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16:33 min
February 18th, 2013
DOI :
10.3791/50031-v
Chapters
0:05
Title
2:08
Extraction of Target Protein from Cultured Primary Hippocampal Neurons
5:54
Acyl-Biotin Exchange: Hydroxylamine (HAM) Cleavage
9:17
Elution of Biotin-conjugated Target Protein and SDS-PAGE
10:46
Western Blotting and Detection of Palmitoylation of Target Protein
12:16
Results: Detecting Palmitoylation of a Neuronal Protein
16:07
Conclusion
Summary
棕榈酸与蛋白质的可逆加成是一个重要的调节细胞内蛋白质的活动。许多突触蛋白被棕榈酰化的神经元中,这是一个特别令人感兴趣。我们利用一个简单的检测棕榈酰化蛋白在培养的神经元,这可适于多种细胞类型和组织的生化方法。
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