Name: investigations on alterations of hippocampal circuit function following mild traumatic brain injury
Uploaded: 2012-11-19T12:00:00
Description: Watch this Scientific Journal Video about Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury at JoVE.com

作者想感谢艾略特他的资产阶级用于技术援助。这项工作是由国家机构的健康补助R01HD059288和R01NS069629的。

1。横向闭合性损伤中的作用<ol><li>麻醉的小鼠腹腔注射氯胺酮和甲苯噻嗪的混合物。鼠标的头部，然后准备切口用碘擦洗。 </li><li>执行去骨瓣超过使用3毫米（外径）环锯的右顶叶面积。 </li><li>安全鲁尔-LOC针轮毂（内直径为3毫米）在骨瓣使用cyanoarylate和牙齿的丙烯酸。 </li><li> 24小时后，麻醉吸入异氟醚通过鼠标使用​​。 </li><li>一旦恢复正常的呼吸，但之前鼠标的刺激变得敏感，通过液压冲...

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	<li>Faul, M., Xu, L., Wald, M. M., Coronado, V. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traumatic+Brain+Injury+in+the+United+States:+Emergency+Department+Visits+Hospitalizations+and+Deaths+2002-2006.">Traumatic Brain Injury in the United States: Emergency Department Visits Hospitalizations and Deaths 2002-2006.</a> Centers for Disease Control and Prevention, National Center for Injury Prevention and Control. , (2010).</li><li>McAllister, T. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuropsychiatric+sequelae+of+head+injuries.">Neuropsychiatric sequelae of head injuries.</a> Psychiatr. Clin. North Am. 15, 395-413 (1992).</li><li>Pierce, J. E., Smith, D. H., Trojanowski, J. Q., McIntosh, T. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enduring+cognitive,+neurobehavioral+and+histopathological+changes+persist+for+up+to+one+year+following+severe+experimental+brain+injury+in+rats.">Enduring cognitive, neurobehavioral and histopathological changes persist for up to one year following severe experimental brain injury in rats.</a> NSC. 87, 359-369 (1998).</li><li>Dixon, C. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+fluid+percussion+model+of+experimental+brain+injury+in+the+rat.">A fluid percussion model of experimental brain injury in the rat.</a> J. Neurosurg. 67, 110-119 (1987).</li><li>McIntosh, T. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traumatic+brain+injury+in+the+rat:+characterization+of+a+lateral+fluid-percussion+model.">Traumatic brain injury in the rat: characterization of a lateral fluid-percussion model.</a> Neuroscience. 28, 233-244 (1989).</li><li>Carbonell, W. S., Grady, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regional+and+temporal+characterization+of+neuronal,+glial,+and+axonal+response+after+traumatic+brain+injury+in+the+mouse.">Regional and temporal characterization of neuronal, glial, and axonal response after traumatic brain injury in the mouse.</a> Acta Neuropathol. 98, 396-406 (1999).</li><li>Toth, Z., Hollrigel, G. S., Gorcs, T., Soltesz, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Instantaneous+perturbation+of+dentate+interneuronal+networks+by+a+pressure+wave-transient+delivered+to+the+neocortex.">Instantaneous perturbation of dentate interneuronal networks by a pressure wave-transient delivered to the neocortex.</a> J. Neurosci. 17, 8106-8117 (1997).</li><li>D'Ambrosio, R., Maris, D. O., Grady, M. S., Winn, H. R., Janigro, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selective+loss+of+hippocampal+long-term+potentiation,+but+not+depression,+following+fluid+percussion+injury.">Selective loss of hippocampal long-term potentiation, but not depression, following fluid percussion injury.</a> Brain Res. 786, 64-79 (1998).</li><li>Witgen, B. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regional+hippocampal+alteration+associated+with+cognitive+deficit+following+experimental+brain+injury:+A+systems,+network+and+cellular+evaluation.">Regional hippocampal alteration associated with cognitive deficit following experimental brain injury: A systems, network and cellular evaluation.</a> Neuroscience. 133, 1-15 (2005).</li><li>Schwarzbach, E., Bonislawski, D. P., Xiong, G., Cohen, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanisms+underlying+the+inability+to+induce+area+CA1+LTP+in+the+mouse+after+traumatic+brain+injury.">Mechanisms underlying the inability to induce area CA1 LTP in the mouse after traumatic brain injury.</a> Hippocampus. 16, 541-550 (2006).</li><li>Cole, J. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dietary+branched+chain+amino+acids+ameliorate+injury-induced+cognitive+impairment.">Dietary branched chain amino acids ameliorate injury-induced cognitive impairment.</a> Proceedings of the National Academy of Sciences. 107, 366-371 (2010).</li></ol>

上面列出的每个技术有助于更​​深入的了解所观察到的行为赤字的内在机制造成的。通过结合从每个方法所取得的固有信息，我们能够检查更精确的生物学机制。 测量的fEPSPs是有用的为大，空间定义的区域的神经元量化净突触的功效。它也可以提供信息的一组细胞的潜力，经过突触可塑性，学习和记忆的神经相关的。这是一个非常有用的，因为它是第一点的电生理分析技?...

