Name: multi-unit recording methods to characterize neural activity in the locust (schistocerca americana) olfactory circuits
Uploaded: 2013-01-25T12:00:00
Description: Watch this Scientific Journal Video about Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits at JoVE.com

作者想感谢以下为这项工作提供资金：大方的启动资金从生物医学工程系，美国华盛顿大学，麦中心系统神经科学经费，办公室美国海军研究资助（批准编号：N000141210089）到BR

1。气味准备工作和实施<ol><li>稀释在矿物油中的气味的解决方案（体积），以达到所需的浓度水平。存储一个20毫升的混合物，矿物油和的加臭剂，在60毫升的玻璃瓶。成的橡胶止动件（表19），一个从底部和其他从顶部插入两个注射器针头，以提供一个入口和一个出口管线。该橡胶塞密封的玻璃瓶中，并附加一个自定义设计的活性炭过滤器的入口线（ 图1A）。 </li><li>的碳过滤器是?...

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大多数感官刺激，唤起组合分布在歌舞团的神经元的反应。因此，同时监控多神经元活动是有必要了解如何刺激特定的信息在大脑中的神经回路表示和处理。在这里，我们已经证明细胞外多单位记录技术的气味诱发反应的特点前三加工中心以及昆虫嗅觉通路。我们注意到，这里所介绍的技术已嗅觉编码的以前的研究中使用的一些，并成为一个标准的实践在此字段中3,6,13-17。相结合的技术?...

<table border="1"><tbody><tr><td> 名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td></td><td></td><td></td><td> 电设备 </td></tr><tr><td> AC放大器</td><td> GRASS </td><td>型号P55 </td><td>单感器录音的</td></tr><tr><td>音频显示器（型号3300） </td><td> AM系统</td><td> 940000 </td><td></td></tr><tr><td>定做16个信道，前置放大器和放大器</td><td>卡尔。技术。生物电子商店</td><td></td><td> AL和MB录音</td></tr><tr><td>数据采集​​单元</td><td>美国国家仪器公司</td><td> BNC-2090 </td><td></td></tr><tr><td>光纤灯</td><td> WPI </td><td> SI-72-8 </td><td> &lt;/ TD&gt; </tr><tr><td>光源115 V </td><td> WPI </td><td> NOVA </td><td></td></tr><tr><td>手动显微操纵器</td><td> WPI </td><td> M3301R </td><td>蝗虫大脑录音的</td></tr><tr><td> Stereomicroscope1繁荣的立场</td><td>徕卡</td><td> M80 </td><td>蝗虫大脑录音的</td></tr><tr><td> Stereomicroscope2 </td><td>徕卡</td><td> M205C </td><td>单感器录音的</td></tr><tr><td>振动隔离表</td><td> TMC </td><td> 63-500系列</td><td></td></tr><tr><td>电动微操作机器人</td><td>萨特仪器</td><td> MP285 / T </td><td></td></tr><tr><td>示波器</td><td>泰克公司</td><td> TD2014B </td><td></td></tr><tr><td></td><td></td><td></td><td> 电极/建筑工具 &lt;/ TD&gt; 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multi-unit recording methods to characterize neural activity in the locust (schistocerca americana) olfactory circuits

嗅觉检测和解释的许多生物的生存是至关重要的。值得注意的是，跨门类的物种都有着惊人的相似嗅觉系统在进化的时间1，化学传感，生物方法进行了优化。在昆虫的嗅觉系统，增味剂，转嗅觉受体神经元（ORN）的天线，这将化学刺激的动作电位的列车。从ORNs的感官输入，然后转发至触角叶（AL;类似于脊椎动物的嗅球结构）。在AL，神经表征气味采取的形式分布在歌舞团的主要神经元（PNS，也被称为投射神经元）2,3的时空放电模式。随后处理的AL输出由肯扬细胞（角质形成）在下游蘑菇体（MB），相关联的结构与嗅觉记忆和学习4,5。她的E，我们提出了电生理记录技术，在这些嗅觉电路的监控气味诱发的神经反应。 首先，我们提出一个单一的感受器记录的方法来研究人口的ORNs 6,7气味诱发反应。我们讨论使用生理盐水填充尖锐的玻璃吸管作为电极细胞外监控ORN反应。接下来，我们提出了一种方法来使用商业16通道的电极细胞外PN反应。类似的方法使用一个定制的8路双绞线四极管展示了肯扬细胞记录8。我们提供详细的实验装置，目前这些技术代表记录的痕迹。

