JoVE Logo
Faculty Resource Center

Sign In

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

DOI :

10.3791/50491-v

May 30th, 2013

May 30th, 2013

9,861 Views

1Department of Biological Sciences, Kingsborough Community College, 2Institute for Cellular and Molecular Biology, University of Texas at Austin, 3New Jersey Center for Science, Technology and Mathematics, Kean University

We set out to determine tolerable concentrations of three fatty acids (oleic, linoleic and linolenic acids) and cholera toxin that did not significantly and adversely affect cell survival by solubilizing the fatty acids and the toxin and using them in cell survival assays.

-- Views

Related Videos

article

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells

article

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

article

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model

article

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

article

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

article

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

article

Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

article

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

article

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms

article

Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved