Name: september 2012: this month in jove
Uploaded: 2012-09-01T14:00:00
Duration: 5 min 54 s
Description: Watch this Scientific Journal Video about September 2012: This Month in JoVE at JoVE.com

<h2 class="jove_title"><a href="https://www.jove.com/video/3524">Doppler Optical Coherence Tomography of Retinal Circulation</a></h2>
Ou Tan1, Yimin Wang1, Ranjith K. Konduru2, Xinbo Zhang1, SriniVas R. Sadda2, David Huang1 1Department of Ophthalmology, Oregon Health and Science University , 2Department of Ophthalmology, University of Southern California
Total retinal blood flow is measured by Doppler optical coherence tomography and semi-automated grading software.
<h2 class="jove_title"><a href="https://www.jove.com/video/3886">Micropipette Aspiration of substrate-attached cells to estimate cell stiffness</a></h2>
Myung-Jin Oh1, Frank Kuhr1, Fitzroy Byfield2, Irena Levitan1 1Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois, 2Institute for Medicine and Engineering, University of Pennsylvania 
Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
<h2 class="jove_title"><a href="https://www.jove.com/video/4061">Time-lapse imaging of neuroblast migration in the mouse forebrain</a></h2>
Jivan Khlghatyan, Armen Saghatelyan The Cellular Neurobiology Unit, Centre de Recherche Universit&#233; Laval Robert-Giffard
We describe a protocol for real-time videoimaging of neuronal migration in the mouse forebrain. The migration of virally-labeled or grafted neuronal precursors was recorded in acute live slices using wide-field fluorescent imaging with a relatively rapid acquisition interval to study the different phases of cell migration, including the durations of the stationary and migration phases and the speed of migration.
<h2 class="jove_title"><a href="https://www.jove.com/video/4215">Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes</a></h2>
Vladimir A. Timoshevskiy, Atashi Sharma, Igor V. Sharakhov, Maria V. Sharakhova Department of Entomology, Virginia Tech
Among the three mosquito genera, namely Anopheles, Aedes, and Culex, physical genome mapping techniques were established only for Anopheles, whose members possess readable polytene chromosomes. For the genera of Aedes and Culex, however, cytogenetic mapping remains challenging because of the poor quality of polytene chromosomes. Here we present a universal protocol for obtaining high-quality preparations of mitotic chromosomes and an optimized FISH protocol for all three genera of mosquitoes.
<h2 class="jove_title"><a href="https://www.jove.com/video/4251">Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins</a></h2>
Jason M. Beckta1, 2, Scott C. Henderson3, Kristoffer Valerie1, 2, 4 1Department of Radiation Oncology, Virginia Commonwealth University, 2Department of Biochemistry &#38; Molecular Biology, Virginia Commonwealth University, 3Department of Anatomy &#38; Neurobiology, Virginia Commonwealth University, 4Massey Cancer Center, Virginia Commonwealth University
This protocol describes a method for visualizing a DNA double-strand break signaling protein activated in response to DNA damage as well as its localization during mitosis.
<h2 class="jove_title"><a href="https://www.jove.com/video/4350">Cell tracking using photoconvertible proteins during zebrafish development</a></h2>
Ver&#243;nica A. Lombardo, Anje Sporbert, Salim Abdelilah-Seyfried Max Delbr&#252;ck Center for Molecular Medicine
Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism.
<h2 class="jove_title"><a href="https://www.jove.com/video/4427">Driving Simulation in the Clinic: Testing visual exploratory behavior in daily life activities in patients with visual field defects </a></h2>
Johanna Hamel1, 2, Antje Kraft1, Sven Ohl3, Sophie De Beukelaer1, Heinrich J. Audebert1, 2, Stephan A. Brandt1 1Department of Neurology, Universit&#228;tsmedizin Charit&#233;, 2Center for Stroke Research Berlin (CSB), Universit&#228;tsmedizin Charit&#233;, 3Berlin School of Mind and Brain, Humboldt Universit&#228;t zu Berlin
Patients with visual deficits after stroke report about different constraints in daily life most likely due to variable compensatory strategies, which are difficult to differentiate in clinical routine. We present a clinical set-up which allows measurement of different compensatory head- and eye-movement-strategies and evaluating their effects on driving performance.

