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9.2K Views
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04:21 min
May 15th, 2013
DOI :
10.3791/50080-v
Chapters
0:05
Title
1:13
Reagent Preparation
2:29
Intratracheal Administration of Protein
3:36
Results: Protein Transfection in the Lung
3:58
Conclusion
Summary
转基因小鼠或病毒载体已用于肺内,以增加蛋白质的表达。然而,这些技术是费时,技术上具有挑战性,并有脱靶效应,可以混淆结果。蛋白转染协议使用基于脂质的转染的试剂和超细microsprayer的均匀地提供肺细胞的活性的蛋白质。
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