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18.4K Views
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07:42 min
June 12th, 2013
DOI :
10.3791/50332-v
Chapters
0:05
Title
1:09
PBMC Isolation (Histopaque) and Cell Counting
2:55
Negative Isolation of Monocytes
5:09
Results: Representative Primary Human Macrophages
7:15
Conclusion
Summary
单核细胞源性巨噬细胞是先天免疫系统的重要细胞。这里,我们描述一个易于使用的在体外模型来生成这些细胞。使用梯度离心法,负胎圈分离和特定的细胞培养条件,可以产生单核细胞源性巨噬细胞表型和功能的研究。
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