Name: a low cost setup for behavioral audiometry in rodents
Uploaded: 2012-10-16T13:00:00
Description: Watch this Scientific Journal Video about A Low Cost Setup for Behavioral Audiometry in Rodents at JoVE.com

Dette arbejde blev støttet af Interdisciplinært Center for Klinisk Forskning (IZKF, projekt E7) ved University Hospital fra University of Erlangen-Nürnberg.

1. Opsætning Montering og Software Programmering <ol><li> Installer en D / A kort i en personlig computer (f.eks: NI PCI 6229, National Instruments) og slutte den til en breakout-boks (fx: BNC-2110, National Instruments), bør begge understøtte mindst én indgang og én udgang kanal med en samplingfrekvens på mindst 44,1 kHz hver. </li><li> Forbind udgangen af breakout-box via BNC-kabel til en sund forstærker (f.eks AMP75 bredbånds effektforstærker, Thomas Wulf). </li><li> Installer en...

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Vi præsenterer en billig og nem at bygge setup til audiometriske målinger i gnavere baseret på pre-puls hæmning af akustiske startle reaktioner, der kan bruges til at bestemme adfærdsmæssige høretærskler (= audiogrammer 10) og auditive fantom perceptioner som subjektive tinnitus 11. Især de sidstnævnte målinger er i fokus af flere nylige rapporter 8,12,13,14 og kan ses som en forudsætning for elektrofysiologiske undersøgelser af de neuronale mekanismer bag denne sygdom. Ved ...

a low cost setup for behavioral audiometry in rodents

I auditive dyreforsøg er det afgørende at få nøjagtige oplysninger om grundlæggende høreapparater parametre for animalske emner, der er involveret i forsøgene. Sådanne parametre kan være fysiologiske respons egenskaber for den auditive pathway fx via hjernestammeaudiometri (Bera). Men disse metoder giver kun indirekte og usikker ekstrapoleringer om det auditive percept, der svarer til disse fysiologiske parametre. At vurdere den perceptuelle grad af hørelse, adfærdsmæssige metoder skal anvendes. Et potentielt problem med brugen af adfærdsmæssige metoder til beskrivelse af opfattelsen i dyremodeller er det faktum, at de fleste af disse metoder indebærer en vis form for læring paradigme, før fagene kan behaviorally testes, kan fx dyr nødt til at lære at trykke på en løftestang i respons på en lyd. Da disse learning paradigmer ændre selve sansningen 1,2 de derfor vil påvirke noget resultat om perception opnået meddisse metoder og derfor skal fortolkes med forsigtighed. Undtagelser er paradigmer, der gør brug af refleks respons, fordi her ingen læring paradigmer skal foretages forud for perceptuel testning. En sådan refleks reaktion er den akustiske forskrækkelsesrespons (ASR), der kan meget reproducerbart kan fremkaldes med uventede høje lyde i naive dyr. Denne ASR igen kan påvirkes ved forudgående lyde afhængigt af opfattelsen af ​​denne tidligere stimulus: lyder langt over høretærsklen vil fuldstændig inhiberer amplituden af ​​ASR, lyder tæt på grænsen vil kun svagt inhiberer ASR. Dette fænomen kaldes pre-pulse inhibition (PPI) 3,4, og mængden af PPI i ASR gradvist afhænger af synligheden af præ-puls. PPI af ASR er derfor velegnet til at bestemme adfærdsmæssige audiogrammer hos naive, ikke-uddannede dyr, for at bestemme hørenedsættelse eller endda at opdage mulige subjektive tinnitus perceptioner i dissedyr. I denne rapport demonstrerer anvendelsen af denne metode i en gnavermodel (se også ref. 5), den mongolske hoppemus (Meriones unguiculatus), som er en velkendt model arter for forskrækkelsesrespons forskning inden for det normale menneskelige høreområde (fx 6).

Watch this Scientific Journal Video about A Low Cost Setup for Behavioral Audiometry in Rodents at JoVE.com

A Low Cost Setup for Behavioral Audiometry in Rodents

En hurtig og billig metode til adfærdsmæssige bestemmelse af høre parametre som høretærsklerne, hørenedsættelser eller fantom opfattelser (subjektive tinnitus) er beskrevet. Det bruger pre-pulse inhibition af den akustiske forskrækkelsesrespons og kan nemt implementeres i en personlig computer ved hjælp af en programmerbar AD / DA-konverter og et piezo-sensor.

En Low Cost Opsætning til Behavioral Audiometeret i Gnavere

In auditory animal research it is crucial to have precise information about basic hearing parameters of the animal subjects that are involved in the ...

Research

JoVE Journal

Neuroscience

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