Name: investigations on alterations of hippocampal circuit function following mild traumatic brain injury
Uploaded: 2012-11-19T12:00:00
Description: Watch this Scientific Journal Video about Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury at JoVE.com

लेखकों के लिए अपने तकनीकी सहायता के लिए पूंजीपति इलियट धन्यवाद करना चाहते हैं. यह काम स्वास्थ्य R01HD059288 और R01NS069629 अनुदान के राष्ट्रीय संस्थान द्वारा वित्त पोषित किया गया था.

1. पार्श्व द्रव टक्कर चोट <ol><li> Ketamine और xylazine दिया intraperitoneally का एक मिश्रण का उपयोग माउस संज्ञाहीन करना. फिर एक आयोडीन रगडें का उपयोग चीरा के लिए माउस सिर तैयार. </li><li> सही पार्श्विका मिमी 3 (व्यास के बाहर) ईस यंत्र के व?...

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	<li>Faul, M., Xu, L., Wald, M. M., Coronado, V. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traumatic+Brain+Injury+in+the+United+States:+Emergency+Department+Visits+Hospitalizations+and+Deaths+2002-2006.">Traumatic Brain Injury in the United States: Emergency Department Visits Hospitalizations and Deaths 2002-2006.</a> Centers for Disease Control and Prevention, National Center for Injury Prevention and Control. , (2010).</li><li>McAllister, T. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuropsychiatric+sequelae+of+head+injuries.">Neuropsychiatric sequelae of head injuries.</a> Psychiatr. Clin. North Am. 15, 395-413 (1992).</li><li>Pierce, J. E., Smith, D. H., Trojanowski, J. Q., McIntosh, T. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enduring+cognitive,+neurobehavioral+and+histopathological+changes+persist+for+up+to+one+year+following+severe+experimental+brain+injury+in+rats.">Enduring cognitive, neurobehavioral and histopathological changes persist for up to one year following severe experimental brain injury in rats.</a> NSC. 87, 359-369 (1998).</li><li>Dixon, C. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+fluid+percussion+model+of+experimental+brain+injury+in+the+rat.">A fluid percussion model of experimental brain injury in the rat.</a> J. Neurosurg. 67, 110-119 (1987).</li><li>McIntosh, T. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traumatic+brain+injury+in+the+rat:+characterization+of+a+lateral+fluid-percussion+model.">Traumatic brain injury in the rat: characterization of a lateral fluid-percussion model.</a> Neuroscience. 28, 233-244 (1989).</li><li>Carbonell, W. S., Grady, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regional+and+temporal+characterization+of+neuronal,+glial,+and+axonal+response+after+traumatic+brain+injury+in+the+mouse.">Regional and temporal characterization of neuronal, glial, and axonal response after traumatic brain injury in the mouse.</a> Acta Neuropathol. 98, 396-406 (1999).</li><li>Toth, Z., Hollrigel, G. S., Gorcs, T., Soltesz, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Instantaneous+perturbation+of+dentate+interneuronal+networks+by+a+pressure+wave-transient+delivered+to+the+neocortex.">Instantaneous perturbation of dentate interneuronal networks by a pressure wave-transient delivered to the neocortex.</a> J. Neurosci. 17, 8106-8117 (1997).</li><li>D'Ambrosio, R., Maris, D. O., Grady, M. S., Winn, H. R., Janigro, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selective+loss+of+hippocampal+long-term+potentiation,+but+not+depression,+following+fluid+percussion+injury.">Selective loss of hippocampal long-term potentiation, but not depression, following fluid percussion injury.</a> Brain Res. 786, 64-79 (1998).</li><li>Witgen, B. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regional+hippocampal+alteration+associated+with+cognitive+deficit+following+experimental+brain+injury:+A+systems,+network+and+cellular+evaluation.">Regional hippocampal alteration associated with cognitive deficit following experimental brain injury: A systems, network and cellular evaluation.</a> Neuroscience. 133, 1-15 (2005).</li><li>Schwarzbach, E., Bonislawski, D. P., Xiong, G., Cohen, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanisms+underlying+the+inability+to+induce+area+CA1+LTP+in+the+mouse+after+traumatic+brain+injury.">Mechanisms underlying the inability to induce area CA1 LTP in the mouse after traumatic brain injury.</a> Hippocampus. 16, 541-550 (2006).</li><li>Cole, J. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dietary+branched+chain+amino+acids+ameliorate+injury-induced+cognitive+impairment.">Dietary branched chain amino acids ameliorate injury-induced cognitive impairment.</a> Proceedings of the National Academy of Sciences. 107, 366-371 (2010).</li></ol>

प्रत्येक ऊपर उल्लिखित तकनीक अंतर्निहित मनाया व्यवहार घाटे के कारण तंत्र का अधिक से अधिक समझ के लिए योगदान देता है. अद्वितीय प्रत्येक विधि से प्राप्त जानकारी के संयोजन से हम अधिक परिशुद्धता के साथ जैव...

