Name: measurement of lifespan in drosophila melanogaster
Uploaded: 2013-01-07T13:00:00
Description: Watch this Scientific Journal Video about Measurement of Lifespan in Drosophila melanogaster at JoVE.com

इस काम एलिसन मेडिकल (SDP, फाउंडेशन से धन के द्वारा समर्थित किया गया <a href="http://www.ellisonfoundation.org/index.jsp" target="_blank">http://www.ellisonfoundation.org/index.jsp</a> ), NIH K01AG031917 (NJL, <a href="http://www.nih.gov" target="_blank">http://www.nih.gov/</a> ), 5T32GM007315-35 NIH (जेआर) और NIH R01AG030593 (SDP). यह काम P30 एजी 013,283 (उम्र बढ़ने के राष्ट्रीय संस्थान द्वारा वित्त पोषित उम्र बढ़ने के जीवविज्ञान में उत्कृष्टता के नाथन शॉक केंद्र के ड्रोसोफिला एजिंग कोर (डीएसी) के संसाधनों का उपयोग <a href="http://www.nih.gov" target="_blank">http://www.nih.gov/</a> ). लेखकों के लिए उपयोगी और पांडुलिपि के महत्वपूर्ण पढ़ने के लिए विचार - विमर्श के लिए विशेष रूप से ब्रायन चुंग Pletcher प्रयोगशाला का शुक्रिया अदा करना चाहते हैं. हम डेटा संग्रह के साथ सहायता के लिए निक आशेर और कॅथ्रीन Borowicz स्वीकार करना चाहते हैं.

हम प्रयोगात्मक खाद्य पदार्थ, खमीर पेस्ट, और अंगूर अगर प्लेट कि 4 डिग्री सेल्सियस पर प्रोटोकॉल में दिखाई देते हैं भंडारण और उन्हें 1-2 महीने के भीतर के रूप में लंबे समय के रूप में ढालना और सूखापन दोनों लार्...

<ol>
	<li>Toivonen, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=No+influence+of+Indy+on+lifespan+in+Drosophila+after+correction+for+genetic+and+cytoplasmic+background+effects.">No influence of Indy on lifespan in Drosophila after correction for genetic and cytoplasmic background effects.</a> PLoS Genet. 3, e95 (2007).</li><li>Spencer, C. C., Howell, C. E., Wright, A. R., Promislow, D. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Testing+an+'aging+gene'+in+long-lived+drosophila+strains:+increased+longevity+depends+on+sex+and+genetic+background.">Testing an 'aging gene' in long-lived drosophila strains: increased longevity depends on sex and genetic background.</a> Aging Cell. 2, 123-130 (2003).</li><li>Burnett, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Absence+of+effects+of+Sir2+overexpression+on+lifespan+in+C.+elegans+and+Drosophila.">Absence of effects of Sir2 overexpression on lifespan in C. elegans and Drosophila.</a> Nature. 477, 482-485 (2011).</li><li>Bokov, A. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Does+reduced+IGF-1R+signaling+in+Igf1r+/-+mice+alter+aging?.">Does reduced IGF-1R signaling in Igf1r+/- mice alter aging?.</a> PLoS One. 6, e26891 (2011).</li><li>Lewis, E. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+standard+food+medium.">A new standard food medium.</a> Drosophila Information Service. 34, 117-118 (1960).</li><li>Skorupa, D. A., Dervisefendic, A., Zwiener, J., Pletcher, S. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dietary+composition+specifies+consumption,+obesity,+and+lifespan+in+Drosophila+melanogaster.">Dietary composition specifies consumption, obesity, and lifespan in Drosophila melanogaster.</a> Aging Cell. 7, 478-490 (2008).</li><li>Rera, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modulation+of+longevity+and+tissue+homeostasis+by+the+Drosophila+PGC-1+homolog.">Modulation of longevity and tissue homeostasis by the Drosophila PGC-1 homolog.</a> Cell Metab. 14, 623-634 (2011).</li><li>Kaplan, E. L., Meier, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nonparametric+Estimation+from+Incomplete+Observations.">Nonparametric Estimation from Incomplete Observations.</a> Journal of the American Statistical Association. 53, 457-481 (1958).</li><li>Pletcher, S. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mitigating+the+Tithonus+Error:+Genetic+Analysis+of+Mortality+Phenotypes.">Mitigating the Tithonus Error: Genetic Analysis of Mortality Phenotypes.</a> Sci. Aging Knowl. Environ. 2002, pe14 (2002).</li><li>Pletcher, S. D., Khazaeli, A. A., Curtsinger, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Why+do+life+spans+differ?+Partitioning+mean+longevity+differences+in+terms+of+age-specific+mortality+parameters.">Why do life spans differ? Partitioning mean longevity differences in terms of age-specific mortality parameters.</a> J. Gerontol. A Biol. Sci. Med. Sci. 55, 381-389 (2000).</li><li>Promislow, , Tatar, , Pletcher, , Carey, <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Below-threshold+mortality:+implications+for+studies+in+evolution,+ecology+and+demography.">Below-threshold mortality: implications for studies in evolution, ecology and demography.</a> Journal of Evolutionary Biology. 12, 314-328 (1999).</li><li>Pletcher, <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Model+fitting+and+hypothesis+testing+for+age-specific+mortality+data.">Model fitting and hypothesis testing for age-specific mortality data.</a> Journal of Evolutionary Biology. 12, 430-439 (1999).</li><li>Partridge, L., Gems, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Benchmarks+for+ageing+studies.">Benchmarks for ageing studies.</a> Nature. 450, 165-167 (2007).</li><li>Roman, G., Endo, K., Zong, L., Davis, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=P[Switch],+a+system+for+spatial+and+temporal+control+of+gene+expression+in+Drosophila+melanogaster.">P[Switch], a system for spatial and temporal control of gene expression in Drosophila melanogaster.