July 4th, 2013
•Here we describe a protocol for optogenetic manipulation of motoneuronal activity while monitoring changes in motor output (muscle contraction) in semi-intact Drosophila larvae using lasers within a conventional confocal microscope. This technique enables researchers to achieve local perturbation of neural activity in a few neuromeres to elucidate the dynamics of motor circuits.
Related Videos
Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making
In vivo Laser Axotomy in C. elegans
Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae
Visualization of Proprioceptors in Drosophila Larvae and Pupae
Appetitive Associative Olfactory Learning in Drosophila Larvae
Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits
Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster
Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster
Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array
Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved