Name: investigations on alterations of hippocampal circuit function following mild traumatic brain injury
Uploaded: 2012-11-19T12:00:00
Description: Watch this Scientific Journal Video about Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury at JoVE.com

Forfatterne vil gerne takke Elliot Bourgeois for hans teknisk bistand. Dette arbejde blev finansieret af National Institutes of Health tilskud R01HD059288 og R01NS069629.

1. Lateral Fluid Percussion Skade <ol><li> Bedøve musen under anvendelse af en blanding af ketamin og xylazin givet intraperitonealt. Derefter forberede musens hoved til incision ved hjælp af en jod krat. </li><li> Udføre en kraniektomi over den højre parietale område ved anvendelse af 3 mm (udvendig diameter) trepan. </li><li> Sikker Luer-loc nålenavet (indvendig diameter 3 mm) over kraniektomi hjælp cyanoarylate og dental acrylic. </li><li> 24 timer senere, bedøve musen med isofluran via inhalation. </li><li...

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	<li>Faul, M., Xu, L., Wald, M. M., Coronado, V. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traumatic+Brain+Injury+in+the+United+States:+Emergency+Department+Visits+Hospitalizations+and+Deaths+2002-2006.">Traumatic Brain Injury in the United States: Emergency Department Visits Hospitalizations and Deaths 2002-2006.</a> Centers for Disease Control and Prevention, National Center for Injury Prevention and Control. , (2010).</li><li>McAllister, T. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuropsychiatric+sequelae+of+head+injuries.">Neuropsychiatric sequelae of head injuries.</a> Psychiatr. Clin. North Am. 15, 395-413 (1992).</li><li>Pierce, J. E., Smith, D. H., Trojanowski, J. Q., McIntosh, T. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enduring+cognitive,+neurobehavioral+and+histopathological+changes+persist+for+up+to+one+year+following+severe+experimental+brain+injury+in+rats.">Enduring cognitive, neurobehavioral and histopathological changes persist for up to one year following severe experimental brain injury in rats.</a> NSC. 87, 359-369 (1998).</li><li>Dixon, C. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+fluid+percussion+model+of+experimental+brain+injury+in+the+rat.">A fluid percussion model of experimental brain injury in the rat.</a> J. Neurosurg. 67, 110-119 (1987).</li><li>McIntosh, T. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traumatic+brain+injury+in+the+rat:+characterization+of+a+lateral+fluid-percussion+model.">Traumatic brain injury in the rat: characterization of a lateral fluid-percussion model.</a> Neuroscience. 28, 233-244 (1989).</li><li>Carbonell, W. S., Grady, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regional+and+temporal+characterization+of+neuronal,+glial,+and+axonal+response+after+traumatic+brain+injury+in+the+mouse.">Regional and temporal characterization of neuronal, glial, and axonal response after traumatic brain injury in the mouse.</a> Acta Neuropathol. 98, 396-406 (1999).</li><li>Toth, Z., Hollrigel, G. S., Gorcs, T., Soltesz, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Instantaneous+perturbation+of+dentate+interneuronal+networks+by+a+pressure+wave-transient+delivered+to+the+neocortex.">Instantaneous perturbation of dentate interneuronal networks by a pressure wave-transient delivered to the neocortex.</a> J. Neurosci. 17, 8106-8117 (1997).</li><li>D'Ambrosio, R., Maris, D. O., Grady, M. S., Winn, H. R., Janigro, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selective+loss+of+hippocampal+long-term+potentiation,+but+not+depression,+following+fluid+percussion+injury.">Selective loss of hippocampal long-term potentiation, but not depression, following fluid percussion injury.</a> Brain Res. 786, 64-79 (1998).</li><li>Witgen, B. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regional+hippocampal+alteration+associated+with+cognitive+deficit+following+experimental+brain+injury:+A+systems,+network+and+cellular+evaluation.">Regional hippocampal alteration associated with cognitive deficit following experimental brain injury: A systems, network and cellular evaluation.</a> Neuroscience. 133, 1-15 (2005).</li><li>Schwarzbach, E., Bonislawski, D. P., Xiong, G., Cohen, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanisms+underlying+the+inability+to+induce+area+CA1+LTP+in+the+mouse+after+traumatic+brain+injury.">Mechanisms underlying the inability to induce area CA1 LTP in the mouse after traumatic brain injury.</a> Hippocampus. 16, 541-550 (2006).</li><li>Cole, J. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dietary+branched+chain+amino+acids+ameliorate+injury-induced+cognitive+impairment.">Dietary branched chain amino acids ameliorate injury-induced cognitive impairment.</a> Proceedings of the National Academy of Sciences. 107, 366-371 (2010).</li></ol>

Hver teknik skitseret ovenfor bidrager til større forståelse af den underliggende mekanisme årsag til den konstaterede adfærdsmæssige underskud. Ved at kombinere det unikke oplysninger indhentet ved hver metode, vi er i stand til at undersøge de biologiske mekanismer med mere præcision. Måling fEPSPs er nyttigt til kvantificering af netto-synaptisk virkning af store, rumligt definerede regioner af neuroner. Det kan også give oplysninger om potentialet i en gruppe af celler til at ge...

