>
 <td>Motor w/ Gearhead</td>
 <td>Faulhaber</td>
 <td>0206-A-001-B-021-47:1</td>
 <td></td>
 </tr>
 <tr>
 <td>Wire Guide</td>
 <td>Small Parts, Inc</td>
 <td>SWPT-0113-12</td>
 <td></td>
 </tr>
 <tr>
 <td>Electrode Guide</td>
 <td>Small Parts, Inc</td>
 <td>SWPT-0045-12</td>
 <td></td>
 </tr>
 <tr>
 <td>10M&Omega; Pt-Ir electrodes</td>
 <td>Microprobes, Inc</td>
 <td>PI2PT310.0H3</td>
 <td></td>
 </tr>
 <tr>
 <td>Platinum Wire</td>
 <td>A-M Systems</td>
 <td>772000</td>
 <td>For electrode wires</td>
 </tr>
 <tr>
 <td>Silver Wire</td>
 <td>A-M Systems</td>
 <td>786000</td>
 <td>For ground wire</td>
 </tr>
 <tr>
 <td>Tungsten Wire</td>
 <td>A-M Systems</td>
 <td>797000</td>
 <td>For electrode pins</td>
 </tr>
 <tr>
 <td>Transparency</td>
 <td>3M</td>
 <td>AF4300</td>
 <td></td>
 </tr>
 <tr>
 <td>Torr Seal</td>
 <td>Varian</td>
 <td>9530001</td>
 <td></td>
 </tr>
 <tr>
 <td>Kwik-Cast</td>
 <td>WPI</td>
 <td>KWIK-CAST</td>
 <td></td>
 </tr>
 <tr>
 <td>Cyanoacrylate</td>
 <td>Krazy Glue</td>
 <td>KG517</td>
 <td></td>
 </tr>
 <tr>
 <td>Fast-Set Epoxy</td>
 <td>Hardman</td>
 <td>04001</td>
 <td></td>
 </tr>
 <tr>
 <td>Light Mineral Oil</td>
 <td>Sigma</td>
 <td>M5310</td>
 <td></td>
 </tr>
 <tr>
 <td>Chlorine bleach</td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Diagonal cutters</td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Scalpel blade</td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Forceps</td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Drive jig</td>
 <td>custom</td>
 <td></td>
 <td>Epoxy the mating connector to a syringe or stick</td>
 </tr>
 <tr>
 <td>Small Vice</td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
	 </tbody>
 </table>

design and assembly of an ultra-light motorized microdrive for chronic neural recordings in small animals

Kronik serbestçe hareket eden bir hayvana nöronların topluluklarda kayıt yeteneği navigasyon 1 gibi doğal davranışları, karar 2,3 verme çeşitli altta yatan nöral devrelerin fonksiyonu diseksiyon için paha biçilmez bir araç kanıtlanmıştır ve var karmaşık motor dizileri 4 kuşak , 5,6. Hassas işleme gelişmeler örneğin, fare ve ötücü gibi küçük hayvanlar, kronik kayıtları için uygundur hafif cihazların imalatı için izin verdi. Küçük uzaktan kumandalı motorlar ile elektrot konumunu ayarlamak için yeteneği ileri hayvan taşıma azaltarak çeşitli davranışsal bağlamlarda kayıt verimi artmıştır. 6,7 Burada küçük hayvanlarda uzun süreli kronik kayıtları için ultra-hafif motorlu Microdrive inşa etmek için bir protokol açıklar. Bizim tasarım önceki bir yayınlanan sürümü 7 evrimleştiği ve kullanım kolaylığı ve maliyet effecti için adapte edilmişVeness daha pratik ve araştırmacıların geniş bir dizi için erişilebilir olmalıdır. Bu kanıtlanmış tasarım 8,9,10,11 ~ 5 mm aralığında elektrotların ince, uzaktan konumlandırma sağlar ve tamamen monte edildiğinde daha az 750 mg ağırlığında. Hepimiz özel Microdrive bileşenler için 3D CAD çizimleri de dahil olmak üzere, bu sürücüler oluşturmak ve monte etmek için komple protokolü sunarız.

Watch this Scientific Journal Video about Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals at JoVE.com

Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals

Tasarım, imalat ve ultra-hafif motorlu Microdrive montajı açıklanmıştır. Cihaz küçük davranıyor hayvanlarda tek birimler kronik kayıtlar için bir maliyet-etkin ve kolay kullanımlı bir çözüm sağlar.

Küçük Hayvanlar Kronik Sinir Kayıtlar için Ultra-hafif Motorlu Microdrive Tasarımı ve Montajı

The ability to chronically record from populations of neurons in freely behaving animals has proven an invaluable tool for dissecting the function of ...

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