Name: mouse short- and long-term locomotor activity analyzed by video tracking software
Uploaded: 2013-06-20T12:00:00
Description: Watch this Scientific Journal Video about Mouse Short- and Long-term Locomotor Activity Analyzed by Video Tracking Software at JoVE.com

Support: Denne forskning blev støttet af National Institutes of Health (DK064862, NS058525 og AA019357 til GGF).

1.. Kortsigtet LMA Set Up and Procedure <ol><li> Kortsigtet LMA bur oprettet <ol><li> Test kortsigtet LMA i hjemmet bur af en enkelt opstaldet mus. For at lette VTS-afhængig billedanalyse, bruge strøelse i høj kontrast med musen farve (dvs. mørkt farvet strøelse til hvide mus og lys farvet sengetøj for sorte mus). </li><li> Under kortvarig LMA test dækker bure med klare ¼ i plexiglas med 11 x ¼ i diameter huller for at støtte passende ventilation. </li></ol></li><li> Kortsig...

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	<li>Goulding, E. H., Schenk, A. K., Juneja, P., MacKay, A. W., Wade, J. M., Tecott, L. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+robust+automated+system+elucidates+mouse+home+cage+behavioral+structure.">A robust automated system elucidates mouse home cage behavioral structure.</a> Proc. Natl. Acad. Sci. U.S.A. 105, 20575-20582 (2008).</li><li>Kent, S., Bluth&#233;, R. M., Kelley, K. W., Dantzer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sickness+behavior+as+a+new+target+for+drug+development.">Sickness behavior as a new target for drug development.</a> Trends Pharmacol. Sci. 13, 24-28 (1992).</li><li>Dantzer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cytokine,+sickness+behavior,+and+depression.">Cytokine, sickness behavior, and depression.</a> Immunol. Allergy Clin. North Am. 29, 247-264 (2009).</li><li>Godbout, J. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exaggerated+neuroinflammation+and+sickness+behavior+in+aged+mice+following+activation+of+the+peripheral+innate+immune+system.">Exaggerated neuroinflammation and sickness behavior in aged mice following activation of the peripheral innate immune system.</a> FASEB J. 19, 1329-1331 (2005).</li><li>York, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+biobehavioral+and+neuroimmune+impact+of+low-dose+ionizing+radiation.">The biobehavioral and neuroimmune impact of low-dose ionizing radiation.</a> Brain Behav. Immun. 26, 218-227 (2012).</li><li>York, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Individually+ventilated+cages+cause+chronic+low-grade+hypoxia+impacting+mice+hematologically+and+behaviorally.">Individually ventilated cages cause chronic low-grade hypoxia impacting mice hematologically and behaviorally.</a> Brain Behav. Immun. 26, 951-958 (2012).</li><li>Jennings, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Refining+rodent+husbandry:+the+mouse.+Report+of+the+Rodent+Refinement+Working+Party.">Refining rodent husbandry: the mouse. Report of the Rodent Refinement Working Party.</a> Lab. Anim. 32, 233-259 (1998).</li><li>York, J. M., Blevins, N. A., Baynard, T., Freund, G. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+testing+methods+in+psychoneuroimmunology.">Mouse testing methods in psychoneuroimmunology.</a> Methods in Molecular Biology. 934, 243-276 (2012).</li><li>Dantzer, R., O'Connor, J. C., Freund, G. G., Johnson, R. W., Kelley, K. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=From+inflammation+to+sickness+and+depression:+when+the+immune+system+subjugates+the+brain.">From inflammation to sickness and depression: when the immune system subjugates the brain.</a> Nat. Rev. Neurosci. 9, 46-56 (2008).</li><li>Crawley, J. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+phenotyping+of+rodents.">Behavioral phenotyping of rodents.</a> Comp. Med. 53, 140-146 (2003).</li><li>Karelina, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Social+isolation+alters+neuroinflammatory+response+to+stroke.">Social isolation alters neuroinflammatory response to stroke.</a> Proc. Natl. Acad. Sci. U.S.A. 106, 5895-5900 (2009).</li></ol>

Symptomer på sygdom i mus omfatter sløvhed, tab af interesse for sociale og miljømæssige omgivelser, utilpashed og anoreksi 2-3. I mus og andre gnavere, kan LMA bruges til at evaluere sygdom efter immunaktivering 4-5. Derudover langsigtet LMA giver mulighed for at spore bevægelse over en 24 hr (eller højere) periode, så analysen af ​​behandlingen-inducerede ændringer i døgnrytmen adfærd eller forstyrrelser i søvn / vågner mønstre. LMA giver flere fordele frem for andre test for syg...

