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10:32 min
March 4th, 2013
DOI :
10.3791/50215-v
Chapters
0:05
Title
2:21
Subcellualar Fractionation: Removal of Nuclei, Unlysed Cells, and Cellular Debris
4:08
Crude Mitochondria Isolation
6:16
Isolation of Mitochondria-associated ER Membrane, MAMs
9:14
Results: Western Blot Analysis of GEMs and MAMs
8:27
Extraction of Glycosphingolipid-enriched Microdomains, GEMs
10:01
Conclusion
Summary
此过程说明了如何分离从成年小鼠的大脑相关的线粒体的ER膜或MAMS和糖脂富集的微区部分从MAMS和线粒体的准备工作。
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