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JoVE Journal

Neuroscience

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Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

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08:39 min

March 18th, 2013

March 18th, 2013

30,020 Views

0:05

Title

1:27

Preparation of Equipment and Virus Particle Suspension

3:09

Laminectomy

4:57

Injection

6:21

Wound Closure and Postoperative Care

7:35

Results: Transfection of Dorsal Horn Neurons in the Mouse Spinal Cord

8:08

Conclusion

Viral vectors allow for targeted gene manipulation. We demonstrate a method for conditional gene expression or ablation in the mouse spinal cord, using stereotaxic injection of a viral vector into the dorsal horn, a prominent site of synaptic contact between primary somatosensory afferents and neurons of the central nervous system.

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