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Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

9.9K Views

11:31 min

September 18th, 2013

September 18th, 2013

9,897 Views

0:05

Title

2:02

Metabolic Labeling of Cells

5:50

Analysis of Protein Labeling

9:46

Results: Metabolic Labeling of LRRK1 and LRRK2

10:57

Conclusion

Leucine rich repeat kinases 1 and 2 (LRRK1 and LRRK2) are multidomain proteins which encode both GTPase and kinase domains and which are phosphorylated in cells. Here, we present a protocol to label LRRK1 and LRRK2 in cells with 32P orthophosphate, thereby providing a means to measure their overall cellular phophorylation levels.

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