Name: imaging analysis of neuron to glia interaction in microfluidic culture platform (mcp)-based neuronal axon and glia co-culture system
Uploaded: 2012-10-14T14:00:00
Description: Watch this Scientific Journal Video about Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform MCP-based Neuronal Axon and Glia Co-culture System at JoVE.com

हम बीएसी GLT1 EGFP चूहों और GLT1 एंटीबॉडी प्रदान करने के लिए डॉ. Jeffrey रोथस्टीन धन्यवाद देना चाहूंगा, तंत्रिका विज्ञान अनुसंधान के लिए Tufts केंद्र (P30 NIH NS047243, PI, रोब जैक्सन) मूल्यवान कोर की सुविधा प्रदान करने के लिए नए शिक्षकों की भर्ती के अनुदान (P30 5P30NS069254-02 NIH , PI, फिल Haydon Tufts तंत्रिका विज्ञान विभाग में).

1. Microfluidic संस्कृति चैंबर के विधानसभा (MCP) <ol><li> MCP (1 चित्रा) खुला कक्षों 4 कोशिकाओं के विभिन्न प्रकार के compartmented संस्कृतियों के लिए तैयार हैं. यह आम तौर पर दो डिब्बों कि केंद्रीय चैनल (व्यास में 3 सुक्ष्मम...

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MCP आधारित न्यूरॉन और astrocytes सह संस्कृति प्रणाली केवल axons केंद्रीय चैनल के पास अनुमति और astroglial कोशिकाओं के साथ बातचीत के द्वारा अस्थिकणिका संकेत दे रास्ते विस्तृत न्यूरॉन के विच्छेदन की अनुमति देता है. यह सह ...

<table border="1"><tbody><tr><td> भ्रूण गोजातीय सीरम </td><td> Hyclone </td><td> SH30070.03 </td><td> न्यूरॉन cutlure मध्यम के लिए न्यूरॉन चढ़ाना के लिए </td></tr><tr><td> भ्रूण गोजातीय सीरम </td><td> सिग्मा Aldrich </td><td> F4135 </td><td> astrocyte संस्कृति मध्यम के लिए </td></tr><tr><td> Glial व्युत्पन्न तंत्रिका कारक </td><td> आर एंड डी सिस्टम </td><td> 212-GD </td><td> चैम्बर के न्यूरॉन ओर 10-20 एनजी / एमएल लागू </td></tr><tr><td> Dulbecco संशोधित ईगल मध्यम उच्च ग्लूकोज </td><td> सिग्मा Aldrich </td><td> 11995 </td><td></td></tr><tr><td> 70 मिमी सेल झरनी </td><td> BD फाल्कन </td><td> 352350 </td><td></td></tr><tr><td> बाँझ गिलास नीचे पकवान </td><td> मटेक निगम </td><td></td><td></td></tr><tr><td> Microfluidic संस्कृति प्लेटफार्मों </td><td> Xona Microfluidics LLC </td><td> SND150 </td><td></td></tr><tr><td> संस्कृति की थाली के 6 कुओं </td&gt; <td> Cellstar </td><td> +६५७ +१६० </td><td></td></tr><tr><td></td><td></td><td></td><td> न्यूरॉन संस्कृति के माध्यम <ul><li> Neurobasal मध्यम </li><li> 2% B27 Neurobasal पूरक </li><li> 2 मिमी 1% 100x GlutaMAX जोड़कर ग्लूटामेट </li><li> 1% पेनिसिलिन - streptomysin </li></ul></td></tr><tr><td></td><td></td><td></td><td> न्यूरॉन चढ़ाना सेल के लिए संस्कृति के माध्यम <ul><li> Neurobasal मध्यम </li><li> 2% B27 Neurobasal पूरक </li><li> 2 मिमी 1% 100x GlutaMAX जोड़कर ग्लूटामेट </li><li> 1% पेनिसिलिन - streptomysin </li><li> 5% भ्रूण गोजातीय सीरम SH30070.03 </li></ul></td></tr><tr><td></td><td></td><td></td><td> Astrocyte संस्कृति के माध्यम <ul><li> Dulbecco संशोधित eagle मध्यम उच्च ग्लूकोज </li><li> 10% भ्रूण गोजातीय सीरम F4135 </li><li> 1% पेनिसिलिन - streptomysin </li></ul></td></tr><tr><td></td><td></td><td></td><td> तालिका 1. Microfluidic संस्कृति मंच आधारित neuronal अक्षतंतु और glia प्रणाली सह संस्कृति में प्रयुक्त सामग्री. </td></tr></tbody></table>

imaging analysis of neuron to glia interaction in microfluidic culture platform (mcp)-based neuronal axon and glia co-culture system