在图3D中示出一个单一的ORN两种不同醇的气味诱发反应。根据记录位置（放置的电极）的多单元录音感器类型，可以实现的。 图6A中所示的原料外从AL记录波形。可以观察到的动作电位或尖峰的幅度变化，来自不同的PN，在此电压跟踪。虽然蝗虫触角叶投射神经元有兴奋和抑制神经元，唯一的PN生成钠尖峰，可以检测到细胞外3。这一观察表明?...

Watch this Scientific Journal Video about Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits at JoVE.com

Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits

我们证明细胞外的多单元的记录技术中的前三个阶段的无脊椎动物的嗅觉路径表征气味诱发反应的变化。这些技术可以很容易地适应以及其他神经系统检查合奏活动。

多单位记录方法来描述蝗虫的神经活动（<em&gt; Schistocerca美洲</em&gt;）嗅觉电路

Detection and interpretation of olfactory cues are critical for the survival  of many organisms. Remarkably, species across phyla have strikingly similar ...

Research

JoVE Journal

Neuroscience

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

Surgical Implantation of Chronic Neural Electrodes for Recording Single Unit Activity and Electrocorticographic Signals

用于记录单机活动和Electrocorticographic信号的慢性神经电极植入手术

The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery.  Here we provide useful information for ...

科研

神经科学

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

阈上的神经活动的单细胞和单穗的分辨率的光学的方法记录

Signaling of information in the vertebrate central nervous system is often carried by populations of neurons rather than individual neurons. Also ...

Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings (GCMR) in the Insect Antennal Lobe

Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings GCMR in the Insect Antennal Lobe

用气相色谱 - 多单元的录音（GCMR）昆虫触角叶嗅觉挥发物的鉴定

All  organisms inhabit a world full of sensory stimuli that determine their  behavioral and physiological response to their environment. Olfaction is ...

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

运行轮活动在啮齿类动物的昼夜节律的记录和分析

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion

光遗传学的扰动激光照射半完好的神经活动<em&gt;果蝇</em&gt;幼虫运动

Drosophila larval locomotion is a splendid model system in developmental and physiological neuroscience, by virtue of the genetic accessibility of the ...

Using Single Sensillum Recording to Detect Olfactory Neuron Responses of Bed Bugs to Semiochemicals

使用单一感器录音来检测臭虫的嗅觉神经元的反应，以信息化学物质

The insect olfactory system plays an important role in detecting semiochemicals in the environment. In particular, the antennal sensilla which house ...

Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

神经元电活动外记录与神经活性物质在清醒小鼠微电泳应用相结合

Differences in the activity of neurotransmitters and neuromodulators, and consequently different neural responses, can be found between anesthetized and ...

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

通过的嗅球钙监控录像的变化温度引起的神经元电活动<em&gt;非洲爪蟾</em

The olfactory system, specialized in the detection, integration and processing of chemical molecules is likely the most thoroughly studied sensory system. ...

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

嗅球的四次免疫染色用于嗅觉感觉轴突分子识别码的可视化

The mouse olfactory system is often used to study mechanisms of neural circuit formation&#12288;because of its simple anatomical structure. An Olfactory ...

Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats

Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats

急性<em&gt;体内</em&gt;麻醉大鼠超导途径局部场电位和多单位活动的电生理记录

Converging evidence shows that many neuropsychiatric diseases should be understood as disorders of large-scale neuronal networks. To better understand the ...