<table border="1"><tbody><tr><td> उपकरणों के नाम </td><td> कंपनी </td><td> सूचीपत्र संख्या </td><td> टिप्पणियां (वैकल्पिक) </td></tr><tr><td> 200B एम्पलीफायर Axopatch </td><td> आणविक उपकरणों </td><td> AXOPATCH 200B </td><td> पैच - दबाना रिग </td></tr><tr><td> Digidata 1322A digitizer </td><td> आणविक उपकरणों </td><td></td><td> पैच - दबाना रिग </td></tr><tr><td> MP-225 micromanipulator </td><td> Sutter </td><td> MP-225 </td><td> पैच - दबाना रिग </td></tr><tr><td> DMLFSA खुर्दबीन </td><td> Leica </td><td></td><td> पैच - दबाना रिग </td></tr><tr><td> 700B एम्पलीफायर Multiclamp </td><td> आणविक उपकरणों </td><td> 700B MULTICLAMP </td><td> बहुउद्देशीय (क्षेत्र)रिग </td></tr><tr><td> Digidata 1440 digitizer </td><td> आणविक उपकरणों </td><td></td><td> Multipurpos रिग (क्षेत्र) </td></tr><tr><td> MPC-200 micromanipulator </td><td> Sutter </td><td> MPC-200 </td><td> बहुउद्देशीय रिग (क्षेत्र) </td></tr><tr><td> BX51WI खुर्दबीन </td><td> ओलिंप </td><td> BX51WI </td><td> बहुउद्देशीय रिग (क्षेत्र) </td></tr><tr><td> 900A प्रवर्धक Axoclamp </td><td> आणविक उपकरणों </td><td> 900A AXOCLAMP </td><td> VSD रिग </td></tr><tr><td> Digidata 1322 digitizer </td><td> आणविक उपकरणों </td><td></td><td> VSD रिग </td></tr><tr><td> Redshirt सीसीडी कैमरा SMQ </td><td> Redshirt </td><td> NCS01 </td><td> VSD रिग </td></tr><tr><td> VT 1200s Vibratome </td><td> Leica </td> &lt;td 14048142066&gt; </td><td></td></tr><tr><td> P-30 इलेक्ट्रोड डांड़ी </td><td> Sutter </td><td> P-30 / पी </td><td></td></tr><tr><td> पूरा protease अवरोध </td><td> Roche </td><td> 11697498001 </td><td></td></tr></tbody></table>

investigations on alterations of hippocampal circuit function following mild traumatic brain injury

Traumatic Brain Injury (TBI) afflicts more than 1.7 million people in the United States each year and even mild TBI can lead to persistent neurological impairments 1. Two pervasive and disabling symptoms experienced by TBI survivors, memory deficits and a reduction in seizure threshold, are thought to be mediated by TBI-induced hippocampal dysfunction 2,3. In order to demonstrate how altered hippocampal circuit function adversely affects behavior after TBI in mice, we employ lateral fluid percussion injury, a commonly used animal model of TBI that recreates many features of human TBI including neuronal cell loss, gliosis, and ionic perturbation 4-6.
	Here we demonstrate a combinatorial method for investigating TBI-induced hippocampal dysfunction. Our approach incorporates multiple ex vivo physiological techniques together with animal behavior and biochemical analysis, in order to analyze post-TBI changes in the hippocampus. We begin with the experimental injury paradigm along with behavioral analysis to assess cognitive disability following TBI. Next, we feature three distinct ex vivo recording techniques: extracellular field potential recording, visualized whole-cell patch-clamping, and voltage sensitive dye recording. Finally, we demonstrate a method for regionally dissecting subregions of the hippocampus that can be useful for detailed analysis of neurochemical and metabolic alterations post-TBI.
	These methods have been used to examine the alterations in hippocampal circuitry following TBI and to probe the opposing changes in network circuit function that occur in the dentate gyrus and CA1 subregions of the hippocampus (see Figure 1). The ability to analyze the post-TBI changes in each subregion is essential to understanding the underlying mechanisms contributing to TBI-induced behavioral and cognitive deficits. 
	The multi-faceted system outlined here allows investigators to push past characterization of phenomenology induced by a disease state (in this case TBI) and determine the mechanisms responsible for the observed pathology associated with TBI.

