Name: long-term potentiation of perforant pathway-dentate gyrus synapse in freely behaving mice
Uploaded: 2013-11-29T15:00:00
Description: Watch this Scientific Journal Video about Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice at JoVE.com

Forfatterne ønsker at anerkende følgende: Dr. Joseph Bronzino, Dr. Khamis Abu-Hassaballah Mr. RJ Austin-LaFrance, og Ms Jessica Koranda.

Denne protokol er egnet til mus af 3 og 18 måneders alderen, og omtrentlig legemsvægt på 30-50 g). Mus kan fås fra The Jackson Laboratory (Bar Harbor, ME). Alle kirurgiske og forsøgsprotokoller blev godkendt af Trinity College Animal Care og brug Udvalg og var i overensstemmelse med NIH Guide til Pleje og anvendelse af forsøgsdyr. 1.. Animal Forberedelse og kirurgiske procedurer <ol><li> Forbered anæstesi cocktail indeholdende ketamin (25 mg / ml), xylazin (2,5 mg / ml) og acepromazin (0,5 mg / ml). Inj...

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	<li>Paxinos, G., Franklin, K. B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The mouse brain in stereotaxic coordinates. , (2004).</li><li>Bliss, T. V. P., Lomo, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-lasting+potentiation+of+synaptic+transmission+in+the+dentate+area+of+the+anesthetized+rabbit+following+stimulation+of+the+perforant+path.">Long-lasting potentiation of synaptic transmission in the dentate area of the anesthetized rabbit following stimulation of the perforant path.</a> J. Physiol. 232, 331-356 (1973).</li><li>Blaise, J. H., Bronzino, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+stimulus+frequency+and+age+on+bidirectional+synaptic+plasticity+in+the+dentate+gyrus+of+freely+moving+rats.">Effects of stimulus frequency and age on bidirectional synaptic plasticity in the dentate gyrus of freely moving rats.</a> Exp. Neurol. 182, 497-506 (2003).</li><li>Blaise, J. H., Koranda, J. L., Chow, U., Haines, K. E., Dorward, E. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neonatal+isolation+stress+alters+bidirectional+long-term+synaptic+plasticity+in+amygdalo-hippocampal+synapses+in+freely+behaving+adult+rats.">Neonatal isolation stress alters bidirectional long-term synaptic plasticity in amygdalo-hippocampal synapses in freely behaving adult rats.</a> Brain Res. 1193, 25-33 (2008).</li><li>Koranda, J. L., Masino, S. A., Blaise, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bidirectional+synaptic+plasticity+in+the+dentate+gyrus+of+the+awake+freely+behaving+mouse.">Bidirectional synaptic plasticity in the dentate gyrus of the awake freely behaving mouse.</a> J. Neurosci. Methods. 167, 160-166 (2008).</li><li>Cooke, S. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Autophosphorylation+of+alphaCaMKII+is+not+a+general+requirement+for+NMDA+receptor-dependent+LTP+in+the+adult+mouse.">Autophosphorylation of alphaCaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse.</a> J. Physiol. 574, 805-818 (2006).</li><li>Davis, S., Bliss, T. V., Dutrieux, G., Laroche, S., Errington, M. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+and+duration+of+long-term+potentiation+in+the+hippocampus+of+the+freely+moving+mouse.">Induction and duration of long-term potentiation in the hippocampus of the freely moving mouse.</a> J. Neurosci. Methods. 75, 75-80 (1997).</li><li>Jones, M. W., Peckham, H. M., Errington, M. L., Bliss, T. V., Routtenberg, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synaptic+plasticity+in+the+hippocampus+of+awake+C57BL/6+and+DBA/2+mice:+interstrain+differences+and+parallels+with+behavior.">Synaptic plasticity in the hippocampus of awake C57BL/6 and DBA/2 mice: interstrain differences and parallels with behavior.</a> Hippocampus. 11, 391-396 (2001).</li><li>Zhang, T. A., Tang, J., Pidoplichko, V. I., Dani, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Addictive+nicotine+alters+local+circuit+inhibition+during+the+induction+of+in+vivo+hippocampal+synaptic+potentiation.">Addictive nicotine alters local circuit inhibition during the induction of in vivo hippocampal synaptic potentiation.</a> J. Neurosci. 30, 6443-6453 (2010).</li><li>Tang, J., Dani, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dopamine+enables+in+vivo+synaptic+plasticity+associated+with+the+addictive+drug+nicotine.">Dopamine enables in vivo synaptic plasticity associated with the addictive drug nicotine.</a> Neuron. 63, 673-682 (2009).</li></ol>

I denne protokol, har vi vist en pålidelig og enkel metode til at studere LTP i GD i frit opfører mus. Mens mange undersøgelser af LTP i vågne rotter er blevet udført 3,4, har meget få er udført i vågne mus primært på grund af den tekniske kompleksitet forbundet med den begrænsede kranie fast ejendom i mus, og vægten af elektrode headstages i forhold til den gennemsnitlige vægt af mus 5.. De få undersøgelser, der har påvist LTP i GD i frit opfører mus udnyttes enten Microdrive elek...

Udviklingen af ​​teknologien til at manipulere gener har produceret transgene og knockout musemodeller for næsten alle neurodegenerative og neurologiske sygdomme. Dette har nødvendiggjort oversættelse af elektrofysiologiske forskning teknikker tidligere blev anvendt i større gnavere til mus dyremodel. En sådan neurofysiologiske undersøgelse teknik er brugen langsigtet potensering (LTP) for at teste effektiviteten af ​​synaptiske forbindelser inden neuronale netværk, der er involveret i forskellige neuropatologiske lidelser. Denne protokol beskriver teknikker til pålidelig elektrofysiologisk undersøgelse af LTP i frit opfører mus. Fordelen ved denne protokol over andre, er, at den er enkel og let at implementere, og det er også temmelig billigere, da det ikke kræver hverken anvendelsen af ​​dyre computerstyrede Microdrive systemer eller felteffekttransistor headstages, samt i vores viden, er den første video-protokollen af ​​kroniske elektrofysiologiske optagelser to studere LTP i frit opfører mus. Til dette formål, vi beskriver i denne artikel enkle metoder til at studere langsigtede potensering i frit opfører mus. Disse metoder kan let oversættes til transgene og knockout musemodeller af neuropatologiske lidelser.

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			<td height="42" style="height:42px;width:195px;">Current isolation unit</td>
			<td style="width:95px;">Astro-Med, Inc.</td>
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long-term potentiation of perforant pathway-dentate gyrus synapse in freely behaving mice

Undersøgelser af langvarig potensering af synaptisk effektivitet, en aktivitet, der er afhængige synaptiske fænomen har egenskaber, der gør det attraktivt som en potentiel cellulære mekanisme underliggende læring og lagring af oplysninger, har længe været brugt til at belyse fysiologi af forskellige neuronale kredsløb i hippocampus, amygdala og andre limbiske og corticale strukturer. Med dette i tankerne, transgene musemodeller for neurologiske sygdomme udgør nyttige platforme for at gennemføre langsigtet (LTP) undersøgelser til at udvikle en større forståelse af den rolle, gener i normal og unormal synaptisk kommunikation i neuronale netværk, der er involveret i læring, følelser og information forarbejdning. Denne artikel beskriver metoder til pålidelig inducere LTP i frit opfører musen. Disse metoder kan anvendes i studier af transgene og knockout frit opfører musemodeller af neurodegenerative sygdomme.

Tabel 1 viser koordinaterne til GD og MPP som anvendes i denne protokol Figur 1A viser markeringerne for målet strukturer på kraniet.. Også vist er placering af jorden og reference elektroder 1B illustrerer repræsentant fremkaldt reaktion spor både præ- og posttetanization i det samme dyr. Bemærk, at posttetanization fremkaldt reaktion er større end pretetanization reaktion, som er vejledende for LTP induktion 2. Figur 1C illustrerer ...

Watch this Scientific Journal Video about Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice at JoVE.com

Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice

Transgene og knockout-musemodeller af neurologiske sygdomme er anvendelige til at studere rollen af ​​gener i normale og unormale neurofysiologi. Denne artikel beskriver metoder, der kan anvendes til at studere langsigtet potensering, en cellulær mekanisme, som kan være afgørende for indlæring og hukommelse i transgene og knockout frit opfører musemodeller for neuropatologi.

Langsigtet Potentiering af perforante Pathway-dentatgyrus Synapse i frit Opfører Mus

Studies of long-term potentiation of synaptic efficacy, an activity-dependent synaptic phenomenon having properties that make it attractive as a potential ...

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