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Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

30.7K Views

07:09 min

September 3rd, 2013

September 3rd, 2013

30,661 Views

0:05

Title

1:26

Preparation of Perfusion Equipment and Solutions

2:00

Perfusion of the Liver

4:29

Isolation of Hepatocytes

6:03

Isolated Hepatocyte Viability, Yield and Purity

6:47

Conclusion

A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.

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