Name: angiogenesis in the ischemic rat lung
Uploaded: 2013-02-08T13:00:00
Description: Watch this Scientific Journal Video about Angiogenesis in the Ischemic Rat Lung at JoVE.com

我们认识到，工作堡，的博士Adlah的MD协助铸造的肺。这项工作已经由NHLBI，HL088005。

对老鼠进行的所有协议，由美国约翰·霍普金斯大学动物护理和使用委员会批准，并按照与美国国立卫生研究院的指导方针。只要有可能，动物应该是手术准备在从手术区域分开的区域的外科手术部位，以尽量减少污染。 1。麻醉/镇痛<ol><li>放置在感应腔注入3％异氟醚的大鼠（只SD雄性大鼠，125-150克;哈伦，印第安纳波利斯，IN）。 </li><li>将板的鼻锥和呼吸机连接到3％异氟?...

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左肺动脉结扎在所有物种进行测试导致的缺血性强大的全身新生血管的肺。我们已经提出了详细的手术方法在大鼠模型。我们的研究结果所产生血管铸型，组织病理学，并在体内标记表明支气管动脉增殖和灌注肺实质。因此，的支气管血管生成机制，可以研究平行的人类生存条件的慢性肺血栓栓塞症的动物模型。此外，临床上相关的小动物模型提供了一个机会，学习的形式和功能的新生血?...

全身血管生成的肺是大家公认的。在疾病状态下，如哮喘，肺间质纤维化3，癌症，慢性肺血栓栓塞症，全身血管和周围的肺中繁衍，侵入肺实质。然而，动物模型来研究这种差别激活的系统，而不是肺循环的毕竟是少数。也许全身新生血管在成年哺乳动物的肺是最可重复性的模型是发生诱导慢性肺动脉缺血后。左肺动脉阻塞在人类5-7，狗，猪，羊，豚鼠，大鼠，12日，13日和小鼠14支气管动脉和肋间动脉的快速普及。全身neovasculari负责的机制zation的肺肺缺血后，在很大程度上是未知的，并没有被广泛研究。支气管左肺动脉阻塞后的大鼠血管生成的过程中的时间已被仔细描述在病理工作威贝尔1。这项工作在大鼠延伸的，我们的实验室已集中生长的重要因素，以及在这个过程中的生理结果本新生血管在肺。结果表明缺血后早期和中和抗体治疗大鼠CXCR2的趋化因子CINC-3升高，CINC-3受体，抑制血管新生13。新成立的支气管肺缺血发作后的14天内被证明是血管异常显着提高蛋白质的通透性15。左肺功能弥散能力下降和减少肺容积15中的表现不正常。虽然新生血管可能有合作ntributed慢性肺缺血肺组织的保护，它似乎不正常，可能会导致肺功能持续下降。 这种模式最奇怪的方面之一可能涉及血管增殖的空间分布。尽管在肺实质因缺血而释放生长因子，新生血管起源于比较大的上游支气管动脉。出现正常的支气管动脉从主动脉的一个小分支和侵入呼吸道树，在气管隆突。这样的机制，其中生长因子诱导的初始阶段动脉生成是不明确的。我们建议，大鼠，用类似人类的血管解剖，提供一个独特的机会来研究负责全身血管生成在肺缺血的机制。虽然左肺动脉完全阻塞是一种罕见的发生在人类的主题，增加支气管血管患者出现同样引起任何部位和大小的肺动脉阻塞16。因此，我们提供了手术结扎左肺动脉在大鼠和的一种手段，以量化的大小的血管生成的方法的详细描述。

<table border="1"><tbody><tr><td> 试剂： </td><td> 公司 </td><td></td><td></td></tr><tr><td>盐酸丁丙诺啡，Puralube; </td><td>巴特勒施恩</td><td></td><td></td></tr><tr><td>布比卡因</td><td> APP制药公司</td><td></td><td></td></tr><tr><td>聚维酮碘棉棒</td><td> Dynarex公司</td><td></td><td></td></tr><tr><td>聚丙烯缝线大小为6-0，3/8圈反向切割针</td><td> MYCO医疗</td><td></td><td></td></tr><tr><td> PE20管材</td><td> Becton Dickinson公司</td><td></td><td></td></tr><tr><td> 15微米的深红色的聚苯乙烯fluorospheres </td><td> Invitrogen公司</td><td></td><td></td></tr><tr><td> 1毫升汉密尔顿玻璃注射器</td><td>汉密尔顿公司</td><td> &lt;/ TD&gt; <td></td></tr><tr><td> 设备： </td><td></td><td></td><td></td></tr><tr><td>精灵加注射泵</td><td>肯特科学</td><td></td><td></td></tr><tr><td>荧光分光光度计</td><td> Digilab </td><td></td><td></td></tr><tr><td>啮齿动物呼吸机型号683 </td><td>哈佛设备</td><td></td><td></td></tr><tr><td></td><td></td><td></td><td> 表1表的具体的试剂和设备。 </td></tr></tbody></table>