Watch this Scientific Journal Video about Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury at JoVE.com

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury

Hippocampal circuitry के लिए कार्यात्मक परिवर्तनों की जांच करने के लिए एक बहुआयामी दृष्टिकोण समझाया गया है. Electrophysiological तकनीकों के साथ चोट प्रोटोकॉल, व्यवहार परीक्षण और क्षेत्रीय विच्छेदन विधि के साथ वर्णित हैं. इन तकनीकों के संयोजन अन्य मस्तिष्क क्षेत्रों और वैज्ञानिक सवाल के लिए इसी तरह फैशन में लागू किया जा सकता है.

Hippocampal सर्किट हल्का घाव मस्तिष्क की चोट के बाद समारोह के बदलाव पर जांच

Traumatic Brain Injury (TBI) afflicts more than 1.7 million people in the United States each year and even mild TBI can lead to persistent neurological ...

Research

JoVE Journal

Neuroscience

Preparation of Oligomeric &beta;-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Preparation of Oligomeric ÃƒÅ½Ã‚Â²-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Oligomeric β-amyloid की तैयारी<sub1-42> Hippocampal स्लाइसें और synaptic plasticity हानि की प्रेरण

Impairment of synaptic connections is likely to underlie the subtle amnesic changes occurring at the early stages of Alzheimer s Disease (AD). ...

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

पार्श्व द्रव टक्कर: चूहों में अभिघातजन्य मस्तिष्क चोट के मॉडल

Traumatic brain injury (TBI) research has attained renewed momentum due to the increasing awareness of head injuries, which result in morbidity and ...

A Novel In Vitro Model of Blast Traumatic Brain Injury

A Novel In Vitro Model of Blast Traumatic Brain Injury

एक उपंयास में इन विट्रो मॉडल विस्फोट के दर्दनाक मस्तिष्क चोट

Traumatic brain injury is a leading cause of death and disability in military and civilian populations. Blast traumatic brain injury results from the ...

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

दबाव रोडेंट मध्य मस्तिष्क धमनियों पर ब्लास्ट प्रेरित न्यूरोट्रामा के प्रभाव

Though there have been studies on the histopathological and behavioral effects of blast exposure, fewer have been dedicated to blast&#39;s cerebral ...

Investigating Alterations in Caecum Microbiota After Traumatic Brain Injury in Mice

चूहे में दर्दनाक मस्तिष्क की चोट के बाद केकम माइक्रोबायोटा में परिवर्तन की जांच

Increasing evidence shows that the microbiota-gut-brain axis plays an important role in the pathogenesis of brain diseases. Several studies also ...

Advanced Diffusion Imaging in The Hippocampus of Rats with Mild Traumatic Brain Injury

हल्के अभिघातजन्य मस्तिष्क की चोट के साथ चूहों के हिप्पोकैम्पस में उन्नत प्रसार इमेजिंग

Mild traumatic brain injury (mTBI) is the most common type of acquired brain injury. Since patients with traumatic brain injury show a tremendous ...

Low-intensity Blast Wave Model for Preclinical Assessment of Closed-head Mild Traumatic Brain Injury in Rodents

कृन्तकों में बंद सिर हल्के दर्दनाक मस्तिष्क की चोट के प्रीक्लिनिकल मूल्यांकन के लिए कम तीव्रता वाले ब्लास्ट वेव मॉडल

Traumatic brain injury (TBI) is a large-scale public health problem. Mild TBI is the most prevalent form of neurotrauma and accounts for a large number of ...

A Metric Test for Assessing Spatial Working Memory in Adult Rats Following Traumatic Brain Injury

दर्दनाक मस्तिष्क की चोट के बाद वयस्क चूहों में स्थानिक कार्य स्मृति का आकलन करने के लिए एक मीट्रिक परीक्षण

Impairments to sensory, short-term, and long-term memory are common side effects after traumatic brain injury (TBI). Due to the ethical limitations of ...

Electromagnetic Controlled Closed-Head Model of Mild Traumatic Brain Injury in Mice

चूहों में हल्के दर्दनाक मस्तिष्क की चोट का विद्युत चुम्बकीय नियंत्रित बंद-सिर मॉडल

Highly reproducible animal models of traumatic brain injury (TBI), with well-defined pathologies, are needed for testing therapeutic interventions and ...

Assessing Changes in Synaptic Plasticity Using an Awake Closed-Head Injury Model of Mild Traumatic Brain Injury

हल्के दर्दनाक मस्तिष्क की चोट के एक जागृत बंद-सिर चोट मॉडल का उपयोग करके सिनैप्टिक प्लास्टिसिटी में परिवर्तन का आकलन करना

Mild traumatic brain injuries (mTBIs) are a prevalent health issue in North America. There is increasing pressure to utilize ecologically valid models of ...