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15.6K Views
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15:33 min
August 13th, 2013
DOI :
10.3791/50455-v
Chapters
0:05
Title
2:03
Cloning of a Gene of Interest into an Entry Vector
3:16
Generation of the Primary Virus Lysate
5:47
Virus Amplification
7:34
T Cell Infection
9:01
T Cell Activation and Polarization
10:01
T Cell Fixation Staining for Flow Cytometry
11:51
Infection Does Not Interfere with T Cell Activation
14:42
Conclusion
Summary
העברת גני adenoviral לתוך CD4 נאיבי תאי T מהונדס עם ביטוי של הקולטן אדנווירוס Coxsackie מאפשרת ניתוח המולקולרי של התמיינות תאי T רגולטורים במבחנה.
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