angiogenesis in the ischemic rat lung

成人肺灌注全身支气管动脉和整个下肢静脉回流流经肺动脉。在大多数肺癌病症，它是规模较小的全身血管，增强肺灌注需要作出响应，并显示强大的新生血管。已经显示出肺血管局部缺血诱导的肺动脉梗阻导致快速全身动脉血管生成的人以及在几个动物模型。虽然组织学评估过程中的时间支气管动脉增殖的影响进行了仔细的描述的1的威贝尔，机制，负责本组织生长的新船是不明确的。我们提供左肺动脉缺血诱导的大鼠，导致支气管新生血管的手术细节。血管生成的程度的定量的呈现的两个内的肺血管床的存在下，由于一个额外的挑战。方法确定新生血管形成的基础上标记的微球注射。

血管铸型：左肺动脉缺血大鼠的影响的结果是在图1中所示。显示的是甲基丙烯酸酯演员的支气管血管和广泛的血管，气道左边的树后的28天内LPAL。要获得此铸造，注射全身血管与甲基丙烯酸酯的混合物（红色），降主动脉逆行进入气管插管和注射一个白色的基于硅的材料。血管铸型提供了一个显着的肺支气管血管生成的可视化。特别值得一提的是大，曲折上游的支气管血管的左肺。?...

Watch this Scientific Journal Video about Angiogenesis in the Ischemic Rat Lung at JoVE.com

Angiogenesis in the Ischemic Rat Lung

肺灌注全身支气管动脉和肺动脉。在大多数肺病理学，它是较小的全身血管，显示了强大的新血管形成。停止肺血流量，促进轻快的支气管血管生成。我们提供诱导左肺动脉缺血，促进支气管新生血管的手术细节。

在缺血大鼠肺的血管生成

The  adult lung is perfused by both the systemic bronchial artery and the entire  venous return flowing through the pulmonary arteries. In most lung ...

Research

JoVE Journal

Medicine

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart

高频超声心动图：非侵入性的反复测量小鼠心脏缺血再灌注心肌劳损，收缩力，二尖瓣关闭不全的第一个证据

Ischemia-reperfusion (IR) was surgically performed in murine hearts which were then subjected to repeated imaging to monitor temporal changes in ...

科研

医学

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

利用磁共振成像，容积电脑断层扫描及超音波的裸鼠乳腺癌骨转移大鼠模型血管生成的多模态成像

Angiogenesis is an essential feature of cancer growth and metastasis formation. In bone metastasis, angiogenic factors are pivotal for tumor cell ...

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

内皮素-1诱导大脑中动脉闭塞模型缺血性中风激光多普勒血流仪在大鼠指导

Stroke is the number one cause of disability and third leading cause of death in the world, costing an estimated $70 billion in the United States in ...

Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate

视网膜脱离模型鼠害由透明质酸钠的视网膜下注射

Subretinal injection of sodium hyaluronate is a widely accepted method of inducing retinal detachment (RD). However, the height and duration of RD or the ...

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

纤维蛋白胶对小鼠肺移植研究肺特异性血管生成

Recent significant advances in stem cell research and bioengineering techniques have made great progress in utilizing biomaterials to regenerate and ...

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

的隔离方法<em&gt;离体</em&gt;肺灌注的大鼠模型：教训制定鼠EVLP课程中学

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed ...

Rat Model of Photochemically-Induced Posterior Ischemic Optic Neuropathy

光化学诱导后部缺血性视神经病变的大鼠模型

Posterior Ischemic optic neuropathy (PION) is a sight-devastating disease in clinical practice. However, its pathogenesis and natural history ...

Rat Heterotopic Abdominal Heart/Single-lung Transplantation in a Volume-loaded Configuration

大鼠异位心脏腹部/在卷装配置单肺移植

Herein, we describe a novel technique for heterotopic abdominal heart-lung transplantation (HAHLT) in rats. The configuration of the transplant graft ...