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Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

18.1K Views

09:57 min

January 30th, 2013

January 30th, 2013

18,127 Views

0:05

Title

2:15

Collection and Fixation of Drosophila Embryos

4:44

Post-fixation Processing

5:58

Hybridization and Post-hybridization Washes

8:14

FISH Showing Subcellular Localization of mRNA in Drosophila Embryos

9:06

Conclusion

Here we describe a whole-mount fluorescent in situ hybridization (FISH) protocol for determining the expression and localization properties of RNAs expressed during embryogenesis in the fruit fly, Drosophila melanogaster.

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