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14.8K Views
•
09:34 min
October 14th, 2012
DOI :
10.3791/4448-v
Chapters
0:05
Title
1:27
Assembly of the Microfluidic Culture Chamber (MCP)
2:43
Preparation of Neuronal Culture
3:33
Induction of Neuronal Axons in Assembled MCP
4:48
A Compartmentalized Co-culture System: Adding Cultured Astrocytes
6:15
Results: Axon-induced GLT1 Promoter Activation in Astrocytes
9:07
Conclusion
Summary
この研究では、神経細胞の軸索と小説(ASTRO)グリア共培養プラットフォームをセットアップする手順について説明します。この共培養系では、単一の軸索(と単一神経膠細胞)との間の直接的な相互作用の操作は、グリアシグナリング相互ニューロンのメカニズム解析を可能にすることが可能となる。
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