september 2012: this month in jove

今年9月在朱庇特，在柏林自由大学医学院的研究人员展示了一种新的方法研究中风患者如何补偿视野缺损。要做到这一点，我们利用驾驶模拟器带刹车，方向盘，转向信号。利用驾驶模拟软件和先进的眼动追踪，研究人员可以比较的注视行为的中风患者，为他们导航通过虚拟驾驶课程，与不同程度的复杂性。虽然大脑后动脉梗塞，可能会导致类似的视觉障碍患者，有些是能够浏览的驾驶课程开发代偿眼球运动，而其他人陷入危险的障碍，如野猪崩溃。通过分析代偿的患者注视行为，我们看到了巨大的潜力，为驾驶模拟为工具，以恢复中风病人试图克服盲目的点在他们的视野。 在与南加州大学合作，在美国俄勒冈州健康与科学大学眼科学系的研究人员提出了一种方法，测量总血流量在视网膜上使用多普勒光学相干断层扫描（OCT）。在视网膜中含有数以百万计，捕捉视觉的图像，并将其转换成电信号，通过视神经向大脑的神经元。血管进入视网膜在视神经盘，其中连接到视网膜视神经。这些血管供应氧气和营养物质，同时也消除浪费。在一些视网膜疾病（如糖尿病性视网膜病变）或青光眼（这会影响视神经），可能会出现异常的视网膜血管。因为这些疾病导致的不可逆转的视力下降，视网膜血流量的测量是非常有用的临床实践和研究。不同于传统的光学成像方法，如激光兴奋剂激光多普勒pler和超声彩色多普勒，OCT可以提供绝对测量视网膜的血流量，这些都是基于多普勒频移的光，这是从红血细胞，因为他们通过血管流后向散射。我们的作者展示了如何与多普勒OCT扫描视网膜和视神经盘的扫描，然后进行分级和分析DOCTORC软件，该软件的作者开发。这种方法年级的学生和方法之间的重现性良好。此外，在眼，青光眼，视网膜血流量的测量高度相关的视力丧失。因此，多普勒OCT是一个强大的工具，可用于眼科研究和临床实践。 同时，蚊虫季节，昆虫学系，在弗吉尼亚理工大学的研究人员在蚊子的基因组染色体定位展示出一种简单而强大的技术。超过40蚊属，包含数千种，研究人员特别感兴趣在按蚊 ， 白纹伊蚊 ， 致倦库蚊 ，因为它们含有的物种传播有害人类疾病。约90％已被映射到冈比亚按蚊基因组的染色体位置，但它是非常困难的准备合适的染色体利差伊蚊属和库蚊属细胞株和标准的技术。为了解决这个问题，我们的作者使用4龄蚊幼虫，成虫盘，生产出高品质的染色体利差。研究人员展示了如何剖析成虫盘，并准备合适的染色体准备的荧光原位杂交（FISH）。因此，可以伊蚊 ，库蚊， 按蚊属的蚊子基因组图谱。这种技术铺平了道路昆虫学家进行精确的染色体地图不仅蚊子，同时也为其他昆虫。 在研究中心魁北克省拉瓦尔大学的研究人员展示了一种方法在小鼠前脑的神经细胞迁移的跟踪。哺乳动物大脑中神经元的一个重要场所，是脑室下区，并通过新诞生的神经细胞迁移距​​离这方面的吻侧迁移流中的嗅球。一个立体定位注射逆转录病毒编码绿色荧光蛋白标记的细胞，然后使用急性切片的制备，间隔拍摄成像，图像分析相结合的，我们的作者可以计算出标记的神经母细胞的迁移速度。通过仔细跟踪轨迹沿血管细胞，这种方法可以帮助阐明不同的分子线索，影响细胞的迁移和细胞机制。 这个简短的摘要强调几个显着的视频，文章，将于今年9月在朱庇特。我们还提供跟踪斑马鱼photoconvertible荧光蛋白的细胞命运的方法，用微量检测细胞的刚度和成像的行为，蛋白质，DNA损伤响应。

Watch this Scientific Journal Video about September 2012: This Month in JoVE at JoVE.com

September 2012: This Month in JoVE

今年9月在朱庇特，在柏林自由大学医学院的研究人员展示了一种新的方法研究中风患者如何补偿视野缺损。要做到这一点，我们利用驾驶模拟器带刹车，方向盘，转向信号。利用驾驶模拟软件和先进的眼动追踪，研究人员可以比较的注视行为的中风患者，为他们导航通过虚拟驾驶课程，与不同程度的复杂性。虽然大脑后动脉梗塞，可能会导致类似的视觉障碍患者，有些是能够浏览的驾驶课程开发代偿眼球运动，而其他人陷入危险的障碍，如野猪崩溃。通过分析代偿的患者注视行为，我们看到了巨大的潜力，使用模拟驾驶作为一种工具来修复中风病人试图克服的盲点在他们的视觉科幻电致发光。

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This September in JoVE, researchers from the School of Medicine at the Free University of Berlin demonstrate a novel method for studying how stroke ...

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