Name: long-term potentiation of perforant pathway-dentate gyrus synapse in freely behaving mice
Uploaded: 2013-11-29T15:00:00
Description: Watch this Scientific Journal Video about Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice at JoVE.com

作者要感谢以下：约瑟夫Bronzino博士，博士哈米斯阿布 - 哈萨布拉，RJ奥斯汀LAFRANCE先生，和杰西卡Koranda女士。

这个协议是适当的为3至18个月的年龄和近似体重30-50克）的小鼠。老鼠可以从杰克逊实验室（缅因州巴港）获得。所有手术及实验方案已获本圣三一学院动物护理和使用委员会是根据美国国立卫生研究院指南实验动物的护理和使用。 1。动物的制备及外科手术<ol><li>制备含有氯胺酮（25毫克/毫升），甲苯噻嗪（2.5毫克/毫升）和乙酰丙嗪（0.5毫克/毫升）麻醉鸡尾酒。注射1毫升/公斤体重0.2毫?...

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	<li>Paxinos, G., Franklin, K. B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The mouse brain in stereotaxic coordinates. , (2004).</li><li>Bliss, T. V. P., Lomo, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-lasting+potentiation+of+synaptic+transmission+in+the+dentate+area+of+the+anesthetized+rabbit+following+stimulation+of+the+perforant+path.">Long-lasting potentiation of synaptic transmission in the dentate area of the anesthetized rabbit following stimulation of the perforant path.</a> J. Physiol. 232, 331-356 (1973).</li><li>Blaise, J. H., Bronzino, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+stimulus+frequency+and+age+on+bidirectional+synaptic+plasticity+in+the+dentate+gyrus+of+freely+moving+rats.">Effects of stimulus frequency and age on bidirectional synaptic plasticity in the dentate gyrus of freely moving rats.</a> Exp. Neurol. 182, 497-506 (2003).</li><li>Blaise, J. H., Koranda, J. L., Chow, U., Haines, K. E., Dorward, E. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neonatal+isolation+stress+alters+bidirectional+long-term+synaptic+plasticity+in+amygdalo-hippocampal+synapses+in+freely+behaving+adult+rats.">Neonatal isolation stress alters bidirectional long-term synaptic plasticity in amygdalo-hippocampal synapses in freely behaving adult rats.</a> Brain Res. 1193, 25-33 (2008).</li><li>Koranda, J. L., Masino, S. A., Blaise, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bidirectional+synaptic+plasticity+in+the+dentate+gyrus+of+the+awake+freely+behaving+mouse.">Bidirectional synaptic plasticity in the dentate gyrus of the awake freely behaving mouse.</a> J. Neurosci. Methods. 167, 160-166 (2008).</li><li>Cooke, S. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Autophosphorylation+of+alphaCaMKII+is+not+a+general+requirement+for+NMDA+receptor-dependent+LTP+in+the+adult+mouse.">Autophosphorylation of alphaCaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse.</a> J. Physiol. 574, 805-818 (2006).</li><li>Davis, S., Bliss, T. V., Dutrieux, G., Laroche, S., Errington, M. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+and+duration+of+long-term+potentiation+in+the+hippocampus+of+the+freely+moving+mouse.">Induction and duration of long-term potentiation in the hippocampus of the freely moving mouse.</a> J. Neurosci. Methods. 75, 75-80 (1997).</li><li>Jones, M. W., Peckham, H. M., Errington, M. L., Bliss, T. V., Routtenberg, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synaptic+plasticity+in+the+hippocampus+of+awake+C57BL/6+and+DBA/2+mice:+interstrain+differences+and+parallels+with+behavior.">Synaptic plasticity in the hippocampus of awake C57BL/6 and DBA/2 mice: interstrain differences and parallels with behavior.</a> Hippocampus. 11, 391-396 (2001).</li><li>Zhang, T. A., Tang, J., Pidoplichko, V. I., Dani, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Addictive+nicotine+alters+local+circuit+inhibition+during+the+induction+of+in+vivo+hippocampal+synaptic+potentiation.">Addictive nicotine alters local circuit inhibition during the induction of in vivo hippocampal synaptic potentiation.</a> J. Neurosci. 30, 6443-6453 (2010).</li><li>Tang, J., Dani, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dopamine+enables+in+vivo+synaptic+plasticity+associated+with+the+addictive+drug+nicotine.">Dopamine enables in vivo synaptic plasticity associated with the addictive drug nicotine.</a> Neuron. 63, 673-682 (2009).</li></ol>

在这个协议中，我们已经证明了在自由活动的小鼠研究LTP在DG可靠，简便的方法。而LTP的清醒大鼠许多研究已经进行3,4，很少有清醒小鼠主要是由于在小鼠颅有限，房地产和相对平均重量电极的探头的重量所带来的技术复杂性进行小鼠5。的几项研究已证明LTP的DG在自由活动利用任一微驱动电极系统或结型场效应晶体管集成在这必然增加了电极的有效载荷负担的动物6-10的探?...

技术的发展来操纵基因产生了几乎每一个神经变性疾病和神经系统疾病的转基因和基因敲除小鼠模型。因此有必要在以前在较大的啮齿动物到小鼠动物模型中使用的电生理研究技术翻译。一个这样的神经电生理研究技术是利用长时程增强（LTP）来测试参与各种神经病理疾病的神经元网络中的突触连接的有效性。本协议描述技术LTP的自由活动的小鼠可靠的电生理研究。此协议的比其他的优点在于，它是简单和容易实现的，它也是相当少昂贵，因为它不需要也不使用昂贵的计算机控制的微驱动系统也不场效应晶体管的探头的，并且，据我们所知，是慢性电生理记录吨第一视频协议Ø研究LTP在自由活动的小鼠。为此，我们在本文中描述的简单方法用于研究长时程增强中自由活动的小鼠。这些方法可以很容易地转换成神经病理性疾病的转基因和基因敲除小鼠模型。

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long-term potentiation of perforant pathway-dentate gyrus synapse in freely behaving mice

的突触效能的长时程增强的研究，其特性使其作为一个潜在的细胞机制基本学习和信息存储吸引力的活动依赖的突触现象，早已被用来阐明各种神经回路中的海马，杏仁核的生理和其他边缘和皮质结构。考虑到这一点，神经系统疾病的转基因小鼠模型代表有用的平台上进行长时程增强（LTP）的研究开发基因在正常和异常的突触通信中参与学习，情感和信息的神经元网络中的作用更深入的了解处理。本文介绍的方法进行可靠地诱导LTP在自由活动的小鼠。这些方法可以在转基因的研究中使用和敲除自由活动的神经退行性疾病的小鼠模型。

表1示出了如在此协议中使用的坐标为DG和MPP 图1A示出的标记为在头骨的目标结构;。还示出的是接地电极和参考电极的位置， 图1B示出了代表性的诱发反应的痕迹都预和posttetanization在相同的动物。注意，posttetanization诱发反应比pretetanization响应其表示LTP诱导2。 图1C示出了用于量化的振幅响应的方法大。实际上， 图2示出了响应...

Watch this Scientific Journal Video about Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice at JoVE.com

Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice (Video) | JoVE

神经系统疾病的转基因和基因敲除小鼠模型可用于研究基因在正常和异常的神经生理学中的作用是有用的。本文介绍的方法可用于研究长时程增强，它可依据学习和记忆，在转基因和基因敲除自由活动的神经病理学的小鼠模型细胞机制。

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Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice

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