</a> Proceedings of the National Academy of Sciences of the United States of America. 98, 12602-12607 (2001).</li><li>Ford, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Alteration+of+Drosophila+life+span+using+conditional,+tissue-specific+expression+of+transgenes+triggered+by+doxycyline+or+RU486/Mifepristone.">Alteration of Drosophila life span using conditional, tissue-specific expression of transgenes triggered by doxycyline or RU486/Mifepristone.</a> Exp. Gerontol. 42, 483-497 (2007).</li><li>Priest, N. K., Mackowiak, B., Promislow, D. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+parental+age+effects+on+the+evolution+of+aging.">The role of parental age effects on the evolution of aging.</a> Evolution. 56, 927-935 (2002).</li><li>Smith, E. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Feeding+Drosophila+a+biotin-deficient+diet+for+multiple+generations+increases+stress+resistance+and+lifespan+and+alters+gene+expression+and+histone+biotinylation+patterns.">Feeding Drosophila a biotin-deficient diet for multiple generations increases stress resistance and lifespan and alters gene expression and histone biotinylation patterns.</a> J. Nutr. 137, 2006-2012 (2007).</li><li>Sorensen, J. G., Loeschcke, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Larval+crowding+in+Drosophila+melanogaster+induces+Hsp70+expression,+and+leads+to+increased+adult+longevity+and+adult+thermal+stress+resistance.">Larval crowding in Drosophila melanogaster induces Hsp70 expression, and leads to increased adult longevity and adult thermal stress resistance.</a> J. Insect Physiol. 47, 1301-1307 (2001).</li><li>Bass, T. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimization+of+dietary+restriction+protocols+in+Drosophila.">Optimization of dietary restriction protocols in Drosophila.</a> J. Gerontol. A Biol. Sci. Med. Sci. 62, 1071-1081 (2007).</li><li>Miquel, J., Lundgren, P. R., Bensch, K. G., Atlan, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+temperature+on+the+life+span,+vitality+and+fine+structure+of+Drosophila+melanogaster.">Effects of temperature on the life span, vitality and fine structure of Drosophila melanogaster.</a> Mechanisms of Ageing and Development. 5, 347-370 (1976).</li><li>Pittendrigh, C. S., Minis, D. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Circadian+systems:+longevity+as+a+function+of+circadian+resonance+in+Drosophila+melanogaster.">Circadian systems: longevity as a function of circadian resonance in Drosophila melanogaster.</a> Proceedings of the National Academy of Sciences of the United States of America. 69, 1537-1539 (1972).</li><li>Joshi, A., Mueller, L. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adult+crowding+effects+on+longevity+in+Drosophila+melanogaster:+Increase+in+age-dependent+mortality.">Adult crowding effects on longevity in Drosophila melanogaster: Increase in age-dependent mortality.</a> Current Science. 72, 255-260 (1997).</li><li>Ja, W. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prandiology+of+Drosophila+and+the+CAFE+assay.">Prandiology of Drosophila and the CAFE assay.</a> Proceedings of the National Academy of Sciences of the United States of America. 104, 8253-8256 (2007).</li><li>Lee, K. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lifespan+and+reproduction+in+Drosophila:+New+insights+from+nutritional+geometry.">Lifespan and reproduction in Drosophila: New insights from nutritional geometry.</a> Proceedings of the National Academy of Sciences of the United States of America. 105, 2498-2503 (2008).</li><li>Gargano, J. W., Martin, I., Bhandari, P., Grotewiel, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+iterative+negative+geotaxis+(RING):+a+new+method+for+assessing+age-related+locomotor+decline+in+Drosophila.">Rapid iterative negative geotaxis (RING): a new method for assessing age-related locomotor decline in Drosophila.</a> Experimental gerontology. 40, 386-395 (2005).</li></ol>

