Name: mouse short- and long-term locomotor activity analyzed by video tracking software
Uploaded: 2013-06-20T12:00:00
Description: Watch this Scientific Journal Video about Mouse Short- and Long-term Locomotor Activity Analyzed by Video Tracking Software at JoVE.com

Support: Denne forskningen ble støttet av National Institutes of Health (DK064862, NS058525 og AA019357 til GGF).

En. Kortsiktig LMA Set Up og prosedyre <ol><li> Kortsiktig LMA bur satt opp <ol><li> Test kortsiktig LMA i hjemmet bur av en single-huset mus. For å lette VTS-avhengige bildeanalyse, bruker sengetøy i høy kontrast med musen farge (dvs. mørk farget sengetøy for hvite mus og lys farget sengetøy for svarte mus). </li><li> Under kortsiktig LMA testing, dekke bur med klare ¼ i pleksiglass med 11 x ¼ i diameter hull å støtte riktig ventilasjon. </li></ol></li><li> Kortsiktig LMA t...

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	<li>Goulding, E. H., Schenk, A. K., Juneja, P., MacKay, A. W., Wade, J. M., Tecott, L. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+robust+automated+system+elucidates+mouse+home+cage+behavioral+structure.">A robust automated system elucidates mouse home cage behavioral structure.</a> Proc. Natl. Acad. Sci. U.S.A. 105, 20575-20582 (2008).</li><li>Kent, S., Bluth&#233;, R. M., Kelley, K. W., Dantzer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sickness+behavior+as+a+new+target+for+drug+development.">Sickness behavior as a new target for drug development.</a> Trends Pharmacol. Sci. 13, 24-28 (1992).</li><li>Dantzer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cytokine,+sickness+behavior,+and+depression.">Cytokine, sickness behavior, and depression.</a> Immunol. Allergy Clin. North Am. 29, 247-264 (2009).</li><li>Godbout, J. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exaggerated+neuroinflammation+and+sickness+behavior+in+aged+mice+following+activation+of+the+peripheral+innate+immune+system.">Exaggerated neuroinflammation and sickness behavior in aged mice following activation of the peripheral innate immune system.</a> FASEB J. 19, 1329-1331 (2005).</li><li>York, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+biobehavioral+and+neuroimmune+impact+of+low-dose+ionizing+radiation.">The biobehavioral and neuroimmune impact of low-dose ionizing radiation.</a> Brain Behav. Immun. 26, 218-227 (2012).</li><li>York, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Individually+ventilated+cages+cause+chronic+low-grade+hypoxia+impacting+mice+hematologically+and+behaviorally.">Individually ventilated cages cause chronic low-grade hypoxia impacting mice hematologically and behaviorally.</a> Brain Behav. Immun. 26, 951-958 (2012).</li><li>Jennings, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Refining+rodent+husbandry:+the+mouse.+Report+of+the+Rodent+Refinement+Working+Party.">Refining rodent husbandry: the mouse. Report of the Rodent Refinement Working Party.</a> Lab. Anim. 32, 233-259 (1998).</li><li>York, J. M., Blevins, N. A., Baynard, T., Freund, G. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+testing+methods+in+psychoneuroimmunology.">Mouse testing methods in psychoneuroimmunology.</a> Methods in Molecular Biology. 934, 243-276 (2012).</li><li>Dantzer, R., O'Connor, J. C., Freund, G. G., Johnson, R. W., Kelley, K. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=From+inflammation+to+sickness+and+depression:+when+the+immune+system+subjugates+the+brain.">From inflammation to sickness and depression: when the immune system subjugates the brain.</a> Nat. Rev. Neurosci. 9, 46-56 (2008).</li><li>Crawley, J. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+phenotyping+of+rodents.">Behavioral phenotyping of rodents.</a> Comp. Med. 53, 140-146 (2003).</li><li>Karelina, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Social+isolation+alters+neuroinflammatory+response+to+stroke.">Social isolation alters neuroinflammatory response to stroke.</a> Proc. Natl. Acad. Sci. U.S.A. 106, 5895-5900 (2009).</li></ol>

Symptomer på sykdom hos mus er slapphet, tap av interesse for sosiale og miljømessige omgivelser, ubehag og anoreksi 2-3. Hos mus og andre gnagere kan LMA brukes til å vurdere sykdom etter aktivering av immunsystemet 4-5. I tillegg tilbyr langsiktige LMA muligheten til å spore bevegelse over en 24-timers (eller større) periode, slik at analyse av behandling-induserte endringer i circadian atferd eller forstyrrelser i søvn / våkne mønstre. LMA tilbyr flere fordeler fremfor andre tester for s...

