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DOI :
10.3791/50076-v
June 23rd, 2013
Chapters
0:05
Title
1:28
T-cell Spreading Assay Preparation
2:52
T-cell Activation and Imaging
7:21
Imaging Data Analysis
8:42
Results: Live Imaging of SLP76 Deficient T-cells
9:48
Conclusion
我々は、T細胞活性化プロセスの間にタンパク質のダイナミクスについての洞察を提供して生細胞撮像方法を説明する。私たちは、定量的な結果は、T細胞活性化の全体の複合体形成のシグナルに従う得T細胞拡散アッセイ、共焦点顕微鏡と画像解析を組み合わせた使用法を示す。
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