यहाँ प्रस्तुत प्रोटोकॉल ड्रोसोफिला में वयस्क दीर्घायु की प्रतिलिपि प्रस्तुत करने योग्य माप है कि आनुवंशिक, औषधीय, और पर्यावरण के हस्तक्षेप के मूल्यांकन के लिए अनुकूल है के उत्पादन के लिए एक विधि ?...

<table border="1"><tbody><tr><td> अभिकर्मक के नाम </td><td> कंपनी </td><td> सूचीपत्र संख्या </td><td> टिप्पणियां (वैकल्पिक) </td></tr><tr><td> सक्रिय सूखी खमीर </td><td> Fleishmann खमीर </td><td> 2192 </td><td></td></tr><tr><td> अंगूर अगर पाउडर Premix </td><td> Genesee वैज्ञानिक </td><td> 47-102 </td><td></td></tr><tr><td> बड़े भ्रूण संग्रह पिंजरों </td><td> Genesee वैज्ञानिक </td><td> 59-101 </td><td></td></tr><tr><td> बड़े रिप्लेसमेंट समाप्ति कैप्स </td><td> Genesee वैज्ञानिक </td><td> 59-103 </td><td></td></tr><tr><td> 6 ऑउंस स्क्वायर नीचे की बोतलें, polypropylene </td><td> Genesee वैज्ञानिक </td><td> 32-130 </td><td></td></tr><tr><td> स्टॉक की बोतल के लिए Flugs Closures </td><td> Genesee वैज्ञानिक </td><td> 49-100 </td><td></td></tr><tr><td> ड्रोसोफिलाशीशियों, वाइड Polystrene, </td><td> Genesee वैज्ञानिक </td><td> 32-117 </td><td></td></tr><tr><td> वाइड शीशियों के लिए क्लोजर Flugs </td><td> Genesee वैज्ञानिक </td><td> 49-101 </td><td></td></tr><tr><td> वाइड छिद्र Aardvark pipet टिप्स, 200 उल </td><td> Denville वैज्ञानिक </td><td> P1105-CP </td><td></td></tr><tr><td> Flystuff Flypad, मानक आकार </td><td> Genesee वैज्ञानिक </td><td> 59-114 </td><td></td></tr><tr><td> BD फाल्कन 15 मिलीलीटर शंक्वाकार अपकेंद्रित्र ट्यूबों </td><td> फिशर साइंटिफिक </td><td> 14 ९५९-70C </td><td></td></tr><tr><td> Fisherbrand स्पष्ट Lids के साथ पेट्री डिश, रिज उठाया, 100 आयुध डिपो x 15 मिमी एच; </td><td> फिशर साइंटिफिक </td><td> 08-757-12 </td><td></td></tr><tr><td> Kimax * Colorware हज़ार मिलीलीटर पीला बोतल </td><td> फिशर साइंटिफिक </td><td> 10-200-47 </td><td></td></tr><tr><td> पीबीएस पीएच 7.4 10x </td><td> मेंvitrogen </td><td> 70011044 </td><td></td></tr><tr><td> Gelidium अग्रवाल </td><td> Mooragar </td><td> n / a </td><td></td></tr><tr><td> शराब बनानेवाला है खमीर </td><td> सांसद BIOMEDICALS </td><td> 0290331280 </td><td></td></tr><tr><td> दानेदार चीनी </td><td> क्रोजर </td><td> n / a </td><td></td></tr><tr><td> Tegosept </td><td> Genesee वैज्ञानिक </td><td> 20-266 </td><td> खाद्य परिरक्षक उड़ना </td></tr><tr><td> Propionic एसिड, 99% </td><td> Acros ऑर्गेनिक्स </td><td> 149300025 </td><td> खाद्य परिरक्षक उड़ना </td></tr><tr><td> केनामाइसिन सल्फेट </td><td> ISC BioExpress </td><td> 0408-10G </td><td></td></tr><tr><td> टेट्रासाइक्लिन एचसीएल </td><td> VWR </td><td> 80058-724 </td><td></td></tr></tbody></table>

measurement of lifespan in drosophila melanogaster

Aging is a phenomenon that results in steady physiological deterioration in nearly all organisms in which it has been examined, leading to reduced physical performance and increased risk of disease. Individual aging is manifest at the population level as an increase in age-dependent mortality, which is often measured in the laboratory by observing lifespan in large cohorts of age-matched individuals. Experiments that seek to quantify the extent to which genetic or environmental manipulations impact lifespan in simple model organisms have been remarkably successful for understanding the aspects of aging that are conserved across taxa and for inspiring new strategies for extending lifespan and preventing age-associated disease in mammals.
The vinegar fly, Drosophila melanogaster, is an attractive model organism for studying the mechanisms of aging due to its relatively short lifespan, convenient husbandry, and facile genetics. However, demographic measures of aging, including age-specific survival and mortality, are extraordinarily susceptible to even minor variations in experimental design and environment, and the maintenance of strict laboratory practices for the duration of aging experiments is required. These considerations, together with the need to practice careful control of genetic background, are essential for generating robust measurements. Indeed, there are many notable controversies surrounding inference from longevity experiments in yeast, worms, flies and mice that have been traced to environmental or genetic artifacts1-4. In this protocol, we describe a set of procedures that have been optimized over many years of measuring longevity in Drosophila using laboratory vials. We also describe the use of the dLife software, which was developed by our laboratory and is available for download (<a href="http://sitemaker.umich.edu/pletcherlab/software">http://sitemaker.umich.edu/pletcherlab/software</a>). dLife accelerates throughput and promotes good practices by incorporating optimal experimental design, simplifying fly handling and data collection, and standardizing data analysis. We will also discuss the many potential pitfalls in the design, collection, and interpretation of lifespan data, and we provide steps to avoid these dangers.

प्रोटोकॉल का एक सरलीकृत योजना 1 चित्रा, जहां महत्वपूर्ण कदम को रेखांकित कर रहे हैं में प्रस्तुत किया है. प्रोटोकॉल के तुल्यकालन हिस्सा विभिन्न assays कि उम्र से मिलान वयस्क मक्खियों की आवश्यकता के ल...