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investigations on alterations of hippocampal circuit function following mild traumatic brain injury

Traumatisk hjerneskade (TBI) hjemsøger mere end 1,7 millioner mennesker i USA hvert år, og selv mild TBI kan føre til vedvarende neurologiske nedskrivninger 1. To udbredte og invaliderende symptomer opleves af TBI overlevende, hukommelse underskud og en reduktion i krampetærsklen, menes at være medieret af TBI-induceret hippocampus dysfunktion 2,3. For at demonstrere, hvordan ændret hippocampus kredsløbsfunktion skader adfærd efter TBI hos mus, vi beskæftiger lateral væske slagtøj skade, et almindeligt anvendt dyremodel for TBI, der genskaber mange funktioner af human TBI herunder neuronal celletab, gliosis, og ioniske forstyrrelse 4 - 6. Her har vi demonstrere en kombinatorisk metode til at undersøge TBI-induceret hippocampus dysfunktion. Vores tilgang indbefatter adskillige ex vivo fysiologiske teknikker sammen med dyrs adfærd og biokemiske analyser, med henblik på at analyserepost-TBI ændringer i hippocampus. Vi begynder med den eksperimentelle skade paradigme sammen med adfærdsmæssige analyse for at vurdere kognitive handicap efter TBI. Næste, vi har tre forskellige ex vivo optagelse teknikker: ekstracellulære område potentiel optagelse, visualiseret hel-celle patch-fastspænding og spænding følsomt farvestof optagelse. Endelig viser vi en fremgangsmåde til regionalt dissekering subregioner af hippocampus, der kan være nyttig til detaljeret analyse af neurokemiske og metaboliske ændringer efter TBI. Disse fremgangsmåder er blevet anvendt til at undersøge ændringer i hippocampus kredsløb efter TBI og til at probe de modstående ændringer i netværket kredsløbsfunktion, der forekommer i den tandede gyrus og CA1 subregioner af hippocampus (se figur 1). Evnen til at analysere de post-TBI ændringer i hver subregion er nødvendigt for at forstå de underliggende mekanismer, der bidrager til TBI-induceret adfærdsmæssige og kognitive deficits. Den multi-facetteret system, skitseret her giver efterforskerne at skubbe forbi karakterisering af fænomenologi induceret af en sygdomstilstand (i dette tilfælde TBI) og bestemme de mekanismer, der er ansvarlige for den observerede patologi forbundet med TBI.

Watch this Scientific Journal Video about Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury at JoVE.com

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury

En flerstrenget tilgang til at undersøge funktionelle ændringer i hippocampus kredsløb er forklaret. Elektrofysiologiske teknikker er beskrevet sammen med skaden protokol, adfærdsmæssige test og regional dissektionsmetoden. Kombinationen af ​​disse teknikker kan anvendes på lignende måde for andre områder af hjernen og videnskabelige emner.

Undersøgelser om Ændringer af hippocampus Circuit funktion efter Mild traumatisk hjerneskade

Traumatic Brain Injury (TBI) afflicts more than 1.7 million people in the United States each year and even mild TBI can lead to persistent neurological ...

Research

JoVE Journal

Neuroscience

Preparation of Oligomeric &beta;-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Preparation of Oligomeric ÃƒÅ½Ã‚Â²-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Impairment of synaptic connections is likely to underlie the subtle amnesic changes occurring at the early stages of Alzheimer s Disease (AD). ...

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

Traumatic brain injury (TBI) research has attained renewed momentum due to the increasing awareness of head injuries, which result in morbidity and ...

A Novel In Vitro Model of Blast Traumatic Brain Injury

A Novel In Vitro Model of Blast Traumatic Brain Injury

En roman In Vitro Model af Blast traumatisk hjerneskade

Traumatic brain injury is a leading cause of death and disability in military and civilian populations. Blast traumatic brain injury results from the ...

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

Virkningerne af Blast-induceret Neurotrauma på trykisoleret gnaver midt cerebrale arterier

Though there have been studies on the histopathological and behavioral effects of blast exposure, fewer have been dedicated to blast&#39;s cerebral ...

Investigating Alterations in Caecum Microbiota After Traumatic Brain Injury in Mice

Undersøgelse af ændringer i Caecum microbiota efter traumatisk hjerneskade i mus

Increasing evidence shows that the microbiota-gut-brain axis plays an important role in the pathogenesis of brain diseases. Several studies also ...

Advanced Diffusion Imaging in The Hippocampus of Rats with Mild Traumatic Brain Injury

Avanceret diffusion Imaging i hippocampus af rotter med mild traumatisk hjerneskade

Mild traumatic brain injury (mTBI) is the most common type of acquired brain injury. Since patients with traumatic brain injury show a tremendous ...

Low-intensity Blast Wave Model for Preclinical Assessment of Closed-head Mild Traumatic Brain Injury in Rodents

Lavintensitets blastbølgemodel til præklinisk vurdering af mild traumatisk hjerneskade med lukket hoved hos gnavere

Traumatic brain injury (TBI) is a large-scale public health problem. Mild TBI is the most prevalent form of neurotrauma and accounts for a large number of ...

A Metric Test for Assessing Spatial Working Memory in Adult Rats Following Traumatic Brain Injury

En metriske test til vurdering af rumlig arbejdshukommelse hos voksne rotter efter traumatisk hjerneskade

Impairments to sensory, short-term, and long-term memory are common side effects after traumatic brain injury (TBI). Due to the ethical limitations of ...

Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice

Elektromagnetisk kontrolleret lukket hovedmodel af mild traumatisk hjerneskade hos mus

Highly reproducible animal models of traumatic brain injury (TBI), with well-defined pathologies, are needed for testing therapeutic interventions and ...

Assessing Changes in Synaptic Plasticity Using an Awake Closed-Head Injury Model of Mild Traumatic Brain Injury

Vurdering af ændringer i synaptisk plasticitet ved hjælp af en vågen lukket hovedskademodel af mild traumatisk hjerneskade

Mild traumatic brain injuries (mTBIs) are a prevalent health issue in North America. There is increasing pressure to utilize ecologically valid models of ...