Name: a low cost setup for behavioral audiometry in rodents
Uploaded: 2012-10-16T13:00:00
Description: Watch this Scientific Journal Video about A Low Cost Setup for Behavioral Audiometry in Rodents at JoVE.com

这项工作是在大学医院的埃尔兰根 - 纽伦堡大学的跨学科研究中心临床研究（IZKF，项目E7）。

1。设置装配和软件编程<ol><li> D / A卡安装在个人计算机上（ 如 ：6229，美国国家仪器公司NI PCI），并把它连接到突破框（ 如 ：BNC-2110年，美国国家仪器公司），都应该至少支持一个输入和一个输出通道至少各44.1千赫的采样率。 </li><li>将突破盒的输出通过BNC电缆连接到声音放大器（ 如 ：AMP75宽带功率放大器，托马斯·沃尔夫）。 </li><li>安装一个红外摄像头（ 例如 ：?...

<ol>
	<li>Brown, M., Irvine, D. R., Park, V. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Perceptual+learning+on+an+auditory+frequency+discrimination+task+by+cats:+association+with+changes+in+primary+auditory+cortex.">Perceptual learning on an auditory frequency discrimination task by cats: association with changes in primary auditory cortex.</a> Cereb. Cortex. 14, 952-965 (2004).</li><li>Ohl, F. W., Scheich, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Learning-induced+plasticity+in+animal+and+human+auditory+cortex.">Learning-induced plasticity in animal and human auditory cortex.</a> Curr. Opin. Neurobiol. 15, 470-477 (2005).</li><li>Koch, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+neurobiology+of+startle.">The neurobiology of startle.</a> Prog. Neurobiol. 59, 107-128 (1999).</li><li>Larrauri, J., Schmajuk, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prepulse+inhibition+mechanisms+and+cognitive+processes:+a+review+and+model.">Prepulse inhibition mechanisms and cognitive processes: a review and model.</a> EXS. 98, 245-278 (2006).</li><li>Walter, M., Tziridis, K., Ahlf, S., Schulze, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Context+dependent+auditory+thresholds+determined+by+brainstem+audiometry+and+prepulse+inhibition+in+Mongolian+gerbils.">Context dependent auditory thresholds determined by brainstem audiometry and prepulse inhibition in Mongolian gerbils.</a> Open Journal of Acoustics. 2, 34-49 (2012).</li><li>Gaese, B. H., Nowotny, M., Pilz, P. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acoustic+startle+and+prepulse+inhibition+in+the+Mongolian+gerbil.">Acoustic startle and prepulse inhibition in the Mongolian gerbil.</a> Physiol. Behav. 98, 460-466 (2009).</li><li>Fechter, L. D., Sheppard, L., Young, J. S., Zeger, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sensory+threshold+estimation+from+a+continuously+graded+response+produced+by+reflex+modification+audiometry.">Sensory threshold estimation from a continuously graded response produced by reflex modification audiometry.</a> J. Acoust. Soc. Am. 84, 179-185 (1988).</li><li>Turner, J. G., Parrish, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gap+detection+methods+for+assessing+salicylate-induced+tinnitus+and+hyperacusis+in+rats.">Gap detection methods for assessing salicylate-induced tinnitus and hyperacusis in rats.</a> Am. J. Audiol. 17, 185-192 (2008).</li><li>Campeau, S., Davis, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fear+potentiation+of+the+acoustic+startle+reflex+using+noises+of+various+spectral+frequencies+as+conditioned+stimuli.">Fear potentiation of the acoustic startle reflex using noises of various spectral frequencies as conditioned stimuli.</a> Animal Learning &#38; Behavior. 20, 177-186 (1992).</li><li>Young, J. S., Fechter, L. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reflex+inhibition+procedures+for+animal+audiometry:+a+technique+for+assessing+ototoxicity.">Reflex inhibition procedures for animal audiometry: a technique for assessing ototoxicity.</a> J. Acoust. Soc. Am. 73, 1686-1693 (1983).</li><li>Turner, J. G., Brozoski, T. J., Bauer, C. A., Parrish, J. L., Myers, K., Hughes, L. F., Caspary, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gap+detection+deficits+in+rats+with+tinnitus:+a+potential+novel+screening+tool.">Gap detection deficits in rats with tinnitus: a potential novel screening tool.</a> Behav. Neurosci. 120, 188-195 (2006).</li><li>Turner, J., Larsen, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+between+noise+exposure+stimulus+properties+and+tinnitus+in+rats:+Results+of+a+12-month+longitudinal+study.">Relationship between noise exposure stimulus properties and tinnitus in rats: Results of a 12-month longitudinal study.</a> ARO. Abs. 594, (2012).</li><li>Turner, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+measures+of+tinnitus+in+laboratory+animals.">Behavioral measures of tinnitus in laboratory animals.</a> Prog. Brain Res. 166, 147-156 (2007).</li><li>Engineer, N. D., Riley, J. R., Seale, J. D., Vrana, W. A., Shetake, J. A., Shetake, J. A., Sudanagunta, S. P., Borland, M. S., Kilgard, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reversing+pathological+neural+activity+using+targeted+plasticity.">Reversing pathological neural activity using targeted plasticity.</a> Nature. 470, 101-104 (2011).</li></ol>