Proper neuron to glia interaction is critical to physiological function of the central nervous system (CNS). This bidirectional communication is sophisticatedly mediated by specific signaling pathways between neuron and glia1,2 . Identification and characterization of these signaling pathways is essential to the understanding of how neuron to glia interaction shapes CNS physiology. Previously, neuron and glia mixed cultures have been widely utilized for testing and characterizing signaling pathways between neuron and glia. What we have learned from these preparations and other in vivo tools, however, has suggested that mutual signaling between neuron and glia often occurred in specific compartments within neurons (i.e., axon, dendrite, or soma)3. This makes it important to develop a new culture system that allows separation of neuronal compartments and specifically examines the interaction between glia and neuronal axons/dendrites. In addition, the conventional mixed culture system is not capable of differentiating the soluble factors and direct membrane contact signals between neuron and glia. Furthermore, the large quantity of neurons and glial cells in the conventional co-culture system lacks the resolution necessary to observe the interaction between a single axon and a glial cell.
	In this study, we describe a novel axon and glia co-culture system with the use of a microfluidic culture platform (MCP). In this co-culture system, neurons and glial cells are cultured in two separate chambers that are connected through multiple central channels. In this microfluidic culture platform, only neuronal processes (especially axons) can enter the glial side through the central channels. In combination with powerful fluorescent protein labeling, this system allows direct examination of signaling pathways between axonal/dendritic and glial interactions, such as axon-mediated transcriptional regulation in glia, glia-mediated receptor trafficking in neuronal terminals, and glia-mediated axon growth. The narrow diameter of the chamber also significantly prohibits the flow of the neuron-enriched medium into the glial chamber, facilitating probing of the direct membrane-protein interaction between axons/dendrites and glial surfaces.

Astrocytes में अक्षतंतु प्रेरित GLT1 प्रमोटर सक्रियण के समय चूक इमेजिंग विश्लेषण compartmented न्यूरॉन और astrocyte प्रणाली सह संस्कृति केवल neuronal प्रक्रियाओं, विशेष रूप से axons, चुनिंदा astrocytes के साथ बातचीत करने की अनुमत...

Watch this Scientific Journal Video about Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform MCP-based Neuronal Axon and Glia Co-culture System at JoVE.com

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform MCP-based Neuronal Axon and Glia Co-culture System

इस अध्ययन एक उपन्यास neuronal अक्षतंतु और glia (खगोल) के सह - संस्कृति मंच की स्थापना की प्रक्रिया का वर्णन करता है. इस प्रणाली में सह संस्कृति, एक एकल अक्षतंतु (और एक glial सेल) के बीच सीधी बातचीत के हेरफेर संभव हो जाता है, glial संकेत आपसी न्यूरॉन के यंत्रवत विश्लेषण की अनुमति देता है.

इमेजिंग न्यूरॉन Microfluidic संस्कृति प्लेटफार्म में Glia सहभागिता (MCP) आधारित Neuronal Axon और Glia प्रणाली सह संस्कृति विश्लेषण

Proper  neuron to glia interaction is critical to physiological function of the central  nervous system (CNS). This bidirectional communication is ...

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Neuroscience

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

प्राथमिक चूहा cortical न्यूरॉन्स और Glia Bilaminar सह संस्कृति

This video will guide you through the process of culturing rat cortical neurons in the presence of a glial feeder layer, a system known as a bilaminar or ...

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Microfluidic सह संस्कृति उपकरणों का प्रयोग करना टूथ रोगाणु INNERVATION विकास का विश्लेषण

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संयुक्त तरीके का उपयोग एक्जॉन शाखाओं में बंटी और Neuronal Morphogenesis के दौरान के प्लाज्मा झिल्ली प्रसव भूमिका को परिभाषित करने के लिए

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अप्रत्यक्ष न्यूरॉन astrocyte coculture परख: एक<em&gt; इन विट्रो</em&gt; न्यूरॉन glia बातचीत की विस्तृत जांच के लिए सेट-अप

Proper neuronal development and function is the prerequisite of the developing and the adult brain. However, the mechanisms underlying the highly ...

M&#252;ller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

म्यूलर Glia सेल सक्रियकरण में एक लेजर प्रेरित रेटिना अध-पतन और Zebrafish में मॉडल पुनर्जनन

A fascinating difference between teleost and mammals is the lifelong potential of the teleost retina for retinal neurogenesis and regeneration after ...

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माइक्रोफ्लूइडिक चैम्बर्स सिस्टम का उपयोग करके मोटर न्यूरॉन संस्कृतियों में ऑर्गेनेल्स का एक्सोनल परिवहन

Motor neurons (MNs) are highly polarized cells with very long axons. Axonal transport is a crucial mechanism for MN health, contributing to neuronal ...

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

वयस्क चूहा मूत्राशय से प्राथमिक न्यूरॉन्स और ग्लिया की अलगाव और संस्कृति

The lower urinary tract has two main functions, namely, periodic urine storage and micturition; these functions are mediated through central and ...

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ब्रेन सर्किटरी के गैर-इनवेसिव वियोग के लिए लक्षित न्यूरोनल चोट

Surgical intervention can be quite effective for treating certain types of medically intractable neurological diseases. This approach is particularly ...

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The methods presented here demonstrate laboratory procedures for the dissection of four different regions of the central nervous system (CNS) from murine ...

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इस्केमिया में न्यूरॉन-ग्लिया इंटरैक्शन की भूमिका का अध्ययन करने के लिए एक सेल संस्कृति मॉडल

Ischemic stroke is a clinical condition characterized by hypoperfusion of brain tissue, leading to oxygen and glucose deprivation, and the consequent ...