Name: recording and analysis of circadian rhythms in running-wheel activity in rodents
Uploaded: 2013-01-24T13:00:00
Description: Watch this Scientific Journal Video about Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents at JoVE.com

作者要感谢全宗德拉RECHERCHE连接健康魁北克省（FRSQ），加拿大卫生研究院（CIHR），自然科学和工程研究理事会，加拿大（NSERC）的薪酬奖励，设备补助金，营运资金，和Concordia大学研究主席的计划（CRUC），以及在此稿件由简·斯图尔特博士颇有见地的反馈意见。

1。动物房<ol><li> 凯奇：为了记录的个人啮齿动物活动的运行轮，每个网箱应当拥有一个单一的啮齿动物和运行轮。由于正在运行的车轮可以被认为是一个形式丰富，在所有研究中的所有啮齿动物应该有类似的访问到正在运行的车轮。 </li><li> 床上用品的变化：动物的处理以及在笼子里或床上用品，都可以非光的昼夜节律的影响，21日至23日的 ，所以，笼网地板?...

<ol>
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监测日活动节律运行的车轮是最常用和最可靠的方法来评估在夜间啮齿类动物的主生物钟的输出。轮运行的活动，但是，只有一个，可以连续监测的行为和生理的许多方面。虽然绝大多数的磨合轮活动发生在夜间，超过30％的总觉醒发生在白天25,26。其他端点可用于评估，包括活动，食品斌方式，饮酒，睡眠，体温的昼夜节律。因此，根据性质的研究中，研究人员记录数的节奏同时进行。

<table border="1"&gt;<tbody&gt;<tr&gt;<td<strong&gt;的试剂名称</strong</td&gt;<td<strong&gt;公司</strong</td&gt;<td<strong&gt;产品目录号</strong</td&gt;<td<strong评论（可选）</strong</td&gt;</tr&gt;<tr&gt;<td&gt; Vitalview卡，配件及应用软件</td&gt;<td&gt;迷你发射器</td&gt;<td＃855-0030-00</td&gt;<td&gt;（弯管，OR，USA）</td&gt;</tr&gt;<tr&gt;<td&gt; DP24数据端口</td&gt;<td&gt;迷你发射器</td&gt;<td＃840-0024-00</td&gt;<td&gt;（弯管，OR，USA）</td&gt;</tr&gt;<tr&gt;<td&gt; QA4模块</td&gt;<td&gt;迷你发射器</td&gt;<td＃130-0050-00</td&gt;<td&gt;（弯管，OR，USA）</td&gt;</tr&gt;<tr&gt;<td&gt;磁性开关</td&gt;<td&gt;迷你发射器</td&gt;<td＃130-0015-00</td&gt;<td&gt;（弯管，OR，USA）</td&gt;</tr&gt;<tr&gt;<td&gt; C-50电缆组件</td&gt;<td&gt;迷你发射器</td&gt;<td＃060-0045-10</td&gt;<td&gt;（弯管，OR，USA）</td&gt;</tr&gt;<tr&gt;<td老鼠车轮总成</td&gt;<td&gt;迷你发射器</td&gt;<td＃640-0700-00</td&gt;<td&gt;（弯管，OR，USA）</td&gt;</tr&gt;<tr&gt;<td凯奇和托盘支持</td&gt;<td&gt;迷你发射器</td&gt;<td＃640-0400-00</td&gt;<td&gt;（弯管，OR，USA）</td&gt;</tr&gt;<tr&gt;<td&gt;可用切去笼</td&gt;<td&gt;迷你发射器</td&gt;<td＃664-2154-00</td&gt;<td&gt;（弯管，OR，USA）</td&gt;</tr&gt;<tr&gt;<td格地板笼</td&gt;<td&gt;迷你发射器</td&gt;<td＃676-2154-00</td&gt;<td&gt;（弯管，OR，USA）</td&gt;</tr&gt;<tr&gt;<td&gt;废盘</td&gt;<td&gt;迷你发射器</td&gt;<td＃684-2154-00</td&gt;<td&gt;（弯管，OR，USA）</td&gt;</tr&gt;<tr&gt;<td&gt;灯罩</td&gt;<td&gt;微晶科学</td&gt;<td＃R-101</td&gt;<td（多伦多，ON，加拿大）</td&gt;</tr&gt;<tr&gt;<td&gt; 4W荧光灯</td&gt;<td&gt;微晶科学</td&gt;<td＃F4T5/CW</td&gt;<td（多伦多，ON，加拿大）</td&gt;</tr&gt;<tr&gt;<td&gt;隔离室</td&gt;<td&gt;自定义建</td&gt;<td</td&gt;<td&gt; 28“H×20”W×28“D：½”黑三聚氰胺。</td&gt;</tr&gt;</tbody&gt;</table

