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26.9K Views
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10:00 min
December 19th, 2012
DOI :
10.3791/50049-v
Chapters
0:05
Title
2:03
Cell Culture
2:51
Neutral Comet Assay
5:06
Alkaline Comet Assay
6:09
Fixing and Staining Cells
6:56
Image Acquisition and Analysis
7:31
Results: Representative Comet Assays Assessing DNA Damage in Hippocampal Neurons
9:06
Conclusion
Summary
コメットアッセイは、アルカリに不安定なサイトや海馬ニューロンを含むすべての細胞のDNAにDNA-DNA/DNA-proteinクロスリンクを含むシングルと二重鎖切断を検出する効率的な方法です。この方法は、DNA損傷の量の違いに起因する電界中のDNAの差のマイグレーションを活用しています。
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