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12.1K Views
•
06:53 min
August 21st, 2013
DOI :
10.3791/50311-v
Chapters
0:05
Title
1:24
Generating K14-creER+ R26-SmoM2YFP+ Mice and Inducing SmoM2 Expression in Skin Keratinocytes
2:35
Skin Specimen Preparation and Epidermis Isolation
3:11
Single Cell Generation
3:54
Cell Labeling and Flow Cytometric Analysis
5:08
Results: Induction of SmoM2 Expression and Skin Keratinocytes
6:31
Conclusion
Summary
癌变是一个涉及癌细胞和肿瘤微环境之间的相互作用的方法。要解剖的分子事件,一个需要,隔离癌症的发展过程中的不同阶段的不同的细胞群。利用小鼠模型基底细胞癌癌变过程中,我们描述了一个协议,用于细胞分析。
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