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investigations on alterations of hippocampal circuit function following mild traumatic brain injury

创伤性脑损伤（TBI）折磨着每年超过170万人在美国，即使是轻微的脑外伤可能会导致持久性神经损伤1。普及和禁用的症状所经历的TBI幸存者，记忆障碍和减少癫痫发作阈值，被认为是介导的海马脑外伤引起的功能障碍2,3。为了演示如何改变伤后在小鼠海马的电路功能造成不利影响的行为，我们采用横向液压冲击伤，常用的TBI动物模型，再现了人类TBI的许多功能，包括神经细胞的损失，胶质细胞增生和离子扰动4 - 6。 在这里，我们展示了一个组合的方法调查TBI对海马功能障碍。我们的方法集成了多种体外生理技术结合起来，与动物行为学和生化分析，以分析在海马-TBI后的变化。我们开始实验的伤害模式与行为分析，评估认知障碍，脑外伤。接下来，我们提供三个不同的体外记录技术：外场势记录，可视化全细胞膜片夹紧，和对电压敏感染料记录。最后，我们展示了次区域的海马-TBI后的神经化学和代谢变化的详细分析，可用于区域解剖的方法。 这些方法已被用于检查修改后的海马电路TBI和探测对方在网络电路功能的变化发生在齿状回的海马CA1次区域（ 见图1）。分析后TBI在各次区域的能力是必不可少的理解基本的机制，促进脑外伤引起的行为和认知ðeficits。 这里列出的多面性的系统，使调查人员推过去的表征现象引起的一种疾病状态（在这种情况下，TBI），并确定负责与TBI所观察到的病理机制。

Watch this Scientific Journal Video about Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury at JoVE.com

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury

一个多层面的方式来调查海马电路的功能变化进行了解释。电技术，以及受伤的协议，行为检测和区域的清扫方法。可以应用这些技术的组合，以类似的方式用于其他大脑区域和科学问题。

调查轻度创伤性脑损伤海马电路功能的改变

Traumatic Brain Injury (TBI) afflicts more than 1.7 million people in the United States each year and even mild TBI can lead to persistent neurological ...

Research

JoVE Journal

Neuroscience

Preparation of Oligomeric &beta;-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Preparation of Oligomeric ÃƒÅ½Ã‚Â²-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

低聚β-淀粉样蛋白的制备 1-42和突触可塑性损伤诱导海马脑片

Impairment of synaptic connections is likely to underlie the subtle amnesic changes occurring at the early stages of Alzheimer s Disease (AD). ...

科研

神经科学

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

侧向液压冲击：创伤性脑损伤小鼠模型

Traumatic brain injury (TBI) research has attained renewed momentum due to the increasing awareness of head injuries, which result in morbidity and ...

A Novel In Vitro Model of Blast Traumatic Brain Injury

A Novel In Vitro Model of Blast Traumatic Brain Injury

一种新的脑外伤体损伤模型

Traumatic brain injury is a leading cause of death and disability in military and civilian populations. Blast traumatic brain injury results from the ...

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

爆炸性神经损伤对压生啮鼠中脑动脉的影响

Though there have been studies on the histopathological and behavioral effects of blast exposure, fewer have been dedicated to blast&#39;s cerebral ...

Investigating Alterations in Caecum Microbiota After Traumatic Brain Injury in Mice

研究小鼠创伤性脑损伤后凯库姆微生物群的改动

Increasing evidence shows that the microbiota-gut-brain axis plays an important role in the pathogenesis of brain diseases. Several studies also ...

Advanced Diffusion Imaging in The Hippocampus of Rats with Mild Traumatic Brain Injury

轻度创伤性脑损伤大鼠海马学的高级扩散成像

Mild traumatic brain injury (mTBI) is the most common type of acquired brain injury. Since patients with traumatic brain injury show a tremendous ...

Low-intensity Blast Wave Model for Preclinical Assessment of Closed-head Mild Traumatic Brain Injury in Rodents

低强度冲击波模型用于啮齿动物闭头轻度创伤性脑损伤的临床前评估

Traumatic brain injury (TBI) is a large-scale public health problem. Mild TBI is the most prevalent form of neurotrauma and accounts for a large number of ...

A Metric Test for Assessing Spatial Working Memory in Adult Rats Following Traumatic Brain Injury

创伤性脑损伤后评估成年大鼠空间工作记忆的指标测试

Impairments to sensory, short-term, and long-term memory are common side effects after traumatic brain injury (TBI). Due to the ethical limitations of ...

Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice

小鼠轻度创伤性脑损伤的电磁控制闭头模型

Highly reproducible animal models of traumatic brain injury (TBI), with well-defined pathologies, are needed for testing therapeutic interventions and ...

Assessing Changes in Synaptic Plasticity Using an Awake Closed-Head Injury Model of Mild Traumatic Brain Injury

使用轻度创伤性脑损伤的清醒闭合性头部损伤模型评估突触可塑性的变化

Mild traumatic brain injuries (mTBIs) are a prevalent health issue in North America. There is increasing pressure to utilize ecologically valid models of ...