Cloning of a Gene of Interest into an Entry Vector
3:16
Generation of the Primary Virus Lysate
5:47
Virus Amplification
7:34
T Cell Infection
9:01
T Cell Activation and Polarization
10:01
T Cell Fixation Staining for Flow Cytometry
11:51
Infection Does Not Interfere with T Cell Activation
14:42
Conclusion
Adenoviral de transferência do gene em células T CD4 naive com a expressão transgénica do receptor de adenovirus Coxsackie possibilita a análise molecular da diferenciação de células T reguladoras In vitro.