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11:48 min
July 24th, 2013
DOI :
10.3791/50412-v
Chapters
0:05
Title
2:06
Preparation of DNA and Glass Micropipettes for Injection
2:57
Laparotomy
4:33
DNA Injection into the Brain Ventricle
5:59
Targeting the Hypothalamus for Electroporation
7:17
Finishing the Surgery
8:28
Analyzing the Results
9:18
Results: Hypothalamus Transfection by In utero Electroporation
10:54
Conclusion
Summary
視床下部の機能や医療の重要性にもかかわらず、子宮内遺伝子操作はほとんど試みられていない。私たちは、の詳細な手順を示す子宮内マウスの視床下部へのエレクトロポレーションと合計と部分(地域)視床下部のトランスフェクションの代表的な結果を示しています。
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