Watch this Scientific Journal Video about Measurement of Lifespan in Drosophila melanogaster at JoVE.com

Measurement of Lifespan in Drosophila melanogaster

ड्रोसोफिला मेलानोगास्टर दीर्घायु विनियमन के आणविक आधार की खोज के लिए एक शक्तिशाली मॉडल जीव है. इस प्रोटोकॉल दीर्घायु के रूप में के रूप में अच्छी तरह से संभावित नुकसान की एक प्रतिलिपि प्रस्तुत करने योग्य, जनसंख्या के आधार पर माप और से बचने के लिए उन्हें कैसे पैदा करने में शामिल कदम पर चर्चा करेंगे.

उम्र के मापन में<em&gt; ड्रोसोफिला मेलानोगास्टर</em

Aging is a phenomenon that results in steady physiological deterioration in nearly all organisms in which it has been examined, leading to reduced ...

Research

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Biology

Measurement of Lifespan in Drosophila melanogaster

Dissection of Larval CNS in Drosophila Melanogaster

ड्रोसोफिला मेलानोगास्टर में लारवल सीएनएस के विच्छेदन

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

उच्च संकल्प प्रौढ़ ड्रोसोफिला मेलानोगास्टर में हरकत की वीडियो ट्रैकिंग

Flies provide an important model for studying complex behavior due to the plethora of genetic tools available to researchers in this field.  Studying ...

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

निगरानी लारवल में हार्ट फंक्शन ड्रोसोफिला मेलानोगास्टर शारीरिक अध्ययन के लिए

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

के लाइव इमेजिंग ड्रोसोफिला मेलानोगास्टर भ्रूण Hemocyte migrations

Many studies address cell migration using in vitro methods, whereas the physiologically relevant environment is that of the organism itself. Here we ...

Dissection of Oenocytes from Adult Drosophila melanogaster

Dissection of Oenocytes from Adult Drosophila melanogaster

वयस्क से Oenocytes के विच्छेदन ड्रोसोफिला मेलानोगास्टर</em

In Drosophila melanogaster, as in other insects, a waxy layer on the outer surface of the cuticle, composed primarily of hydrocarbon compounds, provides ...

Isolation of Drosophila melanogaster Testes

Isolation of Drosophila melanogaster Testes

के अलगाव ड्रोसोफिला मेलानोगास्टर Testes

The testes of Drosophila melanogaster provide an important model for the study of stem cell maintenance and differentiation, meiosis, and soma-germline ...

Imaging Through the Pupal Case of Drosophila melanogaster

Imaging Through the Pupal Case of Drosophila melanogaster

की पोटा संबंधी मामले के माध्यम से इमेजिंग<em&gt; ड्रोसोफिला मेलानोगास्टर</em

The longstanding use of Drosophila as a model for cell and developmental biology has yielded an array of tools. Together, these techniques have enabled ...

Measurement of Metabolic Rate in Drosophila using Respirometry

Measurement of Metabolic Rate in Drosophila using Respirometry

मेटाबोलिक दर के मापन में<em&gt; ड्रोसोफिला</em&gt; Respirometry का उपयोग

Metabolic disorders are a frequent problem affecting human health. Therefore, understanding the mechanisms that regulate metabolism is a crucial ...

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

छोटे आरएनए प्रजाति के उत्तरी ब्लाट पता लगाने में बढ़ी<em&gt; ड्रोसोफिला मेलानोगास्टर</em

The last decades have witnessed the explosion of scientific interest around gene expression control mechanisms at the RNA level. This branch of molecular ...

Maintenance of a Drosophila melanogaster Population Cage

Maintenance of a Drosophila melanogaster Population Cage

एक का रखरखाव<em&gt; ड्रोसोफिला मेलानोगास्टर</em&gt; जनसंख्या केज

Large quantities of DNA, RNA, proteins and other cellular components are often required for biochemistry and molecular biology experiments. The short life ...