Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
21.1K Views
•
12:49 min
March 15th, 2013
DOI :
10.3791/50245-v
Chapters
0:05
Title
1:24
Generating a Replicate and Freezing Flies
2:42
Collection and Lyophilization of Fly Heads
3:53
RNA-specific Procedures: Total RNA Extraction and mRNA Purification
7:23
Metabolite-specific Procedures: Methanol-chloroform Extraction
9:54
Representative Results
12:23
Conclusion
Summary
在这里,我们描述的程序,用于提取和纯化的mRNA和代谢物从果蝇头。我们应用这些技术,以便更好地了解潜在的神经元变性的细胞扰动。这些方法可以很容易地扩展并适用于其他的“组学”项目。
Explore More Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved