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25.8K Views
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14:48 min
December 26th, 2012
DOI :
10.3791/4322-v
Chapters
0:05
Title
1:53
Preparation of the Mouse Lung
4:02
Enzymatic Digestion of the Lung Tissue
5:40
Preparation of the Lung Cell Suspension
8:02
Antibody Staining for Flow Cytometric Cell-sorting
8:58
Cell-sorting
10:36
Representative Results
14:07
Conclusion
Summary
我々は、フローサイトメトリーのネガティブ選択による一次マウスII型肺胞上皮細胞(AECII)の迅速な単離を記述している。これらAECIIは高い生存率と純度を示し、そのような自己免疫疾患や感染症などの呼吸器疾患におけるそれらの役割に関する機能と分子生物学的研究の広い範囲のために適しています。
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