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		<tr height="21">
			<td height="21">Name of Reagent/Material</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="276">
			<td height="276">Video camera</td>
			<td>Sony</td>
			<td>HDR-XR500V</td>
			<td>Other, similar video cameras may work as well. The file storage format (codec/container) must be compatible with the VTS used. It is recommended when initially establishing a VTS to choose the VTS first and work with the vendor in choosing an appropriate camera, especially with respects to file format and video resolution compatibility.</td>
		</tr>
		<tr height="331">
			<td height="331">Opaque cage dividers</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Custom fabricated based on specific cage dimensions. To facilitate image analysis, dividers should be of a similar color to the cage bedding. Structurally fixed dividers are preferrable because they speed setup between multi-mice experiments, such as those involving repeated measurements. Sturdy transportable cage dividers can be constructed of 1/2 inch opaque plastic and joined via a series of cross laps.</td>
		</tr>
		<tr height="74">
			<td height="74">Light or Dark bedding (opposite of mouse coat color)</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Available from several laboratory animal supply distributors, depending on location.</td>
		</tr>
		<tr height="37">
			<td height="37">Clear Plexiglas cage tops</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Custom fabricated based on cage dimensions.</td>
		</tr>
		<tr height="92">
			<td height="92">Automated video tracking software (EthoVision XT 7)</td>
			<td>Noldus Information Technology</td>
			<td>&nbsp;</td>
			<td>A variety of VTS manufacturers exist, use of other software may suffice.</td>
		</tr>
		<tr height="55">
			<td height="55">PC</td>
			<td>Dell Inc.</td>
			<td>&nbsp;</td>
			<td>See VTS software providers minimum PC .requirements</td>
		</tr>
		<tr height="83">
			<td height="83">White noise generator</td>
			<td>Dohm</td>
			<td>DS</td>
			<td>A variety of white noise generators are available, depending on need.</td>
		</tr>
		<tr height="146">
			<td height="146">Brooder clamp lights with red light bulbs</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Several sizes and styles available. Number required depends on room size/lighting needs. Do not exceed 125 lumens in the testing room.</td>
		</tr>
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			<td height="62">Lab bench or butcher paper</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Paper should match or be very similar in color to the cage bedding.</td>
		</tr>
	</tbody>
</table>

mouse short- and long-term locomotor activity analyzed by video tracking software

Lokomotorisk aktivitet (LMA) er en enkel og lett utføres måling av oppførsel i mus og andre gnagere. Forbedringer i video sporing (VTS) har tillatt det å bli koblet til LMA testing, dramatisk bedre spesifisitet og sensitivitet i forhold til linjen kryssinger metoden med manuell scoring. I tillegg gjør VTS high-throughput eksperimentering. Mens lik automatisert video sporing brukes for det åpne feltet test (OFT), er LMA testing unik ved at den tillater mus til å forbli i sine hjem bur og ikke utnytter den angstskapende stimulans av sterkt lys i løpet av den aktive fase av lys-mørk syklus. Tradisjonelt har LMA blitt brukt for korte perioder av gangen (min), mens lengre bevegelse studier (timer-dager) har ofte brukt implantert sendere og biotelemetry. Med mulighet for sanntids sporing, lang, som kortsiktig LMA testing, kan nå bli utført ved bruk av videoopptak. Langsiktig LMA testing krever en spesialisert, Men enkelt konstruert, bur, slik at mat og vann (som vanligvis er plassert på buret toppen) ikke hindrer videografi. Viktigste er at videography og VTS for kvantifisering av parametere, slik som banen musen bevegelse, som er vanskelig eller drivverdige å måle med linjen krysset og / eller biotelemetry. I sum, gir LMA testing koplet til VTS en mer fullstendig beskrivelse av mus bevegelse og evnen til å undersøke bevegelse over en forlenget tidsperiode.

Et eksempel på et resultat av en gjentatt tiltak, er kortvarig LMA test vist i figur 1.. Denne figuren viser at etter lipopolysakkarid (LPS) administrasjon, mus ligger i individuelt ventilert bur (IVC) gjenopprette kortsiktig bevegelsesaktiviteten raskere enn mus plassert i ambient miljø caging (AEC) 6. Mens den første tap av motorisk aktivitet er lik mellom LPS injisert grupper, er oppgangen til basal LMA raskere i IVC-plassert mus som var hastighet av bevegelse (Figur 1b)</strong...

Watch this Scientific Journal Video about Mouse Short- and Long-term Locomotor Activity Analyzed by Video Tracking Software at JoVE.com

Mouse Short- and Long-term Locomotor Activity Analyzed by Video Tracking Software

Lokomotorisk aktivitet (LMA) er en enkel og lett utføres måling av oppførsel i mus. Kobling av video sporing (VTS) og LMA åpner for forbedring av spesifisitet og sensitivitet, spesielt sammenlignet med den manuelle, linje krysset metode for LMA analyse. I tillegg VTS tillater langsiktig oppfølging av mus LMA.

Mus Kort-og langsiktige Locomotor aktivitet Analyzed by Video Tracking Software

Locomotor  activity (LMA) is a simple and easily performed measurement of behavior in mice  and other rodents. Improvements in video tracking software ...

Research

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