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DOI :
10.3791/4448-v
October 14th, 2012
Chapters
0:05
Title
1:27
Assembly of the Microfluidic Culture Chamber (MCP)
2:43
Preparation of Neuronal Culture
3:33
Induction of Neuronal Axons in Assembled MCP
4:48
A Compartmentalized Co-culture System: Adding Cultured Astrocytes
6:15
Results: Axon-induced GLT1 Promoter Activation in Astrocytes
9:07
Conclusion
本研究介绍了一种新的神经元轴突和神经胶质细胞共培养(ASTRO)平台的程序。在此共培养体系中的一个单一的轴突(及单一的神经胶质细胞)之间的直接相互作用,操纵变得可行,允许机械分析的相互神经胶质信令。
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