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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

33.6K Views

12:36 min

February 16th, 2014

February 16th, 2014

33,643 Views

0:05

Title

1:14

Growing Bacterial Host (BLT5403) for Titering

2:15

Affinity Selection

8:13

Titering

9:41

Plaque Isolation

10:59

Results: Typical Titer and PCR Results for Affinity Selection over an Appropriate Bait

12:12

Conclusion

Phage display is a powerful technique to capture proteins or protein moieties that interact with an immobilized molecule of interest. Once a decision of the type of phage cDNA library to create and screen has been made, the protocol described here permits efficient affinity selection leading to identification of interactors.

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