recording and analysis of circadian rhythms in running-wheel activity in rodents

当啮齿动物可以免费使用在自己的笼子里一个跑轮，这轮自愿使用将取决于1-5天的时间。夜间啮齿动物，包括老鼠，仓鼠，老鼠，是活跃在夜间和白天的相对不活跃。许多其他的行为和生理的措施，也表现出的生活节奏，但在啮齿动物中，运行轮活动主生物钟，下丘脑的视交叉上核（SCN）的输出作为一个特别可靠和方便的措施。在一般情况下，通过一个过程被称为夹带，每天运行轮活动模式自然会与环境的明暗周期（LD周期， 例如 12小时光照：12小时暗）对齐。但昼夜节律是内源性产生的行为模式，表现出了〜24小时内，并坚持不断的黑暗。因此，在一个LD周期的情况下，记录和分析的行驶轮活动被用来确定主观时间 - 日期。因为这些导演的生物钟节律被称为主观时间天的昼夜时间（CT）。相反，当一个LD周期本，被称为天时间确定由环境LD周期给时的时间（ZT）。 虽然运行轮活动的昼夜节律，通常与SCN时钟6-8，也可能参与昼夜节律振荡器在许多其他地区的大脑和身体9-14日活动节律的调节。例如，生活节奏在食品预见性的活动不要求的SCN 15,16和取而代之的是，与额外的SCN振荡器17日至20日在活动中发生变化。因此，运行轮活动记录的行为提供了重要的信息不仅SCN的主时钟输出，同时也对额外的SCN振荡器的活动。下面我们DESCRIBE使用的设备和方法，记录，分析和显示啮齿类实验动物的自发活动节律昼夜。

<ol><li> 计算机程序：专门的计算机程序代的actograms和计算的昼夜周期中通常使用的。这些计划包括，但不限于，Actiview（弯，Minimitter，OR）和Circadia。 </li><li> actograms：Actograms提供每日的行驶轮活动模式的图解说明。有单密谋（x-轴= 24小时）和双绘制（x-轴= 48小时）actograms。这两种方法情节连续的天从上到下，但双绘制actograms情节两天就每一个水平线。具体而言，双，绘制actograms显示在最右...

Watch this Scientific Journal Video about Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents at JoVE.com

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

昼夜节律在自愿轮运行活动在哺乳动物中的紧密耦合的分子在大脑中的主时钟振荡。因此，这些生活节奏的行为可以被用来研究这个生物钟的功能，遗传，药物和环境因素的影响。

运行轮活动在啮齿类动物的昼夜节律的记录和分析

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

Research

JoVE Journal

Neuroscience

Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

含量自发活动，研究昼夜节律和睡眠参数果蝇</em

Most life forms exhibit daily rhythms in cellular, physiological and behavioral phenomena that are driven by endogenous circadian (&equiv;24 hr) ...

科研

神经科学

Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity

测量小鼠使用车轮运行活动的昼夜和急性对光反应

Circadian rhythms are physiological functions that cycle over a period of approximately 24 hours (circadian- circa: approximate and diem: day)1, 2.  They ...

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

移动硅探针的神经行为鼠害的大型记录

A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding of input-output relationships of ...

A Fully Automated and Highly Versatile System for Testing Multi-cognitive Functions and Recording Neuronal Activities in Rodents

一个多认知功能测试，并记录在啮齿动物的神经元活动的完全自动化和高度灵活的系统

We have developed a fully automated system for operant behavior testing and neuronal activity recording by which multiple cognitive brain functions can be ...

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

逆行荧光染料标记允许有针对性的外单细胞记录技术鉴定神经元<em&gt;在体内</em

The overall goal of this method is to record single-unit responses from an identified population of neurons. In vivo electrophysiological recordings from ...

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits

多单位记录方法来描述蝗虫的神经活动（<em&gt; Schistocerca美洲</em&gt;）嗅觉电路

Detection and interpretation of olfactory cues are critical for the survival  of many organisms. Remarkably, species across phyla have strikingly similar ...

Deep Brain Stimulation with Simultaneous fMRI in Rodents

深部脑刺激与同步功能磁共振成像在鼠害

In order to visualize the global and downstream neuronal responses to deep brain stimulation (DBS) at various targets, we have developed a protocol for ...

In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments

In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments

在鼠标空肠和结肠段肠系膜传入神经活性的体外记录

Afferent nerves not only convey information concerning normal physiology, but also signal disturbed homeostasis and pathophysiological processes of the ...

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

爪蟾Tectal 神经元全细胞膜片钳记录的制备与协议

The Xenopus tadpole retinotectal circuit, comprised of the retinal ganglion cells (RGCs) in the eye which form synapses directly onto neurons in the optic ...

The Power of Interstimulus Interval for the Assessment of Temporal Processing in Rodents

间刺激间隔对啮齿类动物时间处理评估的力量

Temporal processing deficits have been implicated as a potential elemental dimension of higher-level cognitive processes, commonly observed in ...