我们提出了一个基于在啮齿类动物的听力测量声惊吓反应，可用于确定行为听阈（听力图10）和听觉的幻象知觉，如主观耳鸣11前脉冲抑制价格便宜，而且易于制造安装。特别是后者的测量是在最近的几个报告8,12,13,14的焦点，并可以看作是一个先决条件，电调查这种疾病的神经元的机制。使用这种方法可以区分的动物没有发展一个主观的耳鸣声损伤和那些没有这样做一遍?...

a low cost setup for behavioral audiometry in rodents

在听觉动物研究的关键是听证会的基本参数的动物实验中所涉及的主题有准确的信息。这些参数可能是生理性的听觉通路的响应特性， 例如，通过脑干听力检查（BERA）。但是，这些方法允许对应于这些生理参数的听觉知觉，只是间接的和不确定的推断。为了评估的知觉的听力水平，行为的方法，必须使用。知觉的描述，在动物模型中使用的行为方式的一个潜在问题是，这些方法大多涉及某种行为测试的主题之前，可以学习的范例， 如动物可能要学会按杠杆响应于一个声音。由于这些学习范式改变自己的看法1,2，他们因此会影响人们获得任何结果约这些方法，因此必须谨慎解释。例外的是利用反射性反应的范例，因为在这里没有学习的范例之前进行感知测试。一个这样的反射反应是声惊吓反应（ASR），可高度重复性引起意想不到的响亮的声音在天真的动物。这ASR反过来可以影响取决于这个前刺激的感知性的声音之前：声音远高于听觉阈值将完全抑制的振幅的ASR;听起来接近阈值只会稍微抑制ASR。这种现象被称为预脉冲抑制（PPI）的3,4，和PPI的量上的ASR逐渐依赖于预脉冲的感知性。 PPI的ASR因此非常适合以确定行为的听力图天真，非受过训练的动物，以确定听力障碍，甚至以检测出可能在这些主观耳鸣知觉动物。在本文中，我们演示了如何使用这种方法，在啮齿类动物模型（参见文献5），长爪沙鼠（长爪沙鼠），这是一个正常人类的听觉范围内，众所周知模型物种的惊吓反应的研究（ 例如： 6）。

Watch this Scientific Journal Video about A Low Cost Setup for Behavioral Audiometry in Rodents at JoVE.com

A Low Cost Setup for Behavioral Audiometry in Rodents

听到的参数，如听阈，听力有障碍或幻象的看法（主观耳鸣）的行为确定为一种快速，廉价的方法。它使用预脉冲抑制声惊吓响应，并可以容易地实现在个人计算机中，使用一个可编程的AD / DA转换器和压电传感器。

在啮齿动物的行为测听的低成本设置

In auditory animal research it is crucial to have precise information about basic hearing parameters of the animal subjects that are involved in the ...

Research

JoVE Journal

Neuroscience

A General Method for Evaluating Incubation of Sucrose Craving in Rats

评估大鼠蔗糖渴求育成的一个通用方法

For someone on a food-restricted diet, food craving in response to food-paired cues may serve as a key behavioral transition point between abstinence and ...

科研

神经科学

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

移动硅探针的神经行为鼠害的大型记录

A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding of input-output relationships of ...

A Lightweight, Headphones-based System for Manipulating Auditory Feedback in Songbirds

一个轻量级的，耳机为基础的系统操作鸣禽听觉反馈

Experimental manipulations of sensory feedback during complex behavior have provided valuable insights into the computations underlying motor control and ...

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

运行轮活动在啮齿类动物的昼夜节律的记录和分析

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

A Low-cost Method for Analyzing Seizure-like Activity and Movement in Drosophila

A Low-cost Method for Analyzing Seizure-like Activity and Movement in Drosophila

在分析癫痫样活动和运动的一种低成本方法<em&gt;果蝇</em

Video tracking systems have been used widely to analyze Drosophila melanogaster movement and detect various abnormalities in locomotive behavior. While ...

A Human-machine-interface Integrating Low-cost Sensors with a Neuromuscular Electrical Stimulation System for Post-stroke Balance Rehabilitation

人机界面集成的低成本传感器，具有神经肌肉电刺激系统中风后康复平衡

A stroke is caused when an artery carrying blood from heart to an area in the brain bursts or a clot obstructs the blood flow to brain thereby preventing ...

High-density Electroencephalographic Acquisition in a Rodent Model Using Low-cost and Open-source Resources

高密度的脑电图采集在啮齿动物模型使用低成本的开源资源

Advanced electroencephalographic analysis techniques requiring high spatial resolution, including electrical source imaging and measures of network ...

Operant Protocols for Assessing the Cost-benefit Analysis During Reinforced Decision Making by Rodents

啮齿动物强化决策中成本效益分析的操作协议

Reinforcement-guided decision making is the ability to choose between competing courses of action based on the relative value of the benefits and their ...

The Power of Interstimulus Interval for the Assessment of Temporal Processing in Rodents

间刺激间隔对啮齿类动物时间处理评估的力量

Temporal processing deficits have been implicated as a potential elemental dimension of higher-level cognitive processes, commonly observed in ...

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

脑干中的数据采集与分析 在小鼠中引发响应音频测量

Brainstem evoked response audiometry (BERA) is of central relevance in the clinical neurophysiology. As other evoked potential (EP) techniques, such as ...