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		<tr height="21">
			<td height="21">Name of Reagent/Material</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="276">
			<td height="276">Video camera</td>
			<td>Sony</td>
			<td>HDR-XR500V</td>
			<td>Other, similar video cameras may work as well. The file storage format (codec/container) must be compatible with the VTS used. It is recommended when initially establishing a VTS to choose the VTS first and work with the vendor in choosing an appropriate camera, especially with respects to file format and video resolution compatibility.</td>
		</tr>
		<tr height="331">
			<td height="331">Opaque cage dividers</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Custom fabricated based on specific cage dimensions. To facilitate image analysis, dividers should be of a similar color to the cage bedding. Structurally fixed dividers are preferrable because they speed setup between multi-mice experiments, such as those involving repeated measurements. Sturdy transportable cage dividers can be constructed of 1/2 inch opaque plastic and joined via a series of cross laps.</td>
		</tr>
		<tr height="74">
			<td height="74">Light or Dark bedding (opposite of mouse coat color)</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Available from several laboratory animal supply distributors, depending on location.</td>
		</tr>
		<tr height="37">
			<td height="37">Clear Plexiglas cage tops</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Custom fabricated based on cage dimensions.</td>
		</tr>
		<tr height="92">
			<td height="92">Automated video tracking software (EthoVision XT 7)</td>
			<td>Noldus Information Technology</td>
			<td>&nbsp;</td>
			<td>A variety of VTS manufacturers exist, use of other software may suffice.</td>
		</tr>
		<tr height="55">
			<td height="55">PC</td>
			<td>Dell Inc.</td>
			<td>&nbsp;</td>
			<td>See VTS software providers minimum PC .requirements</td>
		</tr>
		<tr height="83">
			<td height="83">White noise generator</td>
			<td>Dohm</td>
			<td>DS</td>
			<td>A variety of white noise generators are available, depending on need.</td>
		</tr>
		<tr height="146">
			<td height="146">Brooder clamp lights with red light bulbs</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Several sizes and styles available. Number required depends on room size/lighting needs. Do not exceed 125 lumens in the testing room.</td>
		</tr>
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			<td height="62">Lab bench or butcher paper</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Paper should match or be very similar in color to the cage bedding.</td>
		</tr>
	</tbody>
</table>

mouse short- and long-term locomotor activity analyzed by video tracking software

Bevægelsesaktivitet (LMA) er en enkel og let udføres måling af adfærd hos mus og andre gnavere. Forbedringer i video tracking software (VTS) har gjort det muligt at blive koblet til LMA testning, dramatisk forbedring specificitet og sensitivitet i forhold til linjekrydsninger metode med manuel scoring. Derudover giver VTS high-throughput eksperimenteren. Mens ligner automatiseret Videosporingssystemet anvendes til åben mark test (OFT), LMA test er enestående, fordi det giver mulighed for mus til at forblive i deres hjem bur og ikke udnytter anxiogen stimulus af skarpt lys i den aktive fase af lys-mørke cyklus. Traditionelt har LMA blevet brugt til korte perioder (minutter), mens de længere bevægelse undersøgelser (timer-dage) ofte har brugt implanteret sendere og biotelemetry. Med mulighed for real-time tracking, lange, ligesom kortsigtet LMA test, kan nu udføres ved hjælp af videography. Langsigtet LMA test kræver en specialiseret, Men let bygget, bur, så mad og vand (som normalt er placeret på buret øverst) ikke hindrer videography. Vigtigere er det, videography og VTS giver mulighed for kvantificering af parametre, såsom vej musen, der er vanskelige eller umuligt at måle med line passage og / eller biotelemetry. I summen, giver LMA test koblet til VTS en mere fuldstændig beskrivelse af muse bevægelse og evnen til at undersøge bevægelse over en længere tidsperiode.

Et eksempel på resultaterne af gentagen foranstaltninger er kortvarig LMA test er vist i figur 1.. Dette tal viser, at efter (LPS) administration, mus huse i individuelt ventilerede bure (IVC) genvinde kortsigtede bevægelsesaktivitet hurtigere end mus opstaldet i omgivelserne bure (AEC) 6.. Mens den oprindelige tab af motorisk aktivitet er ens mellem LPS injicerede grupper, inddrivelse til basal LMA er hurtigere i IVC-opstaldet mus, som var hastigheden af bevægelse (figur 1b)</stro...

Watch this Scientific Journal Video about Mouse Short- and Long-term Locomotor Activity Analyzed by Video Tracking Software at JoVE.com

Mouse Short- and Long-term Locomotor Activity Analyzed by Video Tracking Software

Bevægelsesaktivitet (LMA) er en enkel og let udføres måling af adfærd hos mus. Kobling af video tracking software (VTS) og LMA muliggør forbedring af specificitet og sensitivitet, især sammenlignet med den manuelle, linie krydser metode LMA analyse. Derudover VTS tillader langsigtet sporing af muse LMA.

Mus kort og lang sigt motorisk aktivitet Analyzed by Video Tracking Software

Locomotor  activity (LMA) is a simple and easily performed measurement of behavior in mice  and other rodents. Improvements in video tracking software ...

Research

JoVE Journal

Behavior

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Automatiseret, langsigtede adfærdsmæssige Assay for kognitive funktioner i flere genetiske modeller af Alzheimers sygdom, ved hjælp af IntelliCage

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Domesticated animals such as dogs and laboratory mice show a high level of tameness, which is important for humans to handle them easily. Tameness has two ...

Elevated Plus Maze Test Combined with Video Tracking Software to Investigate the Anxiolytic Effect of Exogenous Ketogenic Supplements

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The overall goal of this study is to describe the methodology of the elevated plus maze (EPM) test in combination with a video tracking software. The ...

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Cave-dwelling animals have evolved a series of morphological and behavioral traits to adapt to their perpetually dark and food-sparse environments. Among ...

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High intensity interval training (HIIT) is emerging as a therapeutic approach to prevent, delay, or ameliorate frailty. In particular short session HIIT, ...

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Across phyla, sleep is characterized by highly conserved behavioral characteristics that include elevated arousal threshold, rebound following sleep ...