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19.3K Views
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10:22 min
July 13th, 2013
DOI :
10.3791/50436-v
Chapters
0:05
Title
1:27
Liposome Preparation and Detergent-induced Fusion of Vesicles
4:15
Removal of the Detergents to Form Proteoliposomes
6:28
Functional Study of the Ion Channel Activities in Black Lipid Membranes
8:49
Results: Two-Dimensional Crystallization of KvAP in Membranes
9:56
Conclusion
Summary
完整的原型脂膜的电压门控钾离子通道进入重组程序中描述的。重组的渠道都适合生化检测,电气录音,配体筛选和电子晶体学研究。这些方法可以有其它膜蛋白的结构和功能研究的一般应用。
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