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10.6K Views
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08:36 min
April 12th, 2013
DOI :
10.3791/50248-v
Chapters
0:05
Title
1:34
Dissecting CD-1 E10.5 Embryos
3:18
Dispase II Treatment
4:13
Separating the Facial Ectoderm and Mesenchyme
6:37
Tissue Purity after Separation
7:26
Conclusion
Summary
A protocol for separation of embryo facial ectoderm and mesenchyme is described. We use Dispase II to treat whole embryos first, dissect whole facial prominences out, and then separate the facial ectoderm and mesenchyme.
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