Sign In

JoVE Journal

Neuroscience

A subscription to JoVE is required to view this content.

An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy

11.8K Views

11:52 min

June 13th, 2013

June 13th, 2013

11,783 Views

0:05

Title

2:23

Preparation of Mouse Vestibular Sensory Epithelium

6:11

Electrophysiology

7:44

Two-photon Microscopy

9:09

Results: Electrophysiological Recordings and Two-photon Calcium Imaging of the Semi-intact Mouse Vestibular Sensory Epithelium

11:21

Conclusion

Analysis of vestibular hair cell function is complicated by their location deep within the hardest part of the skull, the petrous temporal bone. Most functional hair cell studies have used acutely isolated hair cells. Here we describe a semi-intact preparation of mouse vestibular epithelium for electrophysiological and two-